SNX27抗体 (F-2) 推荐用于 mouse, rat 和 human 来源的SNX27 WB, IP, IF 和 ELISA检测

SNX27 抗体 (F-2): sc-515707

SNX27抗体 (F-2) is rated 3.0 out of 5 by 2.
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说明书

    • 抗-SNX27 抗体 F-2 是小鼠单克隆 IgG2a (kappa轻链),SNX27抗体,规格为200 µg/ml
    • 特异性抗原位于human物种的SNX27的C-terminus附近的氨基酸449-466之间
    • 抗-SNX27 抗体 (F-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 SNX27
    • 抗-SNX27 抗体 (F-2) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取SNX27 (F-2): sc-515707免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

What is the recommended starting dilution for Western blot for SNX27 (F-2): sc-515707 monoclonal antibody?

Asked by: TinTin
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-515707.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from Didn't work for me I used this AB for WesternBlots with MCF7 whole cell lysate Poor signal detected by ECL, several bands, nothing specific. Not better with high sensitivity detection reagent : more bands but nothing specific.
Date published: 2020-07-31
Rated 5 out of 5 by from Nice Strong bands in WB with NIH/3T3 and 3T3-L1 whole cell lysates,clean,nice.
Date published: 2017-01-05
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