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SH2-B Antibody (E-8) - Western Blotting - Image 295733
SH2-B 抗体 (E-8): sc-393395. SJRH30 (A), ES-2 (B) 和 OV-90 (C) 全细胞裂解液中 SH2-B 表达的 Western 印迹分析.
SH2-B Antibody (E-8) - Western Blotting - Image 295733
SH2-B 抗体 (E-8): sc-393395. SJRH30 (A), ES-2 (B) 和 OV-90 (C) 全细胞裂解液中 SH2-B 表达的 Western 印迹分析.
SH2-B Antibody (E-8) - Western Blotting - Image 366909
SH2-B 抗体 (E-8): sc-393395. Western 印迹分析 A-673 全细胞裂解液中 SH2-B 的表达.
SH2-B 抗体 (E-8): sc-393395. SJRH30 (A), ES-2 (B) 和 OV-90 (C) 全细胞裂解液中 SH2-B 表达的 Western 印迹分析.

SH2-B 抗体 (E-8): sc-393395

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寫評論提問

说明书
  • SH2-B 抗体 E-8 是小鼠单克隆 IgG1 κ，SH2-B抗体, 在1篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列334-422位于human物种的SH2-B的内部区域内
  • SH2-B 抗体 (E-8) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 SH2-B
  • 抗SH2-B抗体（E-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SH2-B (E-8): sc-393395的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是SH2-B Antibody (E-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与SH2-B Antibody (E-8) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

SH2-B抗体（E-8）是一种IgG1 κ小鼠单克隆SH2-B抗体（也称为SH2-B抗体），可通过WB、IP、IF和ELISA方法检测到来自人类的SH2-B蛋白。SH2-B抗体（E-8）既有非偶联形式的抗SH2-B抗体，也有多种偶联形式的抗SH2-B抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。SH2-B，也被称为SH2B1或PSM，是一种由756个氨基酸组成的蛋白质，是信号网络的一个组成部分，参与细胞形态和运动的调节。SH2-B与APS（含有PH和SH2结构域的适配分子）家族的适配蛋白相关，这类蛋白的特征是含有一个血小板同源（PH）结构域、一个SH2结构域和一个酪氨酸磷酸化位点。SH2-B通过选择性剪接产生三种不同的异构体，即SH2-B α、β和γ，它们具有相同的N端序列，包括PH结构域、SH2结构域和多个富含脯氨酸的模体。由于含有一个PH结构域和一个SH2结构域，SH2-B在细胞核和细胞质之间穿梭。SH2-B广泛表达，在骨骼肌和卵巢中的表达水平最高。SH2-B在酪氨酸残基上磷酸化以响应受体激酶的刺激。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SH2-B 抗体 (E-8) 参考文献:

  1. 神经生长因子诱导的神经元分化需要 SH2-B。  |  Rui, L., et al. 1999. J Biol Chem. 274: 10590-4. PMID: 10187854
  2. 鉴定 SH2-bbeta 是酪氨酸激酶 Janus 激酶 2 的强效细胞质激活剂。  |  Rui, L. and Carter-Su, C. 1999. Proc Natl Acad Sci U S A. 96: 7172-7. PMID: 10377387
  3. APS是一种具有PH和SH2结构域的适配蛋白，是胰岛素受体激酶的底物。  |  Ahmed, Z., et al. 1999. Biochem J. 341 (Pt 3): 665-8. PMID: 10417330
  4. SH2-B是一种膜相关适配器，在神经生长因子的作用下，会被MEK/ERK级联内的激酶磷酸化多个丝氨酸/苏氨酸。  |  Rui, L., et al. 1999. J Biol Chem. 274: 26485-92. PMID: 10473609
  5. SH2-beta的SH2结构域和N-末端区域与JAK2的不同结合和调控。  |  Rui, L., et al. 2000. Mol Cell Biol. 20: 3168-77. PMID: 10757801
  6. 与生长激素受体偶联所需的 JAK2 酪氨酸激酶区域。  |  Frank, SJ., et al. 1995. J Biol Chem. 270: 14776-85. PMID: 7540178
  7. 确定 SH2-Bbeta 是参与生长激素信号传导的酪氨酸激酶 JAK2 的底物。  |  Rui, L., et al. 1997. Mol Cell Biol. 17: 6633-44. PMID: 9343427
  8. 血小板衍生生长因子（PDGF）会刺激 SH2-Bbeta 与 PDGF 受体结合并使 SH2-Bbeta 磷酸化。  |  Rui, L. and Carter-Su, C. 1998. J Biol Chem. 273: 21239-45. PMID: 9694882

订购信息

产品名称产品编号规格价格数量收藏夹

SH2-B 抗体 (E-8)

sc-393395
200 µg/ml
$316.00

SH2-B (E-8): m-IgGκ BP-HRP 套装

sc-523928
200 µg Ab, 40 µg BP
$354.00

SH2-B (E-8): m-IgG1 BP-HRP 套装

sc-533626
200 µg Ab; 20 µg BP
$354.00

SH2-B 抗体 (E-8) AC

sc-393395 AC
500 µg/ml, 25% agarose
$416.00

SH2-B 抗体 (E-8) HRP

sc-393395 HRP
200 µg/ml
$316.00

SH2-B 抗体 (E-8) FITC

sc-393395 FITC
200 µg/ml
$330.00

SH2-B 抗体 (E-8) PE

sc-393395 PE
200 µg/ml
$343.00

SH2-B 抗体 (E-8) Alexa Fluor® 488

sc-393395 AF488
200 µg/ml
$357.00

SH2-B 抗体 (E-8) Alexa Fluor® 546

sc-393395 AF546
200 µg/ml
$357.00

SH2-B 抗体 (E-8) Alexa Fluor® 594

sc-393395 AF594
200 µg/ml
$357.00

SH2-B 抗体 (E-8) Alexa Fluor® 647

sc-393395 AF647
200 µg/ml
$357.00

SH2-B 抗体 (E-8) Alexa Fluor® 680

sc-393395 AF680
200 µg/ml
$357.00

SH2-B 抗体 (E-8) Alexa Fluor® 790

sc-393395 AF790
200 µg/ml
$357.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with SH2-B (E-8): sc-393395?

Asked by: DefinitelyNotMatt
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Immunoblot of the endogenous SH2B1 in PC12 cellsAnti-SH2B1 (E8) against aa 334-432 of human SH2B1 (sc.393395) PC12 cells stably expressing GFP tag (only endogenous SH2B1 present). Treated ± NGF (100 ng/ml), cells lysed in RIPA buffer (1% Triton X100). Load 40 ul on minigel. Immunoblot Block with 3% BSA in TBS-1% tween Blot anti-SH2B1 (E8) (1/250 dilution) 2o antibody mIGGkBP-HRP (sc) (1/1000) Nice SH2B1 signal detected with NGF-dependent shift in migration. Band at 90 kDa in absence of NGF. Band at 100 kDa in sample treated with NGF. When PC12 cells stably expressing GFP-SH2B1 beta were used, a dark band was detected at ~125 kDa which was not detected in PC12 expressing GFP alone. When human SH2B1, rat SH2B1, or mouse SH2B1 were expressed in 293T cells, the human SH2B1 and the rat SH2B1 were detected by the (E8) SH2B1 antibody. Mouse SH2B1 was not recognized. A study using 293T cells expressing SH2B1 with various truncations demonstrated that the (E8) SH2B1 antibody recognizes aa 269-397 of SH2B1. Since anti-SH2B1 (E8) was prepared against aa 334-432, from a Clustal W plot for human, rat and mouse the region between aa 334 and 397, the human sequence differs from the mouse sequence at aa 356, 359, 362, and 383. It is likely the epitope for anti-SH2B1 (E8) includes some of these residues.
Date published: 2018-08-23
Rated 5 out of 5 by from Very good Western blot data of SH2Very good Western blot data of SH2-B expression in SJRH30, ES-2 and OV-90 whole cell lysates. -SCBT QC
Date published: 2013-10-10
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SH2-B 抗体 (E-8) is rated 5.0 out of 5 by 2.
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