Date published: 2025-9-7
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SC Antibody (C-2) - Immunofluorescence - Image 149402
SC 抗体 (C-2): sc-374343. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示细胞质定位.
SC 抗体 (C-2): sc-374343. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示细胞质定位.

SC 抗体 (C-2): sc-374343

4.2(5)
寫評論提問

说明书
  • SC 抗体 C-2 是小鼠单克隆 IgG1 κ，SC抗体, 在6篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列465-764位于human物种的SC的C-terminus
  • SC 抗体 (C-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SC
  • 抗SC抗体（C-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SC (C-2): sc-374343的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 SC 抗体 (C-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SC 抗体 (C-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

SC抗体（C-2）是一种IgG1 κ鼠单克隆SC抗体（也称为SC抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人的SC蛋白。SC抗体（C-2）既可以是非偶联的抗SC抗体形式，也可以是多种偶联形式的抗SC抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。聚合IgA和IgM由位于极化上皮细胞黏膜下层的固有层中的B细胞产生并分泌。聚合免疫球蛋白受体，也称为pIgRs，在腺上皮的基底外侧表面表达，并介导分泌性免疫球蛋白聚合物跨上皮的转运。pIgR与分泌的二聚体IgA和IgM分子结合。在这些Ig聚合物跨细胞转运过程中，pIgR经历蛋白水解裂解，生成一个称为分泌成分（SC）、聚合免疫球蛋白受体或多聚IG受体的片段。当免疫球蛋白聚合物与SC结合时，它们在转运过程中变得能够抵抗酶降解。SC和pIgR对于黏膜免疫的正常功能至关重要，它们作为聚合Igs的分子伴侣，使其保持完整并进入体液。人类SC（pIgR）基因定位于1号染色体q31-q41，编码一个由764个氨基酸组成的蛋白质。该受体包含五个与免疫球蛋白的可变（V）区同源的单元和一个与某些免疫球蛋白可变区同源的跨膜区域。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SC 抗体 (C-2) 参考文献:

  1. 通过在转基因小鼠的乳腺上皮细胞中过度表达鼠聚合免疫球蛋白受体基因，提高牛奶中免疫球蛋白 A 的含量。  |  De Groot, N., et al. 2000. Immunology. 101: 218-24. PMID: 11012775
  2. 小鼠乳腺中的淋巴细胞归巢和 Ig 分泌。  |  van der Feltz, MJ., et al. 2001. Scand J Immunol. 54: 292-300. PMID: 11555393
  3. J 链对于聚合 Ig 受体介导的 IgA 上皮转运至关重要。  |  Johansen, FE., et al. 2001. J Immunol. 167: 5185-92. PMID: 11673531
  4. 母乳中的乳铁蛋白和游离分泌成分可抑制肠致病性大肠杆菌黏合到 HeLa 细胞上。  |  de Araújo, AN. and Giugliano, LG. 2001. BMC Microbiol. 1: 25. PMID: 11690544
  5. 编码人类跨膜分泌成分（多聚 Ig 受体）基因的分子克隆和外显子内含子图谱。  |  Krajci, P., et al. 1992. Eur J Immunol. 22: 2309-15. PMID: 1355431
  6. 聚合免疫球蛋白受体。  |  Aroeti, B., et al. 1992. Int Rev Cytol. 137B: 157-68. PMID: 1478818
  7. 禽类分泌成分的生物物理和生物化学特性为聚合Ig受体的进化提供了结构上的见解。  |  Stadtmueller, BM., et al. 2016. J Immunol. 197: 1408-14. PMID: 27412418
  8. 分泌成分（一种分泌性糖蛋白）在上皮细胞特异性吸收 IgA 二聚体中的作用。  |  Kühn, LC. and Kraehenbuhl, JP. 1979. J Biol Chem. 254: 11072-81. PMID: 387764
  9. T 细胞抗原受体和免疫球蛋白超基因家族。  |  Hood, L., et al. 1985. Cell. 40: 225-9. PMID: 3917857
  10. J链和分泌成分在人体免疫球蛋白受体介导的腺体和肝脏转运中的作用。  |  Brandtzaeg, P. 1985. Scand J Immunol. 22: 111-46. PMID: 3929375
  11. 免疫球蛋白缺乏症的分泌成分：肠上皮细胞的免疫电镜研究。  |  Nagura, H., et al. 1980. Scand J Immunol. 12: 359-63. PMID: 7466325
  12. 母体血清中免疫球蛋白A的分泌成分与早产预测。  |  Schellenberg, JC., et al. 1998. Am J Obstet Gynecol. 178: 535-9. PMID: 9539522

订购信息

产品名称产品编号规格价格数量收藏夹

SC 抗体 (C-2)

sc-374343
200 µg/ml
$316.00

SC (C-2): m-IgG Fc BP-HRP 套装

sc-529723
200 µg Ab; 10 µg BP
$354.00

SC (C-2): m-IgGκ BP-HRP 套装

sc-522705
200 µg Ab, 40 µg BP
$354.00

SC 抗体 (C-2) AC

sc-374343 AC
500 µg/ml, 25% agarose
$416.00

SC 抗体 (C-2) HRP

sc-374343 HRP
200 µg/ml
$316.00

SC 抗体 (C-2) FITC

sc-374343 FITC
200 µg/ml
$330.00

SC 抗体 (C-2) PE

sc-374343 PE
200 µg/ml
$343.00

SC 抗体 (C-2) Alexa Fluor® 488

sc-374343 AF488
200 µg/ml
$357.00

SC 抗体 (C-2) Alexa Fluor® 546

sc-374343 AF546
200 µg/ml
$357.00

SC 抗体 (C-2) Alexa Fluor® 594

sc-374343 AF594
200 µg/ml
$357.00

SC 抗体 (C-2) Alexa Fluor® 647

sc-374343 AF647
200 µg/ml
$357.00

SC 抗体 (C-2) Alexa Fluor® 680

sc-374343 AF680
200 µg/ml
$357.00

SC 抗体 (C-2) Alexa Fluor® 790

sc-374343 AF790
200 µg/ml
$357.00

HI Going through our fridge i have found several vials of AntiCD47(MIAP301) with different lot numbers. There were no dates written on the vials when they were received is there a data page with that info?

Asked by: SACoulter
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-07-28

I have used antibodies FH and SFN in past with good results. I was looking for using PIGR (sc-374343) for IHC experiments on FFPE. Do you think it will wok for it ? and can you share a protocol with me ?

Asked by: rmannan
Thank you for your question. Yes, this antibody, sc-374343 SC (C-2) is recommended for IHC on formalin fixed paraffin sections. You can find the recommended protocol here: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2020-01-03

SC Antibody (C-2): sc-374343. Is this antibody for succination of cysteine?

Asked by: hua ding
Thank you for your question. No, this antibody is recommend for detecting the secretory component (SC) fragment of polymeric immunoglobulin receptor.
Answered by: Technical Support
Date published: 2019-01-06

Can SC (C-2): sc-374343 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, SC (C-2): sc-374343 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 2 out of 5 by from Too much untargeted bandsThere are too many untargeted bands beyond the goal band.
Date published: 2017-09-21
Rated 4 out of 5 by from Good antigenicity1:500 dilution for WB, strip clear, responsive; 1:50 dilution for IF, clear fluorescence
Date published: 2017-03-09
Rated 5 out of 5 by from Got a good resutWe used it in our experiment for detection of SC of mouse, and got a good result.
Date published: 2016-12-02
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human urinary bladder tissue. -SCBT QC
Date published: 2015-02-13
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SC 抗体 (C-2) is rated 4.2 out of 5 by 5.
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