Date published: 2025-9-7

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SATB1 抗体 (C-6): sc-376096

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说明书
  • SATB1 抗体 C-6 是小鼠单克隆 IgG1 κ,SATB1抗体, 在10篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列241-310位于human物种的SATB1的内部区域内
  • SATB1 抗体 (C-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 SATB1
  • 抗SATB1抗体(C-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-376096 X, 200 µg/0.1 ml)
  • 关于如何获取SATB1 (C-6): sc-376096免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 SATB1 抗体 (C-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SATB1 抗体 (C-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

SATB1抗体(C-6)是一种IgG1 κ小鼠单克隆SATB1抗体(也称为特殊AT富集序列结合蛋白1(与核基质/支架相关DNA结合)抗体、DNA结合蛋白SATB1抗体或特殊AT富集序列结合蛋白1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的SATB1蛋白。SATB1抗体(C-6)既可作为非偶联抗SATB1抗体形式提供,也可作为多种偶联形式的抗SATB1抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。同源蛋白CCAAT位移蛋白(CDP)和特殊AT富集序列结合蛋白1(SATB1)是许多细胞基因的转录抑制因子,它们参与细胞发育和细胞类型分化。SATB1主要在胸腺细胞中表达,与CDP一样,它还包含一个独特的同源框DNA结合域,这对DNA结合至关重要。SATB1和CDP通过这些同源域相互作用,并协同作为基因表达的介质。SATB1包含一个额外的结构域,该结构域对DNA具有更高的亲和力,并专门促进SATB1与DNA的核基质附着区(MARs)之间的直接关联。MARs是特定的DNA序列,与核基质结合并形成染色体环的基底,这些环在细胞核内组织染色体并调节DNA转录和复制。SATB1与MARs的碱基非配对区域内的核心解旋元件的结合需要SATB1的MAR和同源框结合域。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SATB1 抗体 (C-6) 参考文献:

  1. 同源蛋白 CDP 和 SATB1 相互作用:组织特异性调控的潜力。  |  Liu, J., et al. 1999. Mol Cell Biol. 19: 4918-26. PMID: 10373541
  2. 基质附着区蛋白 SATB1 与 BCL2 主要断点区侧翼富含 AT 的序列的结合调节。  |  Ramakrishnan, M., et al. 2000. Mol Cell Biol. 20: 868-77. PMID: 10629043
  3. MAR结合蛋白SATB1在T细胞发育过程中协调多个基因的时空表达。  |  Alvarez, JD., et al. 2000. Genes Dev. 14: 521-35. PMID: 10716941
  4. 特殊富 AT 序列结合蛋白 1 (SATB1) 在碱基切除修复中发挥辅助因子的功能  |  Kaur, S., et al. 2016. J Biol Chem. 291: 22769-22780. PMID: 27590341
  5. 特殊富AT序列结合蛋白1(SATB1)及其在实体瘤中的作用。  |  Frömberg, A., et al. 2018. Cancer Lett. 417: 96-111. PMID: 29306014
  6. 特殊富 AT 序列结合蛋白 1 (SATB1) 与乳腺癌、头颈癌和前列腺癌中的免疫渗透有关。  |  Ge, H., et al. 2020. Med Sci Monit. 26: e923208. PMID: 32562536
  7. 微RNA-21介导的SATB1/S100A9/NF-κB轴促进了慢性阻塞性肺病的发病机制。  |  Kim, RY., et al. 2021. Sci Transl Med. 13: eaav7223. PMID: 34818056
  8. TGF-β 介导的基因组组织者 SATB1 沉默可促进 Tfh 细胞分化和瘤内三级淋巴结构的形成。  |  Chaurio, RA., et al. 2022. Immunity. 55: 115-128.e9. PMID: 35021053
  9. SATB1 中的一个非典型同源结构域促进了对基质附着区域中关键结构元素的特异性识别。  |  Dickinson, LA., et al. 1997. J Biol Chem. 272: 11463-70. PMID: 9111059
  10. 核基质相关区域(MAR)结合蛋白 SATB1 和 CDP/Cux 与小鼠 CD8a 基因上游调控区域中的 MAR 元素(L2a)的相互作用。  |  Banan, M., et al. 1997. J Biol Chem. 272: 18440-52. PMID: 9218488
  11. 在 Jurkat T 细胞中,与特殊富 AT 序列结合蛋白 1(SATB1)结合的基因组序列与染色质环基部的核基质紧密相连。  |  de Belle, I., et al. 1998. J Cell Biol. 141: 335-48. PMID: 9548713
  12. T 细胞受体 beta 基因增强子上游的核基质附着区与 Cux/CDP 和 SATB1 结合,并调节增强子依赖性报告基因的表达,但不调节内源基因的表达。  |  Chattopadhyay, S., et al. 1998. J Biol Chem. 273: 29838-46. PMID: 9792700

订购信息

产品名称产品编号规格价格数量收藏夹

SATB1 抗体 (C-6)

sc-376096
200 µg/ml
$316.00

SATB1 (C-6): m-IgG Fc BP-HRP 套装

sc-527512
200 µg Ab; 10 µg BP
$354.00

SATB1 (C-6): m-IgG1 BP-HRP 套装

sc-532885
200 µg Ab; 20 µg BP
$354.00

SATB1 抗体 (C-6) X

sc-376096 X
200 µg/0.1 ml
$316.00

SATB1 抗体 (C-6) AC

sc-376096 AC
500 µg/ml, 25% agarose
$416.00

SATB1 抗体 (C-6) HRP

sc-376096 HRP
200 µg/ml
$316.00

SATB1 抗体 (C-6) FITC

sc-376096 FITC
200 µg/ml
$330.00

SATB1 抗体 (C-6) PE

sc-376096 PE
200 µg/ml
$343.00

SATB1 抗体 (C-6) Alexa Fluor® 488

sc-376096 AF488
200 µg/ml
$357.00

SATB1 抗体 (C-6) Alexa Fluor® 546

sc-376096 AF546
200 µg/ml
$357.00

SATB1 抗体 (C-6) Alexa Fluor® 594

sc-376096 AF594
200 µg/ml
$357.00

SATB1 抗体 (C-6) Alexa Fluor® 647

sc-376096 AF647
200 µg/ml
$357.00

SATB1 抗体 (C-6) Alexa Fluor® 680

sc-376096 AF680
200 µg/ml
$357.00

SATB1 抗体 (C-6) Alexa Fluor® 790

sc-376096 AF790
200 µg/ml
$357.00

Which site did SATB1 抗体 (N-14): sc-5989 antibody recognize?No anwser here. I need this information for my science paper, can you tell me?

Asked by: abboy119
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-04-29

Which site did this antibody recognize?No anwser here. I need this information for my science paper, can you tell me?

Asked by: abboy119
Thank you for your question. As indicated in the product description above, Anti-SATB1 Antibody (C-6): sc-376096 was raised against amino acids 241-310 mapping within an internal region of SATB1 of human origin.
Answered by: Tech Support Europe
Date published: 2022-04-28

I am using SATB1 (C-6): sc-376096 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Satb1 in XenopusI have tested this antibody in IF with sections fixed in PAF4% at a concentration of 1:500 and it works very well in Xenopus laevis.
Date published: 2023-02-28
Rated 1 out of 5 by from Weak antibodyNot working at 1:100 WB in HCT116 whole cell lysate.
Date published: 2018-09-03
Rated 5 out of 5 by from Excellent on spinal cord tissuesWe used this antibody on frozen sections from monkey and rat spinal cord tissue. Great at 1:200 using fluorescent secondaries. No antigen retrieval necessary with our paraformaldehyde fixed tissue.
Date published: 2018-03-28
Rated 5 out of 5 by from Works great on embryonic tissueWorks great on embryonic tissue sliced via cryosectioning. Citrate buffer antigen retrieval enhanced staining in samples fixed for longer periods of time (2hrs and above).
Date published: 2017-02-09
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of SATB1 expression in Jurkat, MOLT-4 and CTLL-2 whole cell lysates.
Date published: 2017-01-26
Rated 5 out of 5 by from Produced exceptional immunoperoxidase nuclearProduced exceptional immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human fetal thymus tissue. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Satisfactory Western Blot data of SATB1 expressionSatisfactory Western Blot data of SATB1 expression in non-transfected and mouse SATB1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-12-14
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SATB1 抗体 (C-6) is rated 4.4 out of 5 by 7.
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