Date published: 2025-9-7
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RUNX1 Antibody (A-2) - Western Blotting - Image 354385
RUNX1 抗体 (A-2): sc-365644. HeLa 核提取物 (A), NRK (B) 和 A-10 (C) 全细胞裂解液中 RUNX1 表达的 Western 印迹分析.
RUNX1 抗体 (A-2): sc-365644. HeLa 核提取物 (A), NRK (B) 和 A-10 (C) 全细胞裂解液中 RUNX1 表达的 Western 印迹分析.

RUNX1 抗体 (A-2): sc-365644

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说明书
  • RUNX1 抗体 A-2 是小鼠单克隆 IgG1 κ，RUNX1抗体, 在54篇文献中引用，规格为200 µg/ml
  • 针对human起源的RUNX1内部区域内的氨基酸186-250进行
  • RUNX1 抗体 (A-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 a broad range of RUNX1 isoforms
  • 抗RUNX1抗体（A-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-365644 X, 200 µg/0.1 ml)
  • 关于如何获取RUNX1 (A-2): sc-365644的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 RUNX1 抗体 (A-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RUNX1 抗体 (A-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 RUNX 抗体 包括 RUNX1, RUNX2和RUNX3

RUNX1抗体（A-2）是一种IgG1 κ小鼠单克隆RUNX1抗体（也称为AML1抗体或CBFA2抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的RUNX1蛋白。RUNX1抗体（A-2）既可以是非偶联的抗RUNX1抗体形式，也可以是多种偶联形式的抗RUNX1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。哺乳动物Runt相关转录因子（RUNX）家族包括三个成员，RUNX1（也称为AML-1、PEBP2αB、CBFA2）、RUNX2（也称为AML-3、PEBP2αA、CBFA1、Osf2）和RUNX3（也称为AML-2、PEBP2αC、CBFA3）。RUNX家族成员是DNA结合蛋白，可调节参与细胞分化和细胞周期进程的基因的表达。RUNX1参与造血，并经常通过染色体易位成为人类白血病的靶标，这种染色体易位将RUNX1的DNA结合结构域与其他转录因子和辅阻遏物分子融合。除了其在白血病发生中的作用外，RUNX1还参与感觉神经元的多样化。具体来说，RUNX1促进轴突生长，在神经嵴衍生的TrkA+感觉神经元中选择性表达，并介导迁移神经嵴细胞中的TrkA反式激活。选择性剪接产生几种RUNX1的同种型。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RUNX1 抗体 (A-2) 参考文献:

  1. RUNX 基因家族的致癌潜力：'概述'。  |  Ito, Y. 2004. Oncogene. 23: 4198-208. PMID: 15156173
  2. RUNX 家族成员在转录抑制和基因沉默中的作用  |  Durst, KL. and Hiebert, SW. 2004. Oncogene. 23: 4220-4. PMID: 15156176
  3. 白血病/果蝇同源性。  |  Daga, A., et al. 1992. Nature. 356: 484. PMID: 1560822
  4. Runx 蛋白在软骨形成过程中的作用  |  Yoshida, CA. and Komori, T. 2005. Crit Rev Eukaryot Gene Expr. 15: 243-54. PMID: 16390320
  5. Runx1/AML1转录因子选择性地调节TrkA痛觉神经元的发育和存活。  |  Marmigère, F., et al. 2006. Nat Neurosci. 9: 180-7. PMID: 16429136
  6. Runx转录因子信号在背根神经节感觉神经元多样化中的作用  |  Kramer, I., et al. 2006. Neuron. 49: 379-93. PMID: 16446142
  7. Runx1在血细胞发育中的结构和功能。  |  Bonifer, C., et al. 2017. Adv Exp Med Biol. 962: 65-81. PMID: 28299651
  8. 组蛋白去乙酰化酶在急性白血病中的作用  |  Fenrick, R. and Hiebert, SW. 1998. J Cell Biochem. 72 Suppl 30-31: 194-202. PMID: 29345812
  9. 与急性髓性白血病有关的 AML1 基因的替代剪接和基因组结构。  |  Miyoshi, H., et al. 1995. Nucleic Acids Res. 23: 2762-9. PMID: 7651838
  10. 急性淋巴细胞白血病中 12p13 上的 TEL 基因与 21q22 上的 AML1 基因融合。  |  Golub, TR., et al. 1995. Proc Natl Acad Sci U S A. 92: 4917-21. PMID: 7761424
  11. 人类急性髓性白血病基因 AML1 可编码多种不同剪接和不同表达的 mRNA。  |  Levanon, D., et al. 1996. DNA Cell Biol. 15: 175-85. PMID: 8634147
  12. 染色体易位导致的白血病发生  |  Mitani, K. 1997. Leukemia. 11 Suppl 3: 294-6. PMID: 9209370

订购信息

产品名称产品编号规格价格数量收藏夹

RUNX1 抗体 (A-2)

sc-365644
200 µg/ml
$316.00

RUNX1 (A-2): m-IgG Fc BP-HRP 套装

sc-529421
200 µg Ab; 10 µg BP
$354.00

RUNX1 (A-2): m-IgGκ BP-HRP 套装

sc-522308
200 µg Ab, 40 µg BP
$354.00

RUNX1 (A-2): m-IgG1 BP-HRP 套装

sc-543507
200 µg Ab; 20 µg BP
$354.00

RUNX1 抗体 (A-2) X

sc-365644 X
200 µg/0.1 ml
$316.00

RUNX1 抗体 (A-2) AC

sc-365644 AC
500 µg/ml, 25% agarose
$416.00

RUNX1 抗体 (A-2) HRP

sc-365644 HRP
200 µg/ml
$316.00

RUNX1 抗体 (A-2) FITC

sc-365644 FITC
200 µg/ml
$330.00

RUNX1 抗体 (A-2) PE

sc-365644 PE
200 µg/ml
$343.00

RUNX1 抗体 (A-2) Alexa Fluor® 488

sc-365644 AF488
200 µg/ml
$357.00

RUNX1 抗体 (A-2) Alexa Fluor® 546

sc-365644 AF546
200 µg/ml
$357.00

RUNX1 抗体 (A-2) Alexa Fluor® 594

sc-365644 AF594
200 µg/ml
$357.00

RUNX1 抗体 (A-2) Alexa Fluor® 647

sc-365644 AF647
200 µg/ml
$357.00

RUNX1 抗体 (A-2) Alexa Fluor® 680

sc-365644 AF680
200 µg/ml
$357.00

RUNX1 抗体 (A-2) Alexa Fluor® 790

sc-365644 AF790
200 µg/ml
$357.00

Hi, I need RUNX1 FITC conjugated antibody against human specificity. I am mainly focus on flow cytometry. The catalog no SC 365644 FITC can be used for flow cytometry. 

Asked by: Preetha
Thank you for your question. sc-365644 FITC can be used for Flow Cytometry. We recommend the antibody for WB, IP, PAR,and ELISA. Here is our Flow Cytometry protocol : https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2025-04-04

Is this region present in AML1-ETO and TEL-AML1? Does this antibody cross-react with them?

Asked by: zaizaicheng
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2023-10-19

Hello! What is the principle difference between sc-365644 and sc-365644 X? Is sc-365644 X validated for ChIP?

Asked by: Sveta K
Thank you for your question the TransCruz Reagent, sc-365644 X, is 10X more concentrated than the standard formulation of the antibody, in a buffer of PBS + azide (without gelatin). We are aware of one publication citing use of this antibody for ChIP. Please refer to PMID: 29779944 for more details.
Answered by: Technical Support
Date published: 2019-01-06

Is this region present in RUNX2 and RUNX3? Does this antibody cross-react with RUNX2 and 3?

Asked by: BrianE
Thank you for your question. This antibody does not have any cross-reactivity to RUNX2 and RUNX3.
Answered by: Tech Service
Date published: 2021-07-15

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with RUNX1 Antibody (A-2): sc-365644?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 4 out of 5 by from funzionaQuesto anticorpo funziona bene. Io l'ho usato diluito 1:250 con smascheramento antigenico in pH9.0 (microwave). Vede le cellule linfoidi
Date published: 2025-06-09
Rated 5 out of 5 by from Good results for IF in frozen sectionsGood results with 1:50 dilution in 30 minute 4% PFA fixed cryosections of mammalian retina (mk, ch, gp, rb).
Date published: 2024-12-06
Rated 5 out of 5 by from Specificity and consistencyI bought the antibody few months ago and tried 2 times as a part of optimization and now on will use more. The antibody gave consistent bands with 1:500 and 1:1000 dilution without non-specifc bands. It binds to isoform a at 28kDa and isoform b and c at 45-50Kda.
Date published: 2023-01-14
Rated 4 out of 5 by from Great antibodyI have successfully used RUNX1 to stain mouse xenograft of human gastric cancer MKN45 cell line, which I knew to be positive. Plus, it worked also well when staining human gastric carcinoma samples by IHC (PMID: 30662000).
Date published: 2020-06-23
Rated 5 out of 5 by from Anticuerpo excelente para IHCEste anticuerpo detecta específicamente RUNX1 en núcleos de tejidos parafinados y en células fijadas con formaldehído. Muy buen anticuerpo.
Date published: 2018-02-07
Rated 5 out of 5 by from IHC/PWork well in the IHC(P) for tibia of mouse (1:100)
Date published: 2017-10-23
Rated 5 out of 5 by from good qualityI used RUNX1 antibody to test HeLa nuclear extract for purity, I have used the antibody for western blotting (1:400) and in for immunofluorescence . It work very well and is very sensitive for western blot . IF Staining also looks correct.
Date published: 2017-08-18
Rated 4 out of 5 by from Good antibodyThe antibody works great for westernblotting using mouse brain tissue at 1:500 dilution.
Date published: 2017-07-24
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RUNX1 抗体 (A-2) is rated 4.9 out of 5 by 20.
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