Date published: 2025-9-6
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RIG-I Antibody (D-12) - Western Blotting - Image 352707
RIG-I 抗体 (D-12): sc-376845. Jurkat (A), THP-1 (B), HUV-EC-C (C) 和 A-431 (D) 全细胞裂解液中 RIG-I 表达的 Western 印迹分析.
RIG-I 抗体 (D-12): sc-376845. Jurkat (A), THP-1 (B), HUV-EC-C (C) 和 A-431 (D) 全细胞裂解液中 RIG-I 表达的 Western 印迹分析.

RIG-I 抗体 (D-12): sc-376845

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说明书
  • RIG-I 抗体 D-12 是小鼠单克隆 IgG1 κ，RIG-I抗体, 在43篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列5-300位于mouse物种的RIG-I的N-terminus
  • RIG-I 抗体 (D-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 RIG-I
  • 抗RIG-I抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RIG-I (D-12): sc-376845的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成RIG-I 抗体 (D-12)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

RIG-I抗体（D-12）是一种IgG1 κ小鼠单克隆RIG-I抗体（也称为DDX58抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的RIG-I蛋白。RIG-I抗体（D-12）既可以是非偶联的抗RIG-I抗体形式，也可以是多种偶联形式的抗RIG-I抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。先天免疫系统通过识别许多不同的病毒成分并触发特定的抗病毒反应来感知病毒感染。细胞内双链RNA（dsRNA）是许多病毒复制的主要标志。视黄酸诱导基因I（RIG-I）是一种925个氨基酸的干扰素诱导型细胞DExD/H盒RNA解旋酶，可激活I型干扰素（IFN），这是先天免疫系统对这些dsRNA病毒敏感的重要效应器。dsRNA在细胞中的含量通常很低，因此当病毒存在时，dsRNA水平升高会作为信号告诉RIG-I激活IFN的产生。RIG-I通过其解旋酶结构域与病毒dsRNA结合来实现这一点，从而通过IκB激酶相关激酶传递IFN的激活信号并诱导IFN表达。RIG-I表达于成纤维细胞和常规树突状细胞的细胞质中，并能区分许多不同的RNA病毒。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RIG-I 抗体 (D-12) 参考文献:

  1. 通过细胞 RNA 螺旋酶 RIG-I 调节细胞内的抗病毒防御和对丙型肝炎病毒 RNA 复制的允许性。  |  Sumpter, R., et al. 2005. J Virol. 79: 2689-99. PMID: 15708988
  2. RIG-I：病毒诱导干扰素产生的重要调节器。  |  Heim, MH. 2005. J Hepatol. 42: 431-3. PMID: 15710232
  3. 在丙型肝炎病毒表达过程中抑制 RIG-I 依赖性信号传导至干扰素通路，并通过 IKKepsilon 恢复信号传导。  |  Breiman, A., et al. 2005. J Virol. 79: 3969-78. PMID: 15767399
  4. 细胞类型特异性的 RIG-I 参与抗病毒反应。  |  Kato, H., et al. 2005. Immunity. 23: 19-28. PMID: 16039576
  5. 甲型肝炎病毒抑制 RIG-I 介导的 IRF-3 激活，从而阻止了 beta 干扰素的诱导。  |  Fensterl, V., et al. 2005. J Virol. 79: 10968-77. PMID: 16103148
  6. [RIG-I参与抗病毒反应的细胞类型特异性]。  |  Kato, H., et al. 2006. Nihon Rinsho. 64: 1244-7. PMID: 16838639
  7. 激活 RIG-I 通路后诱导 IRF-3 和 IRF-7 磷酸化。  |  Paz, S., et al. 2006. Cell Mol Biol (Noisy-le-grand). 52: 17-28. PMID: 16914100
  8. EB 病毒编码的 RNA 会被 RIG-I 识别，并激活诱导 I 型 IFN 的信号。  |  Samanta, M., et al. 2006. EMBO J. 25: 4207-14. PMID: 16946700
  9. RIG-I样受体的免疫信号传导。  |  Loo, YM. and Gale, M. 2011. Immunity. 34: 680-92. PMID: 21616437
  10. RIG-I激活和信号传导的分子机制。  |  Thoresen, D., et al. 2021. Immunol Rev. 304: 154-168. PMID: 34514601
  11. 利用 RIG-I 样受体途径进行癌症免疫疗法。  |  Jiang, Y., et al. 2023. J Hematol Oncol. 16: 8. PMID: 36755342
  12. 补充NAD+前体可防止肾脏损伤期间mtRNA/RIG-I依赖性炎症。  |  Doke, T., et al. 2023. Nat Metab. 5: 414-430. PMID: 36914909

订购信息

产品名称产品编号规格价格数量收藏夹

RIG-I 抗体 (D-12)

sc-376845
200 µg/ml
$316.00

RIG-I 抗体 (D-12) AC

sc-376845 AC
500 µg/ml, 25% agarose
$416.00

RIG-I 抗体 (D-12) HRP

sc-376845 HRP
200 µg/ml
$316.00

RIG-I 抗体 (D-12) FITC

sc-376845 FITC
200 µg/ml
$330.00

RIG-I 抗体 (D-12) PE

sc-376845 PE
200 µg/ml
$343.00

RIG-I 抗体 (D-12) Alexa Fluor® 488

sc-376845 AF488
200 µg/ml
$357.00

RIG-I 抗体 (D-12) Alexa Fluor® 546

sc-376845 AF546
200 µg/ml
$357.00

RIG-I 抗体 (D-12) Alexa Fluor® 594

sc-376845 AF594
200 µg/ml
$357.00

RIG-I 抗体 (D-12) Alexa Fluor® 647

sc-376845 AF647
200 µg/ml
$357.00

RIG-I 抗体 (D-12) Alexa Fluor® 680

sc-376845 AF680
200 µg/ml
$357.00

RIG-I 抗体 (D-12) Alexa Fluor® 790

sc-376845 AF790
200 µg/ml
$357.00

Has this been used in immunofluorescence? Would like to try to use in IF

Asked by: lpthedon
Anti-RIG-I Antibody (D-12) is available conjugated to phycoerythrin or FITC for IF, IHC(P) and FCM.
Answered by: Technical Service
Date published: 2024-03-28

WAS KNOCK DOWN VALIDATION DONE FOR THIS CLONE OF RIG I A.

Asked by: Priyanka m
Thank you for your question. We have not yet performed any RIG-I knockdown experiments to confirm the specificity of this monoclonal antibody in a knockdown or knockout sample. We have extensive Western blot data on our website showing representative data with this antibody in different cell lines, as well as at least 4 known product citations with this antibody.
Answered by: Technical Support
Date published: 2019-04-13

Hi. I would like to use this antibody to study RIG-I expression in mice or murine cell lines by flow cytometry. Did you ever test it by flow cytometry? Did any research paper use it before for flow cytometry? Thanks a lot for answering

Asked by: Nour
Thank you for your question. Unfortunately we do not have any papers using this in Flow cytometry, and we have not tested this application in house. However, we would be glad to send you a sample of it to try, because it is definitely possible that it works great for Flow Cytometry. If you would like to try a sample, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-20

Dear Sir/Madam, I would like to purchase the product sc-376845 HRP but to do so I would need a price quote from you in order to pass it on to finance dep.. Many thanks, Marios

Asked by: Marios
Thank you for your question. To obtain a quote for our, RIG-I Antibody (D-12) conjugated to HRP, sc-376845 HRP, please contact our European Support office to request a quote with the more accurate information for your region. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-03-24

I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2017-01-27
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Rated 5 out of 5 by from Works well!Great antibody, this works well with the target that we are looking for in the lab.
Date published: 2025-06-26
Rated 4 out of 5 by from good antibodyWe tested this antibody by western blot and it gives a clean band.
Date published: 2019-11-27
Rated 4 out of 5 by from Worked wonderfully!Our newest result with RIG-I antibody from SC (sc-376845) in mouse cell lines!
Date published: 2018-08-07
Rated 4 out of 5 by from Works GoodI received the sample Ab 2 weeks ago, after testing the Ab could detect RIG-I correctly at 108KDa, though there are some non-specific bands under 70 kDa. The optimization still undergoing. If it goes well, I will consider to buy full size product.
Date published: 2018-02-08
Rated 5 out of 5 by from Worked really wellUsed at 1:1000 dilution in PBST to detect RIG-I in uninduced A549 and HaCaT cell lines, gave very clean blot
Date published: 2017-10-30
Rated 5 out of 5 by from Works good in WBThis Ab with 1:1000 dilution was used for the detection of human samples and the interested band could be observed clearly in WB image.
Date published: 2017-09-28
Rated 5 out of 5 by from great antibodyVirus infected A549 cell for 24 hours with different dose, I detected distinct bands
Date published: 2017-09-14
Rated 5 out of 5 by from right bandThis antibody shows the right band.The dilution is used 1:600,the result is very statisfuied for us.
Date published: 2017-09-02
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RIG-I 抗体 (D-12) is rated 4.6 out of 5 by 17.
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