Date published: 2025-9-7
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Rictor Antibody (H-11) - Immunofluorescence - Image 370933
Rictor 抗体 (H-11): sc-271081. 福尔马林固定的 HeLa 细胞免疫荧光染色, 显示细胞质小泡定位.
Rictor 抗体 (H-11): sc-271081. 福尔马林固定的 HeLa 细胞免疫荧光染色, 显示细胞质小泡定位.

Rictor 抗体 (H-11): sc-271081

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说明书
  • Rictor 抗体 H-11 是小鼠单克隆 IgG2b κ，Rictor抗体, 在29篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列20-297位于human物种的Rictor的N-terminus附近
  • Rictor 抗体 (H-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Rictor; 也和以下物种反应，包括： canine
  • 抗Rictor抗体（H-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rictor (H-11): sc-271081的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 是Rictor 抗体 (H-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Rictor 抗体 (H-11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Rictor抗体（H-11）是一种IgG2b κ小鼠单克隆Rictor抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类的Rictor蛋白。Rictor抗体（H-11）既可以是非偶联的抗Rictor抗体形式，也可以是多种偶联形式的抗Rictor抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。FRAP是一种大蛋白激酶，是雷帕霉素的哺乳动物靶点，雷帕霉素是一种免疫抑制剂，可阻止血管再狭窄，并具有潜在的抗癌作用。 FRAP的雷帕霉素不敏感伴侣，也称为Rictor，与D. discoidieum的pianissimo、S. pombe的STE20p和S. cerevisiae的AVO3p具有同源性。Rictor与FRAP形成复合物，这在细胞生长调控中很重要，因为它整合了生长因子和营养衍生信号。Rictor-FRAP复合物在PKCα磷酸化中发挥作用，直接在体外Ser473上磷酸化Akt/PKB，并通过PDK1促进Thr308磷酸化。它还可能作为已丧失PTEN表达的肿瘤的药物靶点，PTEN是一种反对Akt/PKB激活的肿瘤抑制因子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Rictor 抗体 (H-11)

sc-271081
200 µg/ml
$316.00

Rictor (H-11): m-IgG2b BP-HRP 套装

sc-548692
200 µg Ab; 10 µg BP
$354.00

Rictor 抗体 (H-11) AC

sc-271081 AC
500 µg/ml, 25% agarose
$416.00

Rictor 抗体 (H-11) HRP

sc-271081 HRP
200 µg/ml
$316.00

Rictor 抗体 (H-11) FITC

sc-271081 FITC
200 µg/ml
$330.00

Rictor 抗体 (H-11) PE

sc-271081 PE
200 µg/ml
$343.00

Rictor 抗体 (H-11) Alexa Fluor® 488

sc-271081 AF488
200 µg/ml
$357.00

Rictor 抗体 (H-11) Alexa Fluor® 546

sc-271081 AF546
200 µg/ml
$357.00

Rictor 抗体 (H-11) Alexa Fluor® 594

sc-271081 AF594
200 µg/ml
$357.00

Rictor 抗体 (H-11) Alexa Fluor® 647

sc-271081 AF647
200 µg/ml
$357.00

Rictor 抗体 (H-11) Alexa Fluor® 680

sc-271081 AF680
200 µg/ml
$357.00

Rictor 抗体 (H-11) Alexa Fluor® 790

sc-271081 AF790
200 µg/ml
$357.00

What do you recommend for Antibody dilution? Nonfat Milk or BSA?

Asked by: Swoj
Thank you for your question. The recommended starting dilution for Western blotting is 1:200 (range 1:100-1:1,000). We recommend using 5% nonfat milk in TBST as blocking buffer, and antibody diluent. Please refer to our protocol online for further details.
Answered by: Technical Support
Date published: 2019-09-23

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Rictor (H-11): sc-271081?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-22
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Rated 5 out of 5 by from great antibodyI tested this antibody for western blot using three different cell lines, got a strong signal on as little as 10 ug of total protein.
Date published: 2021-10-15
Rated 5 out of 5 by from Excellent antibodyExcellent antibody and excellent WB. In 3% milk / TBST
Date published: 2021-03-17
Rated 1 out of 5 by from Anticorpo non performante.Abbiamo testato questo anticorpo su tonsilla umana e linfonodo reattivo alla condizione 1:50 ph6 e ph9.
Date published: 2021-03-09
Rated 4 out of 5 by from good!I tested both rictor antibodies (#271081 and #81538) on Western blot and they were quite specific
Date published: 2020-09-07
Rated 4 out of 5 by from good for western1:1000 dilution in 3% BSA in PBST worked for western blotting of samples from mouse tissues and cells. Two bands were detected. It is likely the upper band is the right size.
Date published: 2017-11-29
Rated 5 out of 5 by from Good FeaturesI recommended for detection of Rictor of mouse, rat and human origin by WB
Date published: 2017-11-09
Rated 4 out of 5 by from Its function is very good.I applied for this product sample sack and conducted western blot by using human kenay cancer cell, and found that it worked well.
Date published: 2017-07-11
Rated 5 out of 5 by from Good stainingI used for staining mouse intestine tissue and it worked great (1:100)
Date published: 2017-06-12
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Rictor 抗体 (H-11) is rated 4.6 out of 5 by 18.
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