Date published: 2025-9-6

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Rho G 抗体 (1F3 B3 E5): sc-80015

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说明书
  • Rho G 抗体 1F3 B3 E5 是小鼠单克隆 IgG2a κ,Rho G抗体, 在11篇文献中引用,规格为200 µg/ml
  • 针对mouse来源的C-terminal Rho G肽
  • Rho G 抗体 (1F3 B3 E5) 推荐用于 WB 和 IP,检测mouse, rat 和human 来源的 endogenous Rho G
  • 抗Rho G抗体(1F3 B3 E5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rho G (1F3 B3 E5): sc-80015免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Rho G Antibody (1F3 B3 E5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与Rho G Antibody (1F3 B3 E5) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

Rho G抗体(1F3 B3 E5)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)和免疫沉淀(IP)检测小鼠、大鼠和人类来源的Rho G蛋白。抗Rho G抗体(1F 3 B3 E5)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Rho G蛋白在调节肌动蛋白细胞骨架方面发挥着关键作用,而肌动蛋白细胞骨架对于细胞迁移、粘附和分裂等各种细胞过程至关重要。Rho G的正常功能对于维持细胞结构和促进细胞间交流至关重要,因此Rho G是发育过程和组织稳态的关键参与者。Rho G失调与多种病理状况有关,包括癌症转移和心血管疾病,这凸显了在基础和应用研究中研究Rho G的重要性。Rho G蛋白与Ras超家族的其他成员一样,含有羧基末端CAAX结构域,该结构域对于翻译后修饰和膜定位至关重要,进一步强调了Rho G在细胞信号通路中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rho G 抗体 (1F3 B3 E5) 参考文献:

  1. p21rho 蛋白的翻译后修饰。  |  Adamson, P., et al. 1992. J Biol Chem. 267: 20033-8. PMID: 1400319
  2. RhoG 在 ICAM1 参与的下游调节内皮顶杯的组装,并参与白细胞跨内皮迁移。  |  van Buul, JD., et al. 2007. J Cell Biol. 178: 1279-93. PMID: 17875742
  3. Ephexin4 和 EphA2 通过 RhoG 依赖性机制介导细胞迁移。  |  Hiramoto-Yamaki, N., et al. 2010. J Cell Biol. 190: 461-77. PMID: 20679435
  4. 成年小鼠组织中与 ras 相关的 ralA, rho12 和 rab 基因的表达。  |  Olofsson, B., et al. 1988. Oncogene. 3: 231-4. PMID: 2457862
  5. 鉴定 ras/R-ras 和 rho 调控蛋白的不同细胞质靶标。  |  Garrett, MD., et al. 1989. J Biol Chem. 264: 10-3. PMID: 2491843
  6. 在没有磷酸肌醇水解的情况下,用 ras 蛋白对瑞士 3T3 细胞进行刮擦加载可迅速激活蛋白激酶 C。  |  Morris, JD., et al. 1989. Oncogene. 4: 27-31. PMID: 2536916
  7. SGEF 的酪氨酸磷酸化调节 RhoG 活性和细胞迁移。  |  Okuyama, Y., et al. 2016. PLoS One. 11: e0159617. PMID: 27437949
  8. Tiam1、RhoG和ELMO2/ILK参与人类小梁网细胞中Rac1介导的吞噬作用。  |  Peotter, JL., et al. 2016. Exp Cell Res. 347: 301-11. PMID: 27539661
  9. ras 超家族蛋白。  |  Chardin, P. 1988. Biochimie. 70: 865-8. PMID: 3145021
  10. ras 基因。  |  Barbacid, M. 1987. Annu Rev Biochem. 56: 779-827. PMID: 3304147
  11. 人类 rho cDNA 克隆 12 的核苷酸序列。  |  Yeramian, P., et al. 1987. Nucleic Acids Res. 15: 1869. PMID: 3822842
  12. 一个新的ras相关基因家族  |  Madaule, P. and Axel, R. 1985. Cell. 41: 31-40. PMID: 3888408

订购信息

产品名称产品编号规格价格数量收藏夹

Rho G 抗体 (1F3 B3 E5)

sc-80015
200 µg/ml
$316.00

Rho G (1F3 B3 E5): m-IgGκ BP-HRP 套装

sc-521155
200 µg Ab, 40 µg BP
$354.00

Rho G 抗体 (1F3 B3 E5) AC

sc-80015 AC
500 µg/ml, 25% agarose
$416.00

Rho G 抗体 (1F3 B3 E5) HRP

sc-80015 HRP
200 µg/ml
$316.00

Rho G 抗体 (1F3 B3 E5) FITC

sc-80015 FITC
200 µg/ml
$330.00

Rho G 抗体 (1F3 B3 E5) PE

sc-80015 PE
200 µg/ml
$343.00

Rho G 抗体 (1F3 B3 E5) Alexa Fluor® 488

sc-80015 AF488
200 µg/ml
$357.00

Rho G 抗体 (1F3 B3 E5) Alexa Fluor® 546

sc-80015 AF546
200 µg/ml
$357.00

Rho G 抗体 (1F3 B3 E5) Alexa Fluor® 594

sc-80015 AF594
200 µg/ml
$357.00

Rho G 抗体 (1F3 B3 E5) Alexa Fluor® 647

sc-80015 AF647
200 µg/ml
$357.00

Rho G 抗体 (1F3 B3 E5) Alexa Fluor® 680

sc-80015 AF680
200 µg/ml
$357.00

Rho G 抗体 (1F3 B3 E5) Alexa Fluor® 790

sc-80015 AF790
200 µg/ml
$357.00

Are there any issues with using sc-80015: Rho G (1F3 B3 E5) mouse monoclonal primary antibody on mouse samples?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-80015 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from 功能很出色之前购买了此产品,用于WB实验,效果还不错,实验结果显示的条带很清晰,实验结果显示的条带很清晰,实验结果显示的条带很清晰,
Date published: 2019-05-15
Rated 5 out of 5 by from Rho GExcelent antibody for western blot. Detected Rho G at expected size in 15 ug total proteine lysate, wet transfer one hour at 450 mAmps on PVDF membrane, overnight blocking with 5% nonfat dry milk in PBS, one hour incubation at RT with 1:500 anti RhoG in 5% PBS with 0,2%Tween followed by one hour incubation with 1:10.000 anti mouse (sc-358914) HRP conjugated antibody and detection with ECL prime substrate followed by short film exposure (under 1 minute) and automatic processing.
Date published: 2017-09-25
Rated 5 out of 5 by from Great for MDA-MB-231 and A549Clean specific bands at the expected kDa, the results were great, and what we expected!
Date published: 2017-07-06
Rated 5 out of 5 by from anti-RhoGGreat antibody for WB. Have tested in different cell lines and species (human, mouse, rat, dog). Very clean. Does not recognize Rac or other GTPases.
Date published: 2017-06-23
Rated 5 out of 5 by from Good WB antibody in MDAGood WB antibody in MDA-MB-231, MCK7 and Rat2 whole cell lysates. -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Strong band in WB in human HeLa cellsStrong band in WB in human HeLa cells. -SCBT Publication Review
Date published: 2015-02-19
Rated 5 out of 5 by from Good specificity in WB in mouse 293T wholeGood specificity in WB in mouse 293T whole cell lysates. -SCBT QC
Date published: 2015-01-08
Rated 5 out of 5 by from Worked well in WB and IF in human MDAWorked well in WB and IF in human MDA-MB-231 cells and HEK293T cells. -SCBT Publication Review
Date published: 2014-02-22
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Rho G 抗体 (1F3 B3 E5) is rated 5.0 out of 5 by 8.
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