Date published: 2025-9-7

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Raptor 抗体 (10E10): sc-81537

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说明书
  • Raptor 抗体 10E10 是小鼠单克隆 IgG1Raptor抗体, 在43篇文献中引用,规格为50 µg/0.5 ml
  • 与human来源的部分Raptor对应的合成肽
  • 推荐用于 mouse, rat 和 human 来源的Raptor WB 和 IP检测; 也和以下物种反应,包括: canine
  • 关于如何获取Raptor (10E10): sc-81537免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Raptor Antibody (10E10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Raptor Antibody (10E10) 打包提供(请参阅下面的订购信息)。

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関連項目

Raptor抗体(10E10)是一种IgG1 κ小鼠单克隆Raptor抗体(也称为RPTOR抗体),可通过WB和IP检测小鼠、大鼠和人类的Raptor蛋白。Raptor抗体(10E10)为非偶联的抗Raptor抗体。FRAP的调节相关蛋白,也称为Raptor,是哺乳动物雷帕霉素激酶靶标(FRAP)的结合伙伴,对于FRAP在体内的信号传导至关重要。Raptor与FRAP的结合对于FRAP催化底物4E-BP1的磷酸化至关重要。raptor-FRAP复合物对营养敏感,对于细胞协调细胞生长和大小随环境变化而变化的机制很重要。在营养剥夺条件下,Raptor充当FRAP激酶活性的负调节剂,并且是FRAP途径中的重要组成部分。Raptor在骨骼肌中高表达,在脑、肾、肺和胎盘中表达程度较低。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Raptor 抗体 (10E10) 参考文献:

  1. 血清前白蛋白可预测血液透析和腹膜透析的存活率:10年的前瞻性观察。  |  Kim, DH., et al. 2002. Cell. 110: 163-75. PMID: 12150925
  2. 雷帕霉素靶蛋白(TOR)的结合伙伴 Raptor 是 TOR 作用的媒介。  |  Hara, K., et al. 2002. Cell. 110: 177-89. PMID: 12150926
  3. 雷帕霉素哺乳动物靶标(mTOR)的伴侣猛禽通过其 TOR 信号转导(TOS)基团与 mTOR 底物 p70 S6 激酶和 4E-BP1 结合。  |  Nojima, H., et al. 2003. J Biol Chem. 278: 15461-4. PMID: 12604610
  4. Raptor和mTOR:营养敏感复合体的亚单位。  |  Kim, DH. and Sabatini, DM. 2004. Curr Top Microbiol Immunol. 279: 259-70. PMID: 14560962
  5. 雷帕霉素靶标的结合伙伴 Raptor。  |  Yonezawa, K., et al. 2004. Biochem Biophys Res Commun. 313: 437-41. PMID: 14684181
  6. raptor与mTOR分离是雷帕霉素诱导抑制mTOR功能的一种机制。  |  Oshiro, N., et al. 2004. Genes Cells. 9: 359-66. PMID: 15066126
  7. S6 激酶 1 的结构决定了是猛禽-mTOR 还是雷克托-mTOR 使其疏水图案位点磷酸化。  |  Ali, SM. and Sabatini, DM. 2005. J Biol Chem. 280: 19445-8. PMID: 15809305
  8. 对营养敏感的猛禽-mTOR 途径和复合体的氧化还原调控。  |  Sarbassov, DD. and Sabatini, DM. 2005. J Biol Chem. 280: 39505-9. PMID: 16183647
  9. AMPK 磷酸化猛禽介导新陈代谢检查点。  |  Gwinn, DM., et al. 2008. Mol Cell. 30: 214-26. PMID: 18439900
  10. Raptor在淋巴细胞分化与功能中的作用。  |  Tang, J., et al. 2023. Front Immunol. 14: 1146628. PMID: 37283744
  11. 通过Syk/SHP2/Src/OTUB1轴介导的信号传导,抑制组织蛋白酶K可导致Raptor失稳和线粒体功能障碍。  |  Seo, SU., et al. 2023. Cell Death Dis. 14: 366. PMID: 37330581
  12. Raptor的O-GlcNAc化将葡萄糖信号传递给mTORC1。  |  Xu, C., et al. 2023. Mol Cell. 83: 3027-3040.e11. PMID: 37541260

订购信息

产品名称产品编号规格价格数量收藏夹

Raptor 抗体 (10E10)

sc-81537
50 µg/0.5 ml
$316.00

Raptor (10E10): m-IgG Fc BP-HRP 套装

sc-537248
50 µg Ab; 10 µg BP
$354.00

Raptor (10E10): m-IgGκ BP-HRP 套装

sc-534473
50 µg Ab; 40 µg BP
$354.00

Raptor (10E10): m-IgG1 BP-HRP 套装

sc-545104
50 µg Ab; 20 µg BP
$354.00

What is the recommended secondary to use in Western Blot with this antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, Mouse IgG kappa BP HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 4 out of 5 by from goodVery well signal was obtained from the western blotting.
Date published: 2022-03-04
Rated 5 out of 5 by from Excellent imageExcellent bands and a great antibody! In 3% milk / TBST
Date published: 2021-03-17
Rated 4 out of 5 by from Good antibodyThis antibody works well for WB but signal with normal ECL was too low. I used SuperSignal West Femto Chemiluminescent and the signal is OK. Dilution 1:1000 and overnight incubation. Dilution can be recycled.
Date published: 2019-11-21
Rated 4 out of 5 by from It works in human brain tissue.I used this antibody for western blot and it has a visible band (not so clean). I had to use a very high concentration (1:50) in 10 or 20 ug samples.
Date published: 2019-03-19
Rated 5 out of 5 by from It works well to detect expression of RaptorIt works well to detect expression of Rictor through WB and IF.
Date published: 2017-10-13
Rated 5 out of 5 by from test mouse testis through western blotSample: mouse adult testis. Result: very clear band for western blotting
Date published: 2017-08-15
Rated 5 out of 5 by from Great wbVery nice western blot results; strong signal, low background
Date published: 2017-01-03
Rated 5 out of 5 by from A good choice for Western blots!This antibody is a great choice for your Western blot analysis. It is reactive in all typical samples and will provide clean and clear bands for you. It has over ten publications to support it.
Date published: 2016-12-29
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Raptor抗体 (10E10) is rated 4.8 out of 5 by 13.
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