Rap1GAP Antibody (G-1): sc-514543
- Rap1GAP Antibody (G-1) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 613-636 near the C-terminus of Rap1GAP of human origin
- recommended for detection of Rap1GAP of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG3 BP-HRP is the preferred secondary detection reagent for Rap1GAP Antibody (G-1) for WB applications. This reagent is now offered in a bundle with Rap1GAP Antibody (G-1) (see ordering information below).
QUICK LINKS
SEE ALSO...
Rap1GAP Antibody (G-1) is a mouse monoclonal IgG3 antibody that detects Rap1GAP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rap1GAP plays a crucial role in cellular signaling by specifically stimulating the GTP hydrolytic activity of the monomeric G protein Rap1, which is essential for regulating various cellular processes, including cell proliferation and differentiation. Rap1GAP is predominantly located in the brain, kidney, and pancreas, where Rap1GAP acts as a signal integrator, coordinating Gz signaling and Rap1 signaling pathways. Notably, a novel isoform of Rap1GAP, known as Rap1GAPII, has been identified to bind specifically to Gαz, a member of the Gi family of trimeric G proteins. This interaction blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the α subunit, thereby modulating downstream effects on adenylyl cyclase activity. Furthermore, stimulation of the Gi-coupled M2 muscarinic receptor leads to Rap1GAPII translocation from cytosol to membrane, resulting in a decrease in GTP-bound Rap1 and subsequent activation of the ERK/MAPK signaling pathway. anti-Rap1GAP antibody (G-1) is an invaluable tool for researchers studying these complex signaling mechanisms and their implications in various physiological and pathological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rap1GAP Antibody (G-1) References:
- Functional interaction between Galpha(z) and Rap1GAP suggests a novel form of cellular cross-talk. | Meng, J., et al. 1999. J Biol Chem. 274: 36663-9. PMID: 10593970
- Purification, crystallization and preliminary structural characterization of human Rap1GAP. | Daumke, O., et al. 2004. Acta Crystallogr D Biol Crystallogr. 60: 752-4. PMID: 15039575
- The GTPase-activating protein Rap1GAP uses a catalytic asparagine. | Daumke, O., et al. 2004. Nature. 429: 197-201. PMID: 15141215
- Protein RAP1GAP in human myelodysplastic syndrome detected by flow cytometry and its clinical relevance. | Ika, SA., et al. 2009. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 17: 612-7. PMID: 19549374
- The GTPase-activating protein Rap1GAP: a new player to modulate Ret signaling. | Paratcha, G. and Ledda, F. 2011. Cell Res. 21: 217-9. PMID: 20956997
- Histone deacetylase inhibitors upregulate Rap1GAP and inhibit Rap activity in thyroid tumor cells. | Dong, X., et al. 2011. Endocr Relat Cancer. 18: 301-10. PMID: 21367844
- Notoginsenoside R2 induces colonic microvascular injuries via regulating the Rap1GAP/PI3K/Akt signaling pathway. | Tao, P., et al. 2021. Ann Transl Med. 9: 1743. PMID: 35071437
- ADAM10 facilitates rapid neural stem cell cycling and proper positioning within the subventricular zone niche via JAMC/RAP1Gap signaling. | McMillan, N., et al. 2022. Neural Regen Res. 17: 2472-2483. PMID: 35535899
- Identification of tuberin, the tuberous sclerosis-2 product. Tuberin possesses specific Rap1GAP activity. | Wienecke, R., et al. 1995. J Biol Chem. 270: 16409-14. PMID: 7608212
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rap1GAP Antibody (G-1) | sc-514543 | 200 µg/ml | $322.00 | |||
Rap1GAP Antibody (G-1): m-IgG3 BP-HRP Bundle | sc-550520 | 200 µg Ab; 40 µg BP | $361.00 | |||
Rap1GAP (G-1) Neutralizing Peptide | sc-514543 P | 100 µg/0.5 ml | $69.00 |