Date published: 2025-9-7

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Rap1GAP 抗体 (D-9): sc-166586

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说明书
  • Rap1GAP 抗体 D-9 是小鼠单克隆 IgG2a κ,Rap1GAP抗体, 在4篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Rap1GAP的N-terminus附近的氨基酸23-40之间
  • Rap1GAP 抗体 (D-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Rap1GAP; 也和以下物种反应,包括: 和 canine and bovine
  • 抗Rap1GAP抗体(D-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rap1GAP (D-9): sc-166586免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 Rap1GAP 抗体 (D-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Rap1GAP 抗体 (D-9) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Rap1GAP抗体(D-9)是一种IgG2a κ小鼠单克隆Rap1GAP抗体(也称为Rap1GAP抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的Rap1GAP蛋白。Rap1GAP抗体(D-9)既可作为非偶联的抗Rap1GAP抗体形式提供,也可作为多种偶联形式的抗Rap1GAP抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Rap1 GTPase激活蛋白(Rap1GAP)特异性地刺激单体G蛋白Rap1的GTP水解活性。三聚体G蛋白Gi家族的成员Gαz与Rap1GAP之间的物理相互作用,阻断了G蛋白信号调节剂刺激α亚基GTP水解的能力,并减弱了活化的Gαz抑制腺苷酸环化酶的能力。Rap1GAP在脑、肾和胰腺中表达,并可能作为信号整合器来协调和/或整合细胞中的Gz信号和Rap1信号。一种新型Rap1 GTPase激活蛋白异构体,被命名为Rap1GAPII,它特异性地与Gαz结合。Gi偶联的M2毒蕈碱受体的刺激使Rap1GAPII从细胞质转移到细胞膜,并减少了与GTP结合的Rap1的数量,从而导致ERK/MAPK的激活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rap1GAP 抗体 (D-9) 参考文献:

  1. Galpha(o) 与 Rap1 GTPase-activating 蛋白的直接相互作用调节 Rap 的活性。  |  Jordan, JD., et al. 1999. J Biol Chem. 274: 21507-10. PMID: 10419452
  2. 与 G alpha(i)相关的一种 rap1GAP 异构体激活 ERK/MAPK 通路。  |  Mochizuki, N., et al. 1999. Nature. 400: 891-4. PMID: 10476970
  3. Galpha(z)与 Rap1GAP 之间的功能性相互作用表明,这是一种新型的细胞交叉对话形式。  |  Meng, J., et al. 1999. J Biol Chem. 274: 36663-9. PMID: 10593970
  4. Rap1GAP在不影响细胞间粘附的情况下影响细胞-基质粘附。  |  Vuchak, LA., et al. 2011. Cell Adh Migr. 5: 323-31. PMID: 21785277
  5. Rap1GAP 可抑制子宫内膜癌的肿瘤进展。  |  Tamate, M., et al. 2017. Biochem Biophys Res Commun. 485: 476-483. PMID: 28196746
  6. Rap1GAP表达降低作为原发性胃癌患者的预后生物标志物。  |  Zhao, J., et al. 2018. Cancer Biomark. 22: 375-384. PMID: 29758923
  7. Rap1GAP的甲基化作用水平及其在骨髓增生异常综合征中的临床意义。  |  Ding, WJ., et al. 2018. Oncol Lett. 16: 7287-7294. PMID: 30546468
  8. Rap1GAP通过抑制自噬和增加氧化应激来介导血管紧张素II诱导的心肌细胞肥大。  |  Gao, Y., et al. 2021. Oxid Med Cell Longev. 2021: 7848027. PMID: 33936386
  9. Rap1GAP通过调节AMPK/SIRT1/NF-κB信号通路加重心肌梗塞。  |  Shan, T., et al. 2024. Cell Signal. 117: 111080. PMID: 38320624
  10. 细胞周期中 Rap1GAP 的磷酸化。  |  Janoueix-Lerosey, I., et al. 1994. Biochem Biophys Res Commun. 202: 967-75. PMID: 8048970
  11. 丝裂原诱导型 SIPA1 在小鼠中被映射到 19 号染色体的保守同源组上,在人类中被映射到与 BCL1 同源的 11q13.3 号染色体上。  |  Wada, Y., et al. 1997. Genomics. 39: 66-73. PMID: 9027487
  12. 选择性表达于淋巴组织的人类 SPA-1 基因产物是 Rap1 和 Rap2 的特异性 GTPase 激活蛋白。rap1GAP基因产物的分离表达谱。  |  Kurachi, H., et al. 1997. J Biol Chem. 272: 28081-8. PMID: 9346962

订购信息

产品名称产品编号规格价格数量收藏夹

Rap1GAP 抗体 (D-9)

sc-166586
200 µg/ml
$316.00

Rap1GAP (D-9): m-IgG Fc BP-HRP 套装

sc-528999
200 µg Ab; 10 µg BP
$354.00

Rap1GAP (D-9): m-IgGκ BP-HRP 套装

sc-521668
200 µg Ab, 40 µg BP
$354.00

Rap1GAP (D-9): m-IgG2a BP-HRP 套装

sc-547232
200 µg Ab; 10 µg BP
$354.00

Rap1GAP 抗体 (D-9) AC

sc-166586 AC
500 µg/ml, 25% agarose
$416.00

Rap1GAP 抗体 (D-9) HRP

sc-166586 HRP
200 µg/ml
$316.00

Rap1GAP 抗体 (D-9) FITC

sc-166586 FITC
200 µg/ml
$330.00

Rap1GAP 抗体 (D-9) PE

sc-166586 PE
200 µg/ml
$343.00

Rap1GAP 抗体 (D-9) Alexa Fluor® 488

sc-166586 AF488
200 µg/ml
$357.00

Rap1GAP 抗体 (D-9) Alexa Fluor® 546

sc-166586 AF546
200 µg/ml
$357.00

Rap1GAP 抗体 (D-9) Alexa Fluor® 594

sc-166586 AF594
200 µg/ml
$357.00

Rap1GAP 抗体 (D-9) Alexa Fluor® 647

sc-166586 AF647
200 µg/ml
$357.00

Rap1GAP 抗体 (D-9) Alexa Fluor® 680

sc-166586 AF680
200 µg/ml
$357.00

Rap1GAP 抗体 (D-9) Alexa Fluor® 790

sc-166586 AF790
200 µg/ml
$357.00

Rap1GAP (D-9) 中和勝肽

sc-166586 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-166586 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Worked wellDetected human lung cancer tissue extracts (40ug),very happy result with strong band (1:800 dilution) at 90kda,we suggest 4℃ overnight incubated and use the secondary sc-516102 from Sanata Cruz.
Date published: 2017-09-10
Rated 5 out of 5 by from Exellent antibodyI got this antibody sample for free. It works excellently.
Date published: 2017-08-24
Rated 5 out of 5 by from western blotIt worked with succes for western blot analysis Rap1GAP ,the antibody canbe diluted 1:400 or more lower up to 1:1000.Suggest use Hela cell as positive control.
Date published: 2017-08-12
Rated 5 out of 5 by from strong signalThis antibody is strong binding the target Rap1GAP,we suggest use low conceration at 1:1000,this conceration is no background, but signal is still strong for us.
Date published: 2017-08-12
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-03-03
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Rap1GAP 抗体 (D-9) is rated 5.0 out of 5 by 6.
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