Date published: 2025-9-6
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Rap 1 Antibody (E-6) - Western Blotting - Image 354078
Rap 1 抗体 (E-6): sc-398755. A-431 (A), C6 (B), MOLT-4 (C), HUV-EC-C (D) 和 RAW 264. 7 (E) 全细胞裂解液及小鼠脑组织提取物 (F) 中 Rap 1 表达的 Western 印迹分析.
Rap 1 抗体 (E-6): sc-398755. A-431 (A), C6 (B), MOLT-4 (C), HUV-EC-C (D) 和 RAW 264. 7 (E) 全细胞裂解液及小鼠脑组织提取物 (F) 中 Rap 1 表达的 Western 印迹分析.

Rap 1 抗体 (E-6): sc-398755

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说明书
  • Rap 1 抗体 E-6 是小鼠单克隆 IgG1 κ，Rap 1抗体, 在15篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Rap 1A的C-terminus附近的氨基酸140-174之间
  • Rap 1 抗体 (E-6) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Rap 1A and Rap 1B
  • 抗Rap 1抗体（E-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rap 1 (E-6): sc-398755的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Rap 1 Antibody (E-6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Rap 1 Antibody (E-6) 打包提供（请参阅下面的订购信息）。

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描述
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研究信息

関連項目

额外RAS-related Rap抗体，包括Rap 1, Rap 1A和Rap 2

Rap 1抗体（E-6）是一种IgG1 κ小鼠单克隆Rap 1抗体（也称为RAP1抗体或Rap1A/Rap1B抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Rap 1蛋白。Rap 1抗体（E-6）既有非偶联型抗Rap 1抗体形式，也有多种偶联型抗Rap 1抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ras癌基因编码GTP结合蛋白，能够在培养中转化永生细胞。两个与Ras相关的人类基因，分别命名为RAP1A和RAP1B，编码95%的同源蛋白，这些蛋白与Ras蛋白共享相似的C末端Cys-Ali-Ali-Xaa序列，并在哺乳动物组织中普遍表达。Rap 1蛋白中假定的"效应器"结构域在细胞转化以及与假定的效应器蛋白GAP相互作用中保持完整，这两种Rap 1蛋白都保守。据推测，p21Rap 1通过与Ras GAP结合来干扰Ras效应器功能。事实上，已知p21Rap 1在体外以GTP依赖的方式与Ras GAP结合，而不影响p21Rap 1的GTP酶活性。已经鉴定出特异于p21Rap 1的GAP蛋白，并且已经分离出相应的cDNA。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rap 1 抗体 (E-6) 参考文献:

  1. Ras 拮抗物 Rap1/Krev-1 蛋白与高尔基复合体的关联。  |  Béranger, F., et al. 1991. Proc Natl Acad Sci U S A. 88: 1606-10. PMID: 1900364
  2. 克雷夫-1 蛋白 p21rap1 特异性 GTPase 激活蛋白的分子克隆。  |  Rubinfeld, B., et al. 1991. Cell. 65: 1033-42. PMID: 1904317
  3. smg-21/rap1/Krev-1 蛋白的组织和亚细胞分布与 c-ras p21s 蛋白部分不同。  |  Kim, S., et al. 1990. Mol Cell Biol. 10: 2645-52. PMID: 2111441
  4. 与 ras p21s 具有相同推定效应结构域的 smg p21 抑制 c-Ha-ras p21 的 ras p21 GTPase-activating protein 激化 GTPase 活性。  |  Hata, Y., et al. 1990. J Biol Chem. 265: 7104-7. PMID: 2158984
  5. Krev-1 基因产物抑制 GTPase 活化蛋白对 Ras-p21 GTPase 的刺激。  |  Frech, M., et al. 1990. Science. 249: 169-71. PMID: 2164710
  6. 人类肿瘤中 ras 相关 rap 基因的表达。  |  Culine, S., et al. 1989. Int J Cancer. 44: 990-4. PMID: 2514150
  7. 与果蝇基因 Dras3 同源的人类 cDNA rap1 和 rap2 在 '效应区 '编码与 ras 密切相关的蛋白质。  |  Pizon, V., et al. 1988. Oncogene. 3: 201-4. PMID: 3045729
  8. 编码 ras 相关蛋白（rap1B）的人类 cDNA 的核苷酸序列。  |  Pizon, V., et al. 1988. Nucleic Acids Res. 16: 7719. PMID: 3137530
  9. Epac-1/Rap-1信号通路协调替卡格雷对肾缺血/再灌注模型的肾治疗作用。  |  El-Mokadem, BM., et al. 2021. Biomed Pharmacother. 139: 111488. PMID: 33957564
  10. 中心体扩增通过细胞间接触破坏和Rap-1激活促进细胞侵袭。  |  Prakash, A., et al. 2023. J Cell Sci. 136: PMID: 37772773
  11. Syndapin和GTPase RAP-1通过RHO-1和非肌动蛋白II控制内吞循环。  |  Rodriguez-Polanco, WR., et al. 2023. Curr Biol. 33: 4844-4856.e5. PMID: 37832552
  12. 对巴贝虫RAP-1蛋白的结构定义确定了整个顶复动物的新蛋白超家族。  |  Hötzel, I. and Suarez, CE. 2023. Sci Rep. 13: 22330. PMID: 38102310

订购信息

产品名称产品编号规格价格数量收藏夹

Rap 1 抗体 (E-6)

sc-398755
200 µg/ml
$316.00

Rap 1 (E-6): m-IgG Fc BP-HRP 套装

sc-530917
200 µg Ab; 10 µg BP
$354.00

Rap 1 (E-6): m-IgGκ BP-HRP 套装

sc-524471
200 µg Ab, 40 µg BP
$354.00

Rap 1 (E-6): m-IgG1 BP-HRP 套装

sc-544317
200 µg Ab; 20 µg BP
$354.00

Rap 1 抗体 (E-6) AC

sc-398755 AC
500 µg/ml, 25% agarose
$416.00

Rap 1 抗体 (E-6) HRP

sc-398755 HRP
200 µg/ml
$316.00

Rap 1 抗体 (E-6) FITC

sc-398755 FITC
200 µg/ml
$330.00

Rap 1 抗体 (E-6) PE

sc-398755 PE
200 µg/ml
$343.00

Rap 1 抗体 (E-6) Alexa Fluor® 488

sc-398755 AF488
200 µg/ml
$357.00

Rap 1 抗体 (E-6) Alexa Fluor® 546

sc-398755 AF546
200 µg/ml
$357.00

Rap 1 抗体 (E-6) Alexa Fluor® 594

sc-398755 AF594
200 µg/ml
$357.00

Rap 1 抗体 (E-6) Alexa Fluor® 647

sc-398755 AF647
200 µg/ml
$357.00

Rap 1 抗体 (E-6) Alexa Fluor® 680

sc-398755 AF680
200 µg/ml
$357.00

Rap 1 抗体 (E-6) Alexa Fluor® 790

sc-398755 AF790
200 µg/ml
$357.00

Rap 1 (E-6) 中和勝肽

sc-398755 P
100 µg/0.5 ml
$68.00

Does this antibody detect unprenylated and total Rap1A?

Asked by: Anonymous
Thank you for your question. Rap 1 Antibody (E-6): sc-398755 detects both unprenylated and prenylated Rap1A. It was raised against amino acids 140-174 of Rap1A.
Answered by: Tech Support Europe
Date published: 2020-09-22

Is the sc-398755 antibody a function-blocking antibody for Rap1, as was SC-65?

Asked by: dcm12
This antibody has not been tested for functional or blocking activity.
Answered by: Technical Support
Date published: 2018-12-14

Hi, could you give the conditions for a WB with this antibody please ? (i.e PVDF or nitrocellulose membrane, milk or BSA) Also the citation listed in the datasheet does not mention this antibody. Cheers

Asked by: Julie548
Thank you for your question. The recommended protocol for Western blotting with this mouse monoclonal antibody, Rap 1 (E-6): sc-398755, is provided below. We recommend using PVDF (sc-3723) transfer membrane. For blocking and antibody diluent we recommend using 5% nonfat milk + TBST (TBS Blotto A: sc-2333). https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting There is one publication citing use of this antibody at this time, please see PMID: # 26785133. The "References" listed on the product data sheet are literature references used to write the general background information about the Rap 1 protein. These are not product citations for the antibody.
Answered by: Technical Support
Date published: 2017-04-28

What application is the blocking peptide sc-398755 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Western blotIt is specific and detect Rap 1 with or without an isoprenyl group.
Date published: 2019-08-14
Rated 5 out of 5 by from Great antibody for WBI used this antibody for WB to detect Rap 1 expression in mouse brain from a glioblastoma-initiating cell xenograft model. I am happy I got very good band.
Date published: 2018-01-02
Rated 5 out of 5 by from Specific bandI used this antibody on HUVEC (human umbilical vein endothelial cells) lysates and it worked well (overnight incubation with primary antibody, then using LICOR secondary antibody) - single band seen at expected MW.
Date published: 2017-10-12
Rated 5 out of 5 by from specific/sharp bands detectedThis antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with no background.
Date published: 2017-09-14
Rated 5 out of 5 by from Works greatBought this antibody (RAP1) to measure protein-protein interactions (amongst other things) in 293T/HeLa lysates. Worked great! WB 1:1000 and IF/PLA 1:200. Works alongside irradiated samples/samples with small molecules really well. Looking to expand this to using alongside HTS platforms!
Date published: 2017-08-20
Rated 5 out of 5 by from Works for WB/IF/PLABought this antibody (RAP1) to measure protein-protein interactions (amongst other things) in 293T/HeLa lysates. Worked great! WB 1:1500 and IF/PLA 1:100.
Date published: 2017-08-15
Rated 4 out of 5 by from WELL BY WBIT WORKED WELL BY WB IN LAB, WILL PURCHASE IT IN THE FUTURE
Date published: 2017-02-20
Rated 5 out of 5 by from Modified and unmodified Rap1 detectedWe were able to detect unmodified and modified Rap1 using this antibody
Date published: 2017-02-15
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Rap 1 抗体 (E-6) is rated 4.9 out of 5 by 10.
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