Date published: 2025-9-6
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Rac 1/2/3 Antibody (G-2) - Western Blotting - Image 375595
Rac 1/2/3 抗体 (G-2): sc-514583. PC-3 (A), A-431 (B), NIH/3T3 (C) 和 F9 (D) 全细胞裂解液中 Rac 1/2/3 表达的 Western 印迹分析.
Rac 1/2/3 抗体 (G-2): sc-514583. PC-3 (A), A-431 (B), NIH/3T3 (C) 和 F9 (D) 全细胞裂解液中 Rac 1/2/3 表达的 Western 印迹分析.

Rac 1/2/3 抗体 (G-2): sc-514583

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寫評論提問

说明书
  • Rac 1/2/3 抗体 G-2 是小鼠单克隆 IgG2b κ，Rac 1/2/3抗体, 在25篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Rac 1的C-terminus附近的氨基酸163-183之间
  • Rac 1/2/3 抗体 (G-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Rac 1, Rac 2 and Rac 3
  • 抗Rac 1/2/3抗体（G-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rac 1/2/3 (G-2): sc-514583的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Rac 1/2/3 抗体 (G-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Rac 1/2/3 抗体 (G-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Rac 抗体包括 Rac 1 和 Rac 2

Rac 1/2/3抗体（G-2）是一种鼠单克隆IgG2b kappa轻链抗体，用于检测小鼠、大鼠和人类来源的Rac1、Rac2和Rac3蛋白，可用于多种应用，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）。Rac 1/2/3 (G-2) 单克隆抗体靶向人类 Rac1 蛋白 C 末端附近 163-183 氨基酸之间的精确表位，确保在不同实验需求下都能实现稳定可靠的结合。Rac 蛋白是 Ras 超家族小 GTP 酶的组成部分，在调节细胞生长、囊泡运输和细胞骨架组织等多种基本细胞过程方面发挥着关键作用。具体来说，Rac1在多种细胞类型中普遍表达，通过调节肌动蛋白细胞骨架的动态变化，在控制细胞形态、迁移和增殖方面发挥着关键作用。Rac2主要在髓细胞中表达，对于调节中性粒细胞中的NADPH氧化酶复合物至关重要，从而显著促进免疫反应和活性氧的产生。Rac 1/2/3 (G-2) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 结合物，为不同的研究应用提供了多功能性。通过靶向这些关键调节蛋白，抗Rac 1/2/3 (G-2)抗体有助于研究细胞动力学、免疫功能和相关病理条件所涉及的关键信号通路，对于旨在阐明健康和疾病分子机制的研究人员来说，这种工具是必不可少的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rac 1/2/3 抗体 (G-2) 参考文献:

  1. GTP 结合蛋白 Rac 2 对吞噬细胞氧自由基生成的调节。  |  Knaus, UG., et al. 1991. Science. 254: 1512-5. PMID: 1660188
  2. Bcr 编码 p21rac 的 GTPase 激活蛋白。  |  Diekmann, D., et al. 1991. Nature. 351: 400-2. PMID: 1903516
  3. 小 GTP 结合蛋白的细胞功能。  |  Hall, A. 1990. Science. 249: 635-40. PMID: 2116664
  4. 神经纤维瘤病 1 型基因产物的催化结构域可刺激 ras GTPase 并补充 S. cerevisiae 的 ira 突变体。  |  Xu, GF., et al. 1990. Cell. 63: 835-41. PMID: 2121369
  5. 神经纤维瘤病 1 型基因产物的 GAP 相关结构域与 ras p21 相互作用。  |  Martin, GA., et al. 1990. Cell. 63: 843-9. PMID: 2121370
  6. NF1 基因座编码一种在功能上与哺乳动物 GAP 和酵母 IRA 蛋白相关的蛋白质。  |  Ballester, R., et al. 1990. Cell. 63: 851-9. PMID: 2121371
  7. rac，一种与 ras 相关的新型蛋白质家族，是肉毒毒素的底物。  |  Didsbury, J., et al. 1989. J Biol Chem. 264: 16378-82. PMID: 2674130
  8. 一种细胞质蛋白可刺激正常的 N-ras p21 GTPase，但不会影响致癌突变体。  |  Trahey, M. and McCormick, F. 1987. Science. 238: 542-5. PMID: 2821624
  9. 牛和酵母 ADP-ribosylation 因子的序列以及与其他 GTP 结合蛋白的比较。  |  Sewell, JL. and Kahn, RA. 1988. Proc Natl Acad Sci U S A. 85: 4620-4. PMID: 3133654

订购信息

产品名称产品编号规格价格数量收藏夹

Rac 1/2/3 抗体 (G-2)

sc-514583
200 µg/ml
$316.00

Rac 1/2/3 (G-2): m-IgG Fc BP-HRP 套装

sc-531169
200 µg Ab; 10 µg BP
$354.00

Rac 1/2/3 (G-2): m-IgGκ BP-HRP 套装

sc-524781
200 µg Ab, 40 µg BP
$354.00

Rac 1/2/3 (G-2): m-IgG2b BP-HRP 套装

sc-550265
200 µg Ab; 10 µg BP
$354.00

Rac 1/2/3 抗体 (G-2) AC

sc-514583 AC
500 µg/ml, 25% agarose
$416.00

Rac 1/2/3 抗体 (G-2) HRP

sc-514583 HRP
200 µg/ml
$316.00

Rac 1/2/3 抗体 (G-2) FITC

sc-514583 FITC
200 µg/ml
$330.00

Rac 1/2/3 抗体 (G-2) PE

sc-514583 PE
200 µg/ml
$343.00

Rac 1/2/3 抗体 (G-2) Alexa Fluor® 488

sc-514583 AF488
200 µg/ml
$357.00

Rac 1/2/3 抗体 (G-2) Alexa Fluor® 546

sc-514583 AF546
200 µg/ml
$357.00

Rac 1/2/3 抗体 (G-2) Alexa Fluor® 594

sc-514583 AF594
200 µg/ml
$357.00

Rac 1/2/3 抗体 (G-2) Alexa Fluor® 647

sc-514583 AF647
200 µg/ml
$357.00

Rac 1/2/3 抗体 (G-2) Alexa Fluor® 680

sc-514583 AF680
200 µg/ml
$357.00

Rac 1/2/3 抗体 (G-2) Alexa Fluor® 790

sc-514583 AF790
200 µg/ml
$357.00

Rac 1/2/3 (G-2) 中和勝肽

sc-514583 P
100 µg/0.5 ml
$68.00

I want to detect Rac1, if I use this pan Rac antibody, could I interpret my result?

Asked by: Annie_dr
Thank you for your question. Since this pan Rac antibody will detect Rac 1, Rac 2 and Rac 3, there is no way to distinguish which protein is present of those three in a positive signal. It will react with Rac 1 if present, but unless the sample is known to be negative for Rac 2 and Rac 3 you will not be able to say definitely that the signal is Rac 1 and not Rac 2 or Rac 3. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-04-24

What application is the blocking peptide sc-514583 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from works wellI tried the sample last month and it gave me good results!
Date published: 2018-03-19
Rated 2 out of 5 by from Non-specific bindingUsed a 1:300 dilution for WB with Sum159, Caco-2, and 293T cell lysates. Many non-specific bands present, some stronger than rac.
Date published: 2017-11-30
Rated 5 out of 5 by from Very sensitive antibodyI blocked my Western blot in 5% BSA and diluted the antibody 1:500 overnight at 4 degrees C. The band was very intense, and there were a lot of bands above the 23k band albeit it weaker in intensity. I could've easily have used a 1:1500 dilution or more. The antibody worked very well.
Date published: 2017-11-22
Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 4 out of 5 by from Good for IF and IHCThis pan Rac antibody shows strong specific signal in IF and IHC stainings in primary rat motor neuron.
Date published: 2017-11-06
Rated 5 out of 5 by from stains very brightly in cellsDilution 1:500, it without background and good work. It has high sensitivity and good stability.
Date published: 2017-10-27
Rated 5 out of 5 by from Work perfectlyUse at 1:400 on human primary macrophages and wok perfectly without any other bands or background.
Date published: 2017-10-17
Rated 5 out of 5 by from pan Rac antibodyI used this antibody on HUVEC (human umbilical vein endothelial cells) lysates and I saw a single band at expected MW (overnight incubation with primary antibody, then using LICOR secondary antibody).
Date published: 2017-10-12
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Rac 1/2/3 抗体 (G-2) is rated 4.3 out of 5 by 21.
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