Date published: 2025-9-6
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PP2B-Aα Antibody (D-9) - Western Blotting - Image 373532
PP2B-Aα 抗体 (D-9): sc-17808. 小鼠大脑 (A), 人类大脑皮层 (B) 和大鼠大脑 (C) 组织提取物中 PP2B-Aα 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
PP2B-Aα 抗体 (D-9): sc-17808. 小鼠大脑 (A), 人类大脑皮层 (B) 和大鼠大脑 (C) 组织提取物中 PP2B-Aα 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

PP2B-Aα 抗体 (D-9): sc-17808

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说明书
  • PP2B-A alpha 抗体 D-9 是小鼠单克隆 IgG2b κ，PP2B-Aα抗体, 在16篇文献中引用，规格为200 µg/ml
  • 免疫human物种的protein phosphatase 2B catalytic Aα subunit (PP2B-Aα)的氨基酸312-521
  • PP2B-A alpha 抗体 (D-9) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 PP2B-Aα; 也和以下物种反应，包括： 和 equine and canine
  • 抗PP2B-A alpha抗体（D-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PP2B-Aα (D-9): sc-17808的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是PP2B-Aα Antibody (D-9) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PP2B-Aα Antibody (D-9) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外PP2抗体，包括PP2A-A α, PP2A-A alpha / β, PP2A-B55, PP2A-B55-α, PP2A-B55-γ, PP2A-B56-α, PP2A-B56-δ, PP2A-B56-γ, PP2A-C, PP2A-C alpha / β, PP2B-A α, PP2B-A β, PP2B-A γ, PP2B-B1, PP2B-B2和PP2C α

PP2B-Aα抗体（D-9）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的PP2B-Aα蛋白。抗PP2B-A alpha抗体（D-9）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。PP2B-Aα蛋白，也称为钙调神经磷酸酶Aα，通过去磷酸化目标蛋白上的丝氨酸和苏氨酸残基，在钙依赖性信号传导通路中发挥关键作用，这对于调节T细胞活化、肌肉收缩和神经信号传导等多种细胞过程至关重要。PP2B-A alpha 主要位于细胞质中，由钙结合的钙调蛋白激活，这凸显了 PP2B-A alpha 在将钙信号转化为细胞反应中的重要性。PP2B-A alpha 与钙调蛋白的相互作用对于酶活性至关重要，这种相互作用失调会导致各种病理生理状况，包括自身免疫性疾病和心脏功能障碍。在研究中使用PP2B-Aα（D-9）单克隆抗体，可以深入了解钙信号的调节机制及其对健康和疾病的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PP2B-Aα 抗体 (D-9) 参考文献:

  1. 小鼠钙调蛋白依赖性蛋白磷酸酶调节亚基（钙调蛋白 B）两种异构体的结构和表达。  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
  2. 激活钙神经蛋白-Aα可增强肌毒性损伤后再生肌肉的结构和功能。  |  Stupka, N., et al. 2007. Am J Physiol Regul Integr Comp Physiol. 293: R686-94. PMID: 17475677
  3. 钙调素 A alpha 基因缺失的细胞和分子后果  |  Sun, L., et al. 2007. Ann N Y Acad Sci. 1116: 216-26. PMID: 17872390
  4. 钙神经蛋白 A-α 抑制驱动核因子-κB 介导的 NADPH 氧化酶-2 上调。  |  Cheriyan, AM., et al. 2021. Am J Physiol Renal Physiol. 320: F789-F798. PMID: 33615888
  5. 丝氨酸/苏氨酸蛋白磷酸酶。  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
  6. EL4 淋巴瘤细胞表达的突变钙调素 A alpha 基因的特征。  |  Fruman, DA., et al. 1995. Mol Cell Biol. 15: 3857-63. PMID: 7791792
  7. 新型蛋白磷酸酶对丝氨酸和苏氨酸残基去磷酸化的重要作用  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
  8. 平滑肌肌球蛋白结合磷酸酶的调节亚基。  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
  9. 蛋白磷酸酶 2A 72 kDa 调节亚基的结构和表达。替代剪接产生不同大小形式的证据。  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
  10. 编码人钙调素依赖性蛋白磷酸酶催化亚基（钙调素 A alpha）的全长 cDNA 的分子克隆。  |  Muramatsu, T. and Kincaid, RL. 1993. Biochim Biophys Acta. 1178: 117-20. PMID: 8392375
  11. 蛋白丝氨酸/苏氨酸磷酸酶：结构, 调节和在细胞生长中的功能。  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923
  12. 从 Ser/Thr 磷酸酶钙调磷酸酶和蛋白磷酸酶 1 的结构衍生物中获得的启示。  |  Lohse, DL., et al. 1995. Structure. 3: 987-90. PMID: 8590008

订购信息

产品名称产品编号规格价格数量收藏夹

PP2B-Aα 抗体 (D-9)

sc-17808
200 µg/ml
$316.00

PP2B-Aα (D-9): m-IgG Fc BP-HRP 套装

sc-528321
200 µg Ab; 10 µg BP
$354.00

PP2B-Aα (D-9): m-IgGκ BP-HRP 套装

sc-520666
200 µg Ab, 40 µg BP
$354.00

PP2B-Aα (D-9): m-IgG2b BP-HRP 套装

sc-550028
200 µg Ab; 10 µg BP
$354.00

PP2B-Aα 抗体 (D-9) AC

sc-17808 AC
500 µg/ml, 25% agarose
$416.00

PP2B-Aα 抗体 (D-9) HRP

sc-17808 HRP
200 µg/ml
$316.00

PP2B-Aα 抗体 (D-9) FITC

sc-17808 FITC
200 µg/ml
$330.00

PP2B-Aα 抗体 (D-9) PE

sc-17808 PE
200 µg/ml
$343.00

PP2B-Aα 抗体 (D-9) Alexa Fluor® 488

sc-17808 AF488
200 µg/ml
$357.00

PP2B-Aα 抗体 (D-9) Alexa Fluor® 546

sc-17808 AF546
200 µg/ml
$357.00

PP2B-Aα 抗体 (D-9) Alexa Fluor® 594

sc-17808 AF594
200 µg/ml
$357.00

PP2B-Aα 抗体 (D-9) Alexa Fluor® 647

sc-17808 AF647
200 µg/ml
$357.00

PP2B-Aα 抗体 (D-9) Alexa Fluor® 680

sc-17808 AF680
200 µg/ml
$357.00

PP2B-Aα 抗体 (D-9) Alexa Fluor® 790

sc-17808 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with PP2B-Aα (D-9): sc-17808 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from マウス脳凍結切片の免疫染色にワークマウス脳凍結切片を抗原不活化（クエン酸バッファー:MW処理5min)して200倍希釈でよく染まります。
Date published: 2020-10-02
Rated 4 out of 5 by from It works wellThe sample of this product works well. I got a thin bind of my protein sample from osteoclasts.
Date published: 2018-03-27
Rated 5 out of 5 by from Works for IPWorks for IP. Had previously tried calcineurin antibody from CST which didn't work.
Date published: 2017-12-26
Rated 4 out of 5 by from Good clean bands, sadly several productsSadly, there were more than one products turning up for western blots (1:1000) using this, but otherwise, nice clean bands where I needed them.
Date published: 2017-11-07
Rated 5 out of 5 by from Calcineurin antibodyMouse anti-CNalpha antibody (sc-17808) works great on immunoblotting at 1:1000 in 2.5 % milk powder. It can be reused and recognizes a 60 kDa band in all blood cancer cells tested in my lab including J77 cells.
Date published: 2017-08-24
Rated 3 out of 5 by from Work for WBUse siPP2B MEF cell sample, Ab diluted to 1:1000, got the single and clear band at the right site.
Date published: 2017-08-17
Rated 5 out of 5 by from Excellent antibody for Western blotting.This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It very strongly detects an appropriately sized ~59 kDa target protein in immunoblots.
Date published: 2016-10-13
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic staining in methanol-fixed EOC 20 cells. -SCBT QC
Date published: 2015-05-04
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PP2B-Aα 抗体 (D-9) is rated 4.6 out of 5 by 12.
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