PKC lambda/iota Antibody (H-12): sc-17837

PKC λ/ι Antibody (H-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects PKC lambda/iota of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PKC λ/ι (H-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PKC lambda/iota plays a crucial role in regulating cellular functions such as cell growth, differentiation, and signaling pathways, which are vital for maintaining cellular homeostasis and responding to external stimuli. Located primarily in the cytoplasm and translocating to the membrane upon activation, PKC lambda/iota is involved in modulating various signaling cascades, including those related to cell survival and apoptosis. A unique structure, characterized by a C1 domain that binds diacylglycerol and phorbol esters, allows PKC lambda/iota to respond to lipid second messengers, making PKC lambda/iota an essential player in the phosphoinositide signaling pathway. Understanding PKC lambda/iota function and localization is important for elucidating PKC lambda/iota′s role in diseases such as cancer and neurodegenerative disorders, where dysregulation of PKC signaling can lead to pathological outcomes.

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PKC lambda/iota Antibody (H-12) References:

1. A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle.  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
2. Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC.  |  Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
3. Loss of PKC lambda/iota impairs Th2 establishment and allergic airway inflammation in vivo.  |  Yang, JQ., et al. 2009. Proc Natl Acad Sci U S A. 106: 1099-104. PMID: 19144923
4. Expression and characterization of protein kinase C-delta.  |  Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
5. Echinochrome A Prevents Diabetic Nephropathy by Inhibiting the PKC-Iota Pathway and Enhancing Renal Mitochondrial Function in db/db Mice.  |  Pham, TK., et al. 2023. Mar Drugs. 21: PMID: 37103361
6. Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
7. Turnover of inositol phospholipids and signal transduction.  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
8. The role of protein kinase C in cell surface signal transduction and tumour promotion.  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
9. Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters.  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
10. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651
11. Conventional PKC-alpha, novel PKC-epsilon and PKC-theta, but not atypical PKC-lambda are MARCKS kinases in intact NIH 3T3 fibroblasts.  |  Uberall, F., et al. 1997. J Biol Chem. 272: 4072-8. PMID: 9020116
12. PKC-lambda is the atypical protein kinase C isoform expressed by immature ventricle.  |  Rybin, VO., et al. 1997. Am J Physiol. 272: H1636-42. PMID: 9139945

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PKC lambda/iota Antibody (H-12) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-PKC lambda/iota Antibody (H-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PKC lambda/iota Antibody (H-12) Specifications

Alternate Product Name: PRKCI

Product Clone Name: H-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 168-243

Epitope Target: PKC λ/ι

Epitope Species: human

Product Reactivity/Detection: PKC λ/ι

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for PKC lambda/iota Antibody (H-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PKC antibody is PKC Antibody (A-3): sc-17769.