Date published: 2025-9-8

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Pit-1 抗体 (G-2): sc-25258

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说明书
  • Pit-1 抗体 G-2 是小鼠单克隆 IgG2a κ,Pit-1抗体, 在9篇文献中引用,规格为200 µg/ml
  • 针对全长Pit-1,rat起源
  • Pit-1 抗体 (G-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Pit-1
  • 抗Pit-1抗体(G-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-25258 X, 200 µg/0.1 ml)
  • 关于如何获取Pit-1 (G-2): sc-25258免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP是Pit-1 Antibody (G-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Pit-1 Antibody (G-2) 打包提供(请参阅下面的订购信息)。

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関連項目

Pit-1抗体(G-2)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类的Pit-1蛋白。Pit-1 (G-2) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。Pit-1,也称为生长激素因子-1(GHF-1),是一种重要的转录调节因子,在垂体前叶发育中起着重要作用,GHF-1对于生长激素、催乳素和促甲状腺激素的分化与功能至关重要。Pit-1的特征是具有一个POU结构域,该结构域包含一个与特定基因启动子相互作用的二分体DNA结合结构域,可激活生长激素和催乳素的转录。Pit-1作为基因表达的激活剂和抑制剂的双重作用,维持了激素分泌的平衡,确保了垂体的正常功能。Pit-1的突变可导致垂体激素综合缺乏症(CPHD),影响生长激素、催乳素和促甲状腺激素的分泌,凸显了Pit-1在内分泌健康中的重要性。Pit-1的基因位于人类染色体3p11上,突出了进化保守性和跨物种的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Pit-1 抗体 (G-2) 参考文献:

  1. Pit-1 转录因子的核下分区和功能调控。  |  Mancini, MG., et al. 1999. J Cell Biochem. 72: 322-38. PMID: 10022514
  2. Pit-1 DNA位点对细胞类型规格化中长期抑制的别构效应  |  Scully, KM., et al. 2000. Science. 290: 1127-31. PMID: 11073444
  3. MCF-7 人乳腺癌细胞系中 Pit-1/GHF-1 和 GH 的表达。  |  Gil-Puig, C., et al. 2002. J Endocrinol. 173: 161-7. PMID: 11927395
  4. 一种替代剪接的 Pit-1 异构体改变了其反式激活能力。  |  Morris, AE., et al. 1992. Nucleic Acids Res. 20: 1355-61. PMID: 1561093
  5. 翻译起始位点的替代使用导致 Pit-1 有两种不同的结构形式。  |  Voss, JW., et al. 1991. J Biol Chem. 266: 12832-5. PMID: 2071572
  6. POU结构域:哺乳动物pit-1, oct-1, oct-2和草履虫unc-86基因产物中的一个大的保守区域。  |  Herr, W., et al. 1988. Genes Dev. 2: 1513-6. PMID: 3215510
  7. SGK3通过Pit-1促进慢性肾脏病血管钙化  |  Dong, QQ., et al. 2024. Theranostics. 14: 861-878. PMID: 38169564
  8. Pit-1 在激活和协同作用方面表现出独特的启动子间隔要求。  |  Smith, KP., et al. 1995. J Biol Chem. 270: 4484-91. PMID: 7876215
  9. 中枢性甲状腺功能减退症揭示了Pit-1基因的复合杂合突变。  |  Brown, MR., et al. 1998. Horm Res. 49: 98-102. PMID: 9485179
  10. 垂体前叶的发育和 Pit-1/GHF-1 转录因子。  |  de la Hoya, M., et al. 1998. Cell Mol Life Sci. 54: 1059-66. PMID: 9817984
  11. 编码人类垂体特异性转录因子 Pit-1 基因的特征。  |  Ohta, K., et al. 1992. Gene. 122: 387-8. PMID: 1487156
  12. 神经发育中的 POU 结构域因子  |  Schonemann, MD., et al. 1998. Adv Exp Med Biol. 449: 39-53. PMID: 10026784

订购信息

产品名称产品编号规格价格数量收藏夹

Pit-1 抗体 (G-2)

sc-25258
200 µg/ml
$316.00

Pit-1 (G-2): m-IgG Fc BP-HRP 套装

sc-525511
200 µg Ab; 10 µg BP
$354.00

Pit-1 (G-2): m-IgG2a BP-HRP 套装

sc-546296
200 µg Ab; 10 µg BP
$354.00

Pit-1 抗体 (G-2) X

sc-25258 X
200 µg/0.1 ml
$316.00

Pit-1 抗体 (G-2) AC

sc-25258 AC
500 µg/ml, 25% agarose
$416.00

Pit-1 抗体 (G-2) HRP

sc-25258 HRP
200 µg/ml
$316.00

Pit-1 抗体 (G-2) FITC

sc-25258 FITC
200 µg/ml
$330.00

Pit-1 抗体 (G-2) PE

sc-25258 PE
200 µg/ml
$343.00

Pit-1 抗体 (G-2) Alexa Fluor® 488

sc-25258 AF488
200 µg/ml
$357.00

Pit-1 抗体 (G-2) Alexa Fluor® 546

sc-25258 AF546
200 µg/ml
$357.00

Pit-1 抗体 (G-2) Alexa Fluor® 594

sc-25258 AF594
200 µg/ml
$357.00

Pit-1 抗体 (G-2) Alexa Fluor® 647

sc-25258 AF647
200 µg/ml
$357.00

Pit-1 抗体 (G-2) Alexa Fluor® 680

sc-25258 AF680
200 µg/ml
$357.00

Pit-1 抗体 (G-2) Alexa Fluor® 790

sc-25258 AF790
200 µg/ml
$357.00

Can Pit-1 Antibody (G-2): sc-25258 X detect chicken in ChIP?

Asked by: Ellyse
Thank you for your question. Unfortunately this antibody has not been epitope mapped, so I am not sure if it can or can not detect chicken Pit-1. However, we would be happy to send you a sample of this antibody, sc-25258 if you would like to try it on your chicken samples! Please contact your local distributor, they will be happy to help get you the sample. You can find our distributor contact information here: https://www.scbt.com/scbt/resources/distributors
Answered by: Tech Service
Date published: 2017-06-15

When should the TransCruz reagent sc-25258 X be used instead of the standard form sc-25258?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_8, d_27, h_5CST
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Rated 5 out of 5 by from IMPECABLE!!! muy recomendable!!!! VOLVERIA SIN DUDObtuve facil una buena marca y especìfica de hipòfisis
Date published: 2019-03-14
Rated 1 out of 5 by from Not working with IF on mouse pituitary sectionNot working. Mouse pituitary, PFA fixed 30% sucrose, cryosection.
Date published: 2018-10-10
Rated 5 out of 5 by from Produced positive Western Blot data of PitProduced positive Western Blot data of Pit-1 expression in rat pituitary tissue extract. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from Produced positive Western Blot data of PitProduced positive Western Blot data of Pit-1 expression in non-transfected 293, human Pit-1 transfected 293 and GH3 whole cell lysates. -SCBT QC
Date published: 2014-06-14
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Pit-1 抗体 (G-2) is rated 4.0 out of 5 by 4.
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