Date published: 2025-9-7
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PIF1 Antibody (F-10) - Western Blotting - Image 374822
PIF1 抗体 (F-10): sc-48377. HeLa (A), Jurkat (B), COLO 205 (C), F9 (D), WEHI-231 (E) 和 PC-12 (F) 全细胞裂解液中 PIF1 表达的 Western 印迹分析.
PIF1 抗体 (F-10): sc-48377. HeLa (A), Jurkat (B), COLO 205 (C), F9 (D), WEHI-231 (E) 和 PC-12 (F) 全细胞裂解液中 PIF1 表达的 Western 印迹分析.

PIF1 抗体 (F-10): sc-48377

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说明书
  • PIF1 抗体 F-10 是小鼠单克隆 IgG1 κ，PIF1抗体, 在7篇文献中引用，规格为200 µg/ml
  • 针对全长PIF1，mouse起源
  • PIF1 抗体 (F-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PIF1
  • 抗PIF1抗体（F-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-48377 X, 200 µg/0.1 ml)
  • 关于如何获取PIF1 (F-10): sc-48377的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 PIF1 抗体 (F-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PIF1 抗体 (F-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

PIF1抗体（F-10）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的PIF1蛋白。PIF1 (F-10) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。PIF1是一种单链DNA依赖性ATP酶和解旋酶，能够以5′至3′方向解开DNA，这对于酿酒酵母等生物体的DNA复制和端粒长度维持至关重要。该功能有助于防止基因组不稳定，而基因组不稳定会导致包括癌症在内的各种疾病。PIF1在物种间高度保守，突变会导致端粒延长，表明其对新端粒形成和延长的催化抑制作用。在人类中，PIF1优先结合端粒DNA，抑制端粒酶活性，从而在细胞衰老和肿瘤抑制中发挥重要作用。PIF1可抵消RecQ同源物Sgs1解旋酶的活性，而PIF1的降解则由泛素-26S蛋白酶体途径调节，这凸显了其在维持基因组完整性方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PIF1 抗体 (F-10) 参考文献:

  1. Schizosaccharomyces pombe pfh1+ 编码一种重要的 5' 至 3' DNA 螺旋酶，它是 DNA 螺旋酶 PIF1 亚家族的成员。  |  Zhou, JQ., et al. 2002. Mol Biol Cell. 13: 2180-91. PMID: 12058079
  2. 人类 Pif1 螺旋酶是大肠杆菌 RecD 的潜在同源物，它能抑制端粒酶的活性。  |  Zhang, DH., et al. 2006. Nucleic Acids Res. 34: 1393-404. PMID: 16522649
  3. 有证据表明，Pif1 螺旋酶与 Dna2 螺旋酶/核酸酶和 DNA 聚合酶 delta 一起在 DNA 复制中发挥作用。  |  Budd, ME., et al. 2006. Mol Cell Biol. 26: 2490-500. PMID: 16537895
  4. 重组人 Pif1 的 ATPase 活性特征。  |  Huang, Y., et al. 2006. Acta Biochim Biophys Sin (Shanghai). 38: 335-41. PMID: 16680374
  5. Top3 的缺失揭示了酿酒酵母中 Sgs1 和 Pif1 DNA 螺旋酶之间的相互作用。  |  Wagner, M., et al. 2006. Genetics. 174: 555-73. PMID: 16816432
  6. 类 Pif1 螺旋酶在维持基因组稳定性中的作用  |  Boulé, JB. and Zakian, VA. 2006. Nucleic Acids Res. 34: 4147-53. PMID: 16935874
  7. PIF1：酵母线粒体中的 DNA 螺旋酶。  |  Lahaye, A., et al. 1991. EMBO J. 10: 997-1007. PMID: 1849081
  8. Pif1乙酰化的动态调控对于维持基因组稳定性至关重要。  |  Ononye, OE., et al. 2021. Curr Genet. 67: 85-92. PMID: 33079209
  9. Pif1家族解旋酶在复制叉的作用和调控。  |  Malone, EG., et al. 2022. Int J Mol Sci. 23: PMID: 35409096
  10. 植物色素增强SOS2介导的PIF1和PIF3磷酸化降解，从而促进拟南芥的耐盐性。  |  Ma, L., et al. 2023. Plant Cell. 35: 2997-3020. PMID: 37119239
  11. 来自酿酒酵母的 PIF1 DNA 螺旋酶。该酶的生化特征。  |  Lahaye, A., et al. 1993. J Biol Chem. 268: 26155-61. PMID: 8253734
  12. 糖酵母 PIF1 DNA 螺旋酶抑制端粒的伸长和新端粒的形成。  |  Schulz, VP. and Zakian, VA. 1994. Cell. 76: 145-55. PMID: 8287473

订购信息

产品名称产品编号规格价格数量收藏夹

PIF1 抗体 (F-10)

sc-48377
200 µg/ml
$316.00

PIF1 (F-10): m-IgG Fc BP-HRP 套装

sc-528533
200 µg Ab; 10 µg BP
$354.00

PIF1 (F-10): m-IgGκ BP-HRP 套装

sc-520930
200 µg Ab, 40 µg BP
$354.00

PIF1 抗体 (F-10) X

sc-48377 X
200 µg/0.1 ml
$316.00

PIF1 抗体 (F-10) AC

sc-48377 AC
500 µg/ml, 25% agarose
$416.00

PIF1 抗体 (F-10) HRP

sc-48377 HRP
200 µg/ml
$316.00

PIF1 抗体 (F-10) FITC

sc-48377 FITC
200 µg/ml
$330.00

PIF1 抗体 (F-10) PE

sc-48377 PE
200 µg/ml
$343.00

PIF1 抗体 (F-10) Alexa Fluor® 488

sc-48377 AF488
200 µg/ml
$357.00

PIF1 抗体 (F-10) Alexa Fluor® 546

sc-48377 AF546
200 µg/ml
$357.00

PIF1 抗体 (F-10) Alexa Fluor® 594

sc-48377 AF594
200 µg/ml
$357.00

PIF1 抗体 (F-10) Alexa Fluor® 647

sc-48377 AF647
200 µg/ml
$357.00

PIF1 抗体 (F-10) Alexa Fluor® 680

sc-48377 AF680
200 µg/ml
$357.00

PIF1 抗体 (F-10) Alexa Fluor® 790

sc-48377 AF790
200 µg/ml
$357.00

There are two isoforms of human PIF1 gene product, so does the antibody sc-48377 recognize both isoforms? The reference is listed below. doi: 10.1248/bpb.30.1685. PMID: 17827721.

Asked by: Robert
Thank you for your question. PIF1 Antibody (F-10): sc-48377 has been raised against full length PIF1 of mouse origin. No epitope mapping has been performed as of yet, the exact binding site on its target protein is not known, so we are not sure it can recognize both isoforms. If you have any other questions, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Sen Li
Date published: 2024-07-01

For the Pif1 monoclonal antibody, I would like to know what sequence of the protein does the antibody recognize. Thanks

Asked by: Vaibhavi
Thank you for your question. PIF1 Antibody (F-10): sc-48377 has been raised against full length PIF1 of mouse origin. No epitope mapping has been performed as of yet, so the exact binding site on its target protein is not known.
Answered by: Tech Support Europe
Date published: 2021-02-09

When should the TransCruz reagent sc-48377 X be used instead of the standard form sc-48377?

Asked by: Professor Griffin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good for WBI compared this antibody and another Pif1 antibody from another company. This is much strong and clear than the other. Recomend!
Date published: 2018-02-20
Rated 5 out of 5 by from Great.I bought this antibody two weeks ago. it works very well, very low background.
Date published: 2017-08-23
Rated 5 out of 5 by from IP publishable data with yeast sampleIP publishable data with yeast sample - SCBT Publication Review
Date published: 2015-02-24
Rated 5 out of 5 by from Exceptional immunoperoxidase nuclear stainingExceptional immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human appendix tissue. -SCBT QC
Date published: 2014-05-20
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PIF1 抗体 (F-10) is rated 5.0 out of 5 by 4.
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