PIASy Antibody (F-9): sc-166744
- PIASy Antibody (F-9) is a mouse monoclonal IgG3 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 97-114 near the N-terminus of PIASy of human origin
- recommended for detection of PIASy of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG3 BP-HRP is the preferred secondary detection reagent for PIASy Antibody (F-9) for WB applications. This reagent is now offered in a bundle with PIASy Antibody (F-9) (see ordering information below).
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PIASy Antibody (F-9) is a mouse monoclonal IgG3 antibody that detects PIASy in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PIASy, a member of the protein inhibitor of activated signal transducer and activator of transcription (STAT) family, plays a crucial role in regulating cytokine signaling by acting as a negative regulator of STAT proteins, thereby modulating gene activation, proliferation, and differentiation in response to cytokines. PIASy is primarily located in the nucleus, where PIASy functions as a transcriptional corepressor, particularly for the androgen receptor, and is involved in the regulation of p53-mediated events, which are essential for maintaining cellular homeostasis and apoptosis. PIASy′s ability to influence these critical pathways highlights PIASy′s importance in various physiological processes and potential implications in diseases such as cancer, where dysregulation of these signaling pathways can lead to uncontrolled cell growth. With versatile applications and significant role in cellular signaling, anti-PIASy antibody (F-9) serves as an invaluable tool for researchers studying the intricate mechanisms of cytokine signaling and transcriptional regulation.
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PIASy Antibody (F-9) References:
- PIASy-mediated repression of the androgen receptor is independent of sumoylation. | Gross, M., et al. 2004. Oncogene. 23: 3059-66. PMID: 14981544
- SUMO-1 modification of PIASy, an E3 ligase, is necessary for PIASy-dependent activation of Tcf-4. | Ihara, M., et al. 2005. Mol Cell Biol. 25: 3506-18. PMID: 15831457
- PIASy mediates SUMO-2 conjugation of Topoisomerase-II on mitotic chromosomes. | Azuma, Y., et al. 2005. EMBO J. 24: 2172-82. PMID: 15933717
- PIASy-mediated sumoylation of Yin Yang 1 depends on their interaction but not the RING finger. | Deng, Z., et al. 2007. Mol Cell Biol. 27: 3780-92. PMID: 17353273
- Spatial interplay between PIASy and FIP200 in the regulation of signal transduction and transcriptional activity. | Martin, N., et al. 2008. Mol Cell Biol. 28: 2771-81. PMID: 18285457
- Rod/Zw10 complex is required for PIASy-dependent centromeric SUMOylation. | Ryu, H. and Azuma, Y. 2010. J Biol Chem. 285: 32576-85. PMID: 20696768
- PIASy inhibits virus-induced and interferon-stimulated transcription through distinct mechanisms. | Kubota, T., et al. 2011. J Biol Chem. 286: 8165-8175. PMID: 21199872
- PIASy-mediated Tip60 sumoylation regulates p53-induced autophagy. | Naidu, SR., et al. 2012. Cell Cycle. 11: 2717-28. PMID: 22751435
- PIASy-mediated sumoylation of SREBP1c regulates hepatic lipid metabolism upon fasting signaling. | Lee, GY., et al. 2014. Mol Cell Biol. 34: 926-38. PMID: 24379443
- Downregulation of cardiac PIASy inhibits Cx43 SUMOylation and ameliorates ventricular arrhythmias in a rat model of myocardial ischemia/reperfusion injury. | Wang, T., et al. 2023. Chin Med J (Engl). 136: 1349-1357. PMID: 37014755
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIASy Antibody (F-9) | sc-166744 | 200 µg/ml | $322.00 | |||
PIASy Antibody (F-9): m-IgG3 BP-HRP Bundle | sc-550394 | 200 µg Ab; 40 µg BP | $361.00 | |||
PIASy (F-9) Neutralizing Peptide | sc-166744 P | 100 µg/0.5 ml | $69.00 |