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PGK1 Antibody (14) - Western Blotting - Image 390982
PGK1 抗体 (14) HRP: sc-130335 HRP. 直接对 JAR (A), Hep G2 (B), NCI-H1299 (C), A-431 (D), Caco-2 (E) 和 HeLa (F) 全细胞裂解液中 PGK1 的表达进行 western 印迹分析.
PGK1 抗体 (14) HRP: sc-130335 HRP. 直接对 JAR (A), Hep G2 (B), NCI-H1299 (C), A-431 (D), Caco-2 (E) 和 HeLa (F) 全细胞裂解液中 PGK1 的表达进行 western 印迹分析.

PGK1 抗体 (14): sc-130335

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寫評論提問

说明书
  • PGK1 抗体 14 是小鼠单克隆 IgG1 κ，PGK1抗体, 在37篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组PGK1
  • PGK1 抗体 (14) 推荐用于 WB, IP, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PGK1
  • 抗PGK1抗体（14）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PGK1 (14): sc-130335的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是PGK1 Antibody (14) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与PGK1 Antibody (14) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外PGK抗体，包括PGK2

PGK1抗体（14）是一种IgG1 κ小鼠单克隆PGK1抗体（也称为PGK-1抗体、细胞迁移诱导基因10蛋白抗体、MIG10抗体、引物识别蛋白2抗体、PRP 2抗体或PGKA抗体），它通过WB、IP、IHC(P)和ELISA检测小鼠、大鼠和人源的PGK1蛋白。PGK1抗体（14）既可作为非偶联的抗PGK1抗体形式提供，也可作为多种偶联的抗PGK1抗体形式提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。磷酸甘油酸激酶1/2（PGK1/2，ATP：3-磷酸-D-甘油酸1-磷酸转移酶，EC 2.7.2.3）是体细胞表达的糖酵解酶，催化1，3-双磷酸甘油酸的酰基磷酸向ADP的磷酸基团转移，从而形成ATP和3-磷酸甘油酸。人类PGK基因被10个内含子中断，跨越23个千碱基，并与X染色体上的Xq21.1位置相连，该区域与前列腺癌、雄激素不敏感、会阴尿道下裂和其他遗传异常有关。除了影响糖酵解外，PGK1还由肿瘤细胞分泌，并作为二硫键还原酶促进增殖性血管生成过程。PGK1介导的丝氨酸蛋白酶纤溶酶中二硫键的减少启动了肿瘤血管抑制剂血管抑素的释放，这是肿瘤扩张和转移中血管形成或血管生成的关键事件。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PGK1 抗体 (14) 参考文献:

  1. 人类磷酸甘油酸激酶基因 3' 处的半杂合短串联重复多态性。  |  Riley, DE., et al. 1999. Mol Biol Rep. 26: 159-65. PMID: 10532310
  2. 细胞糖酵解酶磷酸甘油酸激酶参与仙台病毒的转录。  |  Ogino, T., et al. 1999. J Biol Chem. 274: 35999-6008. PMID: 10585490
  3. 磷酸甘油酸激酶在肿瘤血管生成过程中充当二硫化物还原酶。  |  Lay, AJ., et al. 2000. Nature. 408: 869-73. PMID: 11130727
  4. 人类磷酸甘油酸激酶基因的结构及内含子介导的核苷酸结合域的进化和分散。  |  Michelson, AM., et al. 1985. Proc Natl Acad Sci U S A. 82: 6965-9. PMID: 2995995
  5. 巨噬细胞相关的 PGK1 磷酸化促进有氧糖酵解和肿瘤发生  |  Zhang, Y., et al. 2018. Mol Cell. 71: 201-215.e7. PMID: 30029001
  6. PTEN 通过去磷酸化和抑制自磷酸化 PGK1 来抑制糖酵解。  |  Qian, X., et al. 2019. Mol Cell. 76: 516-527.e7. PMID: 31492635
  7. PGK1的定位决定了SMAD4阴性胰腺癌患者平衡转移和增殖的代谢表型。  |  Liang, C., et al. 2020. Gut. 69: 888-900. PMID: 31611300
  8. PGK1 的 O-GlcNAcylation 可协调糖酵解和 TCA 循环，促进肿瘤生长。  |  Nie, H., et al. 2020. Nat Commun. 11: 36. PMID: 31911580
  9. PGK1羰基化在缺氧条件下下调，通过协调糖酵解和TCA循环促进肿瘤发生。  |  Chu, Z., et al. 2021. Mol Ther. 29: 2737-2753. PMID: 33940159
  10. FOXO3A诱导的LINC00926通过抑制PGK1介导的Warburg效应抑制乳腺癌的生长和转移。  |  Liang, J., et al. 2022. J Transl Med. 20: 80. PMID: 35123484
  11. HIF1α/ATF3通过调节胶质母细胞瘤中的P4HA1/琥珀酸信号传导，参与PGK1 K191/K192琥珀酸化。  |  Yang, S., et al. 2024. Neuro Oncol. 26: 1405-1420. PMID: 38441561
  12. 磷酸甘油酸激酶1（PGK1）在癌症中的诊断和治疗前景广阔。  |  Guo, Z., et al. 2024. Nat Commun. 15: 6915. PMID: 39134530

订购信息

产品名称产品编号规格价格数量收藏夹

PGK1 抗体 (14)

sc-130335
200 µg/ml
$316.00

PGK1 (14): m-IgG Fc BP-HRP 套装

sc-528731
200 µg Ab; 10 µg BP
$354.00

PGK1 (14): m-IgGκ BP-HRP 套装

sc-521211
200 µg Ab, 40 µg BP
$354.00

PGK1 (14): m-IgG1 BP-HRP 套装

sc-543099
200 µg Ab; 20 µg BP
$354.00

PGK1 抗体 (14) AC

sc-130335 AC
500 µg/ml, 25% agarose
$416.00

PGK1 抗体 (14) HRP

sc-130335 HRP
200 µg/ml
$316.00

PGK1 抗体 (14) FITC

sc-130335 FITC
200 µg/ml
$330.00

PGK1 抗体 (14) PE

sc-130335 PE
200 µg/ml
$343.00

PGK1 抗体 (14) Alexa Fluor® 488

sc-130335 AF488
200 µg/ml
$357.00

PGK1 抗体 (14) Alexa Fluor® 546

sc-130335 AF546
200 µg/ml
$357.00

PGK1 抗体 (14) Alexa Fluor® 594

sc-130335 AF594
200 µg/ml
$357.00

PGK1 抗体 (14) Alexa Fluor® 647

sc-130335 AF647
200 µg/ml
$357.00

PGK1 抗体 (14) Alexa Fluor® 680

sc-130335 AF680
200 µg/ml
$357.00

PGK1 抗体 (14) Alexa Fluor® 790

sc-130335 AF790
200 µg/ml
$357.00

I am using PGK1 (14): sc-130335 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24

Hey, we want to know the exact antigen sequence of PGK1 your antibody detected. We have constructed some truncations of PGK1 and also want to use your antibody. Thanks a lot.

Asked by: naiyang
  • y_2025, m_8, d_27, h_4CST
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Rated 5 out of 5 by from Cross reacts with budding yeast lysateCross reacted with a protein at the expected size from TCA precipitated whole cell lysate from budding yeast S. cerevisiae. 10% SDS-PAGE gel, antibody used at 1:1000
Date published: 2017-10-23
Rated 5 out of 5 by from Works great at ratio 1:500PGK1 Antibody Works great at ratio 1:500. Only one specific protein band was detected!
Date published: 2017-08-30
Rated 5 out of 5 by from VERY GOOD ANTIBODYWorks great at ratio 1:500; Blocking: 5% milk in TBST; First Ab: 1:500 5% milk in TBST; Second Ab: 1:5000 5% milk in TBST;
Date published: 2017-08-01
Rated 5 out of 5 by from Very good antibodyA very strong band at the right size, recommended to use.
Date published: 2017-07-15
Rated 5 out of 5 by from Excellent choice for PGK1!Great antibody for Western blot, beautiful strong bands in human and mouse samples. Also gives specific and clean staining for IHC. Great choice!
Date published: 2017-01-23
Rated 4 out of 5 by from Wonderful immunoperoxidase cytoplasmic andWonderful immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human thyroid gland tissue. -SCBT QC
Date published: 2015-04-15
Rated 5 out of 5 by from Produced a clear band with HeLa whole cell lysateProduced a clear band with HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-05
Rated 4 out of 5 by from WB publishable data with human breast tumorWB publishable data with human breast tumor tissues, AsPC-1 and BxPC-3 cell lysates - SCBT Publication Review
Date published: 2014-04-23
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PGK1 抗体 (14) is rated 4.8 out of 5 by 8.
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