Date published: 2025-9-8
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PGK1/2 Antibody (A-5) - Western Blotting - Image 359648
PGK1/2 抗体 (A-5): sc-48342. JAR (A), HeLa (B), Hep G2 (C) 和 A549 (D) 全细胞裂解液中 PGK1/2 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
PGK1/2 抗体 (A-5): sc-48342. JAR (A), HeLa (B), Hep G2 (C) 和 A549 (D) 全细胞裂解液中 PGK1/2 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

PGK1/2 抗体 (A-5): sc-48342

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寫評論提問

说明书
  • PGK1/2 抗体 A-5 是小鼠单克隆 IgG1 κ，PGK1/2抗体, 在18篇文献中引用，规格为200 µg/ml
  • 免疫human来源PGK1位于C-terminus 的119-418氨基酸
  • PGK1/2 抗体 (A-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PGK1 and PGK2
  • 抗PGK1/2抗体（A-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PGK1/2 (A-5): sc-48342的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 PGK1/2 抗体 (A-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PGK1/2 抗体 (A-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 PGK 抗体 包括 PGK2

PGK1/2抗体（A-5）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的PGK1/2蛋白。PGK1/2 (A-5) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。磷酸甘油酸激酶1和2（PGK1/2）是重要的糖酵解酶，在细胞能量代谢中起着重要作用，它们催化1,3-二磷酸甘油酸上的磷酰基转移到二磷酸腺苷（ADP）上，从而产生三磷酸腺苷（ATP）和3-磷酸甘油酸。PGK1由肿瘤细胞分泌，参与血管生成，即新血管的形成过程，这对肿瘤的生长和转移至关重要。PGK1是一种二硫键还原酶，可促进丝氨酸蛋白酶纤溶酶中的二硫键还原，从而触发血管抑制素释放，后者是肿瘤血管形成的强效抑制剂。人类PGK基因位于X染色体上，跨越23个千碱基对，被10个内含子打断，该区域的突变与多种遗传疾病有关，包括前列腺癌和雄激素不敏感症。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PGK1/2 抗体 (A-5) 参考文献:

  1. 人类磷酸甘油酸激酶基因 3' 处的半杂合短串联重复多态性。  |  Riley, DE., et al. 1999. Mol Biol Rep. 26: 159-65. PMID: 10532310
  2. 细胞糖酵解酶磷酸甘油酸激酶参与仙台病毒的转录。  |  Ogino, T., et al. 1999. J Biol Chem. 274: 35999-6008. PMID: 10585490
  3. 磷酸甘油酸激酶在肿瘤血管生成过程中充当二硫化物还原酶。  |  Lay, AJ., et al. 2000. Nature. 408: 869-73. PMID: 11130727
  4. 人类磷酸甘油酸激酶1（PGK1）的过表达会诱导多药耐药表型。  |  Duan, Z., et al. 2002. Anticancer Res. 22: 1933-41. PMID: 12174867
  5. 糖酵解酶 3-磷酸甘油酸激酶在活化 L-核苷类似物（一类新型抗癌和抗病毒药物）中的新作用。  |  Krishnan, P., et al. 2003. J Biol Chem. 278: 36726-32. PMID: 12869554
  6. 肿瘤细胞分泌的磷酸甘油酸激酶受氧感应羟化酶控制。  |  Daly, EB., et al. 2004. Biochim Biophys Acta. 1691: 17-22. PMID: 15053920
  7. 人类磷酸甘油酸激酶基因的结构及内含子介导的核苷酸结合域的进化和分散。  |  Michelson, AM., et al. 1985. Proc Natl Acad Sci U S A. 82: 6965-9. PMID: 2995995

订购信息

产品名称产品编号规格价格数量收藏夹

PGK1/2 抗体 (A-5)

sc-48342
200 µg/ml
$316.00

PGK1/2 (A-5): m-IgG Fc BP-HRP 套装

sc-528518
200 µg Ab; 10 µg BP
$354.00

PGK1/2 (A-5): m-IgGκ BP-HRP 套装

sc-520914
200 µg Ab, 40 µg BP
$354.00

PGK1/2 (A-5): m-IgG1 BP-HRP 套装

sc-542973
200 µg Ab; 20 µg BP
$354.00

PGK1/2 抗体 (A-5) AC

sc-48342 AC
500 µg/ml, 25% agarose
$416.00

PGK1/2 抗体 (A-5) HRP

sc-48342 HRP
200 µg/ml
$316.00

PGK1/2 抗体 (A-5) FITC

sc-48342 FITC
200 µg/ml
$330.00

PGK1/2 抗体 (A-5) PE

sc-48342 PE
200 µg/ml
$343.00

PGK1/2 抗体 (A-5) Alexa Fluor® 488

sc-48342 AF488
200 µg/ml
$357.00

PGK1/2 抗体 (A-5) Alexa Fluor® 546

sc-48342 AF546
200 µg/ml
$357.00

PGK1/2 抗体 (A-5) Alexa Fluor® 594

sc-48342 AF594
200 µg/ml
$357.00

PGK1/2 抗体 (A-5) Alexa Fluor® 647

sc-48342 AF647
200 µg/ml
$357.00

PGK1/2 抗体 (A-5) Alexa Fluor® 680

sc-48342 AF680
200 µg/ml
$357.00

PGK1/2 抗体 (A-5) Alexa Fluor® 790

sc-48342 AF790
200 µg/ml
$357.00

I am using PGK1/2 (A-5): sc-48342 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Cross reacts with budding yeast cell lysateWorked well against total yeast cell (S. cerevisiae) lysate prepared by TCA precipitation.
Date published: 2018-02-12
Rated 5 out of 5 by from excellent for WBThe antibody works very well to detect endogenous PGK1/2 in cell lysate made from human and mouse cells.
Date published: 2017-09-19
Rated 5 out of 5 by from Wonderful antibodyThis antibody works great at 1:1000 dilution on whole cell lysates! (15 ug protein per well)
Date published: 2017-05-04
Rated 5 out of 5 by from Single strong band by WB in ARPE-19 cells1:1000 dilution, ~25 ug spl, imaged with LI-COR secondary. Single, strong band.
Date published: 2017-04-28
Rated 5 out of 5 by from Excellent productThe PGK1/2 Antibody (A-5): sc-48342 works well for IHC applications when using formalin fixed, paraffin-embedded tissue sections, showing staining pattern localized to the nucleus and cytoplasm. This antibody has also been cited several times for further verification.
Date published: 2017-01-27
Rated 4 out of 5 by from PGK/12 reviewI stained primate trabecular meshwork and that gave me good signal compared to just secondary Antibody control. However my positive control, mouse colon cancer tumor, I had staining with secondary only and primary and secondary. it may be that my peroxide was old. But it seems to work fine in trabecular meshwork
Date published: 2017-01-24
Rated 5 out of 5 by from These antibodies have worked well in WBThese antibodies have worked well in WB
Date published: 2015-10-27
Rated 4 out of 5 by from Strong bands seen in WB using JARStrong bands seen in WB using JAR, JEG-3 and F9 whole cell lysates. -SCBT QC
Date published: 2015-06-16
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PGK1/2 抗体 (A-5) is rated 4.6 out of 5 by 12.
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