Date published: 2025-9-6

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PFKP 抗体 (F-7): sc-514824

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说明书
  • PFKP 抗体 F-7 是小鼠单克隆 IgG1 κ,PFKP抗体, 在10篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列131-174位于human物种的PFKP的内部区域内
  • PFKP 抗体 (F-7) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 PFKP
  • 抗PFKP抗体(F-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PFKP (F-7): sc-514824免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是PFKP Antibody (F-7) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与PFKP Antibody (F-7) 打包提供(请参阅下面的订购信息)。

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関連項目

PFKP抗体(F-7)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的PFKP蛋白。抗 -PFKP抗体(F-7)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。PFKP,也称为磷酸果糖激酶血小板,是糖酵解途径的关键组成部分,作为一种变构酶,它催化果糖-6-磷酸转化为果糖-1,6-二磷酸,这是葡萄糖代谢的关键调节步骤。PFKP主要位于血小板中,在能量产生和代谢调节中发挥着重要作用,特别是在能量需求增加的时期。PFKP的正常运作对于维持细胞能量平衡至关重要,PFKP基因的任何缺陷都可能导致代谢紊乱,包括增加肥胖的风险。在研究中使用PFKP(F-7)单克隆抗体可以深入了解PFKP在各种生理和病理过程中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PFKP 抗体 (F-7) 参考文献:

  1. 用于综合检测和识别复杂蛋白质组中酶活性的三功能化学探针。  |  Adam, GC., et al. 2002. Mol Cell Proteomics. 1: 828-35. PMID: 12438565
  2. 6- 磷酸果糖-2-激酶/果糖-2,6-二磷酸酶-3 基因(PFKFB3,iPFK2)的克隆和染色体特征。  |  Mahlknecht, U., et al. 2003. Int J Oncol. 23: 883-91. PMID: 12963966
  3. 神经细胞系中人类 P 型 6-磷酸果糖-1-激酶基因启动子的特征。  |  Hannemann, A., et al. 2005. Gene. 345: 237-47. PMID: 15716112
  4. 涉及哺乳动物同工酶 N 端和 C 端半部分交换的嵌合磷酸果激酶:对配体结合位点的影响。  |  Martínez-Costa, OH., et al. 2007. FEBS Lett. 581: 3033-8. PMID: 17544406
  5. 编码人血小板磷酸果激酶的 cDNA 的分离和序列。  |  Simpson, CJ. and Fothergill-Gilmore, LA. 1991. Biochem Biophys Res Commun. 180: 197-203. PMID: 1834056
  6. 在对 18,014 名丹麦人的研究中,INSIG2 和 PFKP 的常见变异与肥胖之间基于全基因组的缔合作用未被复制。  |  Andreasen, CH., et al. 2008. PLoS One. 3: e2872. PMID: 18682847
  7. 克隆人肌磷酸果激酶 cDNA。  |  Nakajima, H., et al. 1987. FEBS Lett. 223: 113-6. PMID: 2822475
  8. 乳腺癌中血小板型磷酸果糖激酶(PFKP)的预后和治疗相关性。  |  Umar, SM., et al. 2020. Exp Cell Res. 396: 112282. PMID: 32919954
  9. p53反应性CMBL通过破坏磷酸果糖激酶PFKP的稳定性来重新编程葡萄糖代谢并抑制癌症发展。  |  Huang, Y., et al. 2023. Cell Rep. 42: 113426. PMID: 37967006
  10. 肾小管上皮细胞中PFKP的抑制作用可抑制TGF-β诱导的糖酵解和肾纤维化。  |  Yang, S., et al. 2023. Cell Death Dis. 14: 816. PMID: 38086793
  11. 蛋白质组学分析鉴定出SW480结肠癌细胞中PFKP的乳酸化。  |  Cheng, Z., et al. 2024. iScience. 27: 108645. PMID: 38155775
  12. 人类磷酸果激酶的等位酶:一个新系统的鉴定和亚基结构特征。  |  Vora, S., et al. 1980. Proc Natl Acad Sci U S A. 77: 62-6. PMID: 6444721

订购信息

产品名称产品编号规格价格数量收藏夹

PFKP 抗体 (F-7)

sc-514824
200 µg/ml
$316.00

PFKP (F-7): m-IgG1 BP-HRP 套装

sc-531792
200 µg Ab; 20 µg BP
$354.00

PFKP 抗体 (F-7) AC

sc-514824 AC
500 µg/ml, 25% agarose
$416.00

PFKP 抗体 (F-7) HRP

sc-514824 HRP
200 µg/ml
$316.00

PFKP 抗体 (F-7) FITC

sc-514824 FITC
200 µg/ml
$330.00

PFKP 抗体 (F-7) PE

sc-514824 PE
200 µg/ml
$343.00

PFKP 抗体 (F-7) Alexa Fluor® 488

sc-514824 AF488
200 µg/ml
$357.00

PFKP 抗体 (F-7) Alexa Fluor® 546

sc-514824 AF546
200 µg/ml
$357.00

PFKP 抗体 (F-7) Alexa Fluor® 594

sc-514824 AF594
200 µg/ml
$357.00

PFKP 抗体 (F-7) Alexa Fluor® 647

sc-514824 AF647
200 µg/ml
$357.00

PFKP 抗体 (F-7) Alexa Fluor® 680

sc-514824 AF680
200 µg/ml
$357.00

PFKP 抗体 (F-7) Alexa Fluor® 790

sc-514824 AF790
200 µg/ml
$357.00

I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-514824: PFKP (F-7) mouse monoclonal antibody will work?

Asked by: Dr Ninau Qelp
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Very goodGood signal in Western Blot. PFKP Antibody (F-7): sc-514824 was used in a Western blot analysis of PFKP in nuclear protein extracts of a human cell line with a clean and strong signal. Dilution 1/200.
Date published: 2021-05-26
Rated 5 out of 5 by from great signal in western blottingI obtained a very strong and specific signal using a 1:500 dilution in a number of cancer cell lines
Date published: 2019-06-27
Rated 3 out of 5 by from Works but also binds unspecificallyI have used this antibody on platelets, 1:1000 dilution. It's about 86Kd, but around 63-63kd, there is unspecific binding...
Date published: 2018-03-22
Rated 3 out of 5 by from With a 1:100 dilution rateI used this antibody to detet the Pfkp protin in L929 cell line, and found that, this antibody worked only in 1:100 dilution rate and in a long exposure condition.
Date published: 2017-10-27
Rated 5 out of 5 by from goo WB bandused at 1:200 dilution to detect the expression of PFKP in human cancer cell lines U-2 OS and 1299. The target band is clear.
Date published: 2017-08-29
Rated 5 out of 5 by from WBRight size WB band exists in human macrophage sapmles, and the expression pattern in different kinds of macrophages is as expected.
Date published: 2016-12-24
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PFKP 抗体 (F-7) is rated 4.3 out of 5 by 6.
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