Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Perilipin CRISPR/Cas9 KO Plasmid (h): sc-401305
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Perilipin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Perilipin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Perilipin Antibody (G-2): sc-390169
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Perilipin CRISPR/Cas9 KO Plasmid (h) | sc-401305 | 20 µg | $397.00 |
Overview
PLIN1 encodes perilipin-1, a lipid droplet–associated phosphoprotein that regulates adipocyte triglyceride storage and lipolysis by controlling access of lipases to the droplet surface. Perilipin integrates cAMP/PKA-dependent phosphorylation events with adipose lipid mobilization, coordinating interactions with cofactors such as ABHD5/CGI-58 and enzymes including ATGL and HSL. Through its role in lipid droplet biogenesis and turnover, PLIN1 influences cellular energy homeostasis, fatty acid flux, and adipokine-linked metabolic signaling. Genetic and functional alterations in PLIN1 have been associated with human adipose dysfunction, including lipodystrophy phenotypes and metabolic dysregulation, making it a useful target for studying lipid handling in relevant cell models.
Perilipin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PLIN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PLIN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PLIN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Perilipin protein expression.
This CRISPR knockout system enables efficient generation of PLIN1-deficient cell models for investigation of Perilipin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PLIN1 exon(s) critical for Perilipin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PLIN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Perilipin CRISPR/Cas9 KO Plasmid (h) and Perilipin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PLIN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Perilipin HDR Plasmid (h) and Perilipin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PLIN1 homology arms to support homology-directed repair at defined PLIN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.