Date published: 2025-9-9

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Perilipin 抗体 (G-2): sc-390169

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说明书
  • Perilipin 抗体 G-2 是小鼠单克隆 IgG1 κ,Perilipin抗体, 在21篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的Perilipin的N-terminus
  • Perilipin 抗体 (G-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 Perilipin
  • 抗Perilipin抗体(G-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Perilipin (G-2): sc-390169免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 Perilipin 抗体 (G-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Perilipin 抗体 (G-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Perilipin抗体(G-2)是一种IgG1 κ小鼠单克隆Perilipin抗体(也被称为Perilipin 1抗体、脂质滴相关蛋白抗体、Perilipin-1抗体、PERI抗体、PLIN抗体或FPLD4抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人源的Perilipin蛋白。Perilipin抗体(G-2)既有非偶联型抗Perilipin抗体形式,也有多种偶联形式的抗Perilipin抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。PAT(Perilipin、adipophilin、TIP47)家族蛋白是与脂质滴相关的进化相关蛋白,参与细胞内脂质代谢。磷蛋白Perilipin(Peri),也被称为脂质滴相关蛋白,属于Perilipin亚家族蛋白。Perilipin定位于脂肪细胞内脂质滴表面,通过在脂肪细胞内包覆脂质储存滴来保护它们,直到它们被激素敏感脂肪酶(HSL)消化,从而调节脂肪细胞的脂质代谢。作为脂解的关键调节因子,Perilipin水平升高与肥胖有关,因为其缺失会导致瘦弱。在磷酸化状态下,Perilipin对HSL的敏感性最高。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Perilipin 抗体 (G-2) 参考文献:

  1. 过脂素 a 在脂质代谢和脂肪细胞脂肪分解中的核心作用  |  Tansey, JT., et al. 2004. IUBMB Life. 56: 379-85. PMID: 15545214
  2. 白种人围脂素基因单倍型与肥胖风险的性别相关性。  |  Qi, L., et al. 2004. Obes Res. 12: 1758-65. PMID: 15601970
  3. [Natriuretic peptides:人类脂肪细胞中新的脂肪分解途径]。  |  Sengenes, C., et al. 2005. Med Sci (Paris). 21: 61-5. PMID: 15639022
  4. 人胎膜中脂肪嗜蛋白和过脂蛋白的表达和定位:与脂质体和参与前列腺素合成的酶有关。  |  Meadows, JW., et al. 2005. J Clin Endocrinol Metab. 90: 2344-50. PMID: 15657374
  5. S3-12, Adipophilin 和 TIP47 在脂肪细胞中包装脂质。  |  Wolins, NE., et al. 2005. J Biol Chem. 280: 19146-55. PMID: 15731108
  6. 人体免疫缺陷病毒蛋白酶抑制剂利托那韦对基础脂肪分解和儿茶酚胺刺激脂肪分解的影响。  |  Adler-Wailes, DC., et al. 2005. J Clin Endocrinol Metab. 90: 3251-61. PMID: 15741249
  7. PAT 家族蛋白遍布脂滴核心。  |  Robenek, H., et al. 2005. J Lipid Res. 46: 1331-8. PMID: 15741656
  8. 人类周脂素基因(PLIN)的基因内连锁不平衡结构以及单倍型与多种族亚裔人群肥胖风险增加的关系。  |  Qi, L., et al. 2005. J Mol Med (Berl). 83: 448-56. PMID: 15770500

订购信息

产品名称产品编号规格价格数量收藏夹

Perilipin 抗体 (G-2)

sc-390169
200 µg/ml
$316.00

Perilipin (G-2): m-IgG1 BP-HRP 套装

sc-531736
200 µg Ab; 20 µg BP
$354.00

Perilipin 抗体 (G-2) AC

sc-390169 AC
500 µg/ml, 25% agarose
$416.00

Perilipin 抗体 (G-2) HRP

sc-390169 HRP
200 µg/ml
$316.00

Perilipin 抗体 (G-2) FITC

sc-390169 FITC
200 µg/ml
$330.00

Perilipin 抗体 (G-2) PE

sc-390169 PE
200 µg/ml
$343.00

Perilipin 抗体 (G-2) Alexa Fluor® 488

sc-390169 AF488
200 µg/ml
$357.00

Perilipin 抗体 (G-2) Alexa Fluor® 546

sc-390169 AF546
200 µg/ml
$357.00

Perilipin 抗体 (G-2) Alexa Fluor® 594

sc-390169 AF594
200 µg/ml
$357.00

Perilipin 抗体 (G-2) Alexa Fluor® 647

sc-390169 AF647
200 µg/ml
$357.00

Perilipin 抗体 (G-2) Alexa Fluor® 680

sc-390169 AF680
200 µg/ml
$357.00

Perilipin 抗体 (G-2) Alexa Fluor® 790

sc-390169 AF790
200 µg/ml
$357.00

Could this antibody be used in a Flow Cytometry application?

Asked by: Matt
Thank you for your question. Yes, we warranty the antibody for Flow CytometryWB, IP, PAR, and ELISA. Here is our Flow Cytometry protocol :https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-10-02

Do this antibody recognize perilipin 1 in adipocytes?

Asked by: MSSMRK
Thank you for your question. Perilipin Antibody (G-2) is recommended for detection of Perilipin of human origin by WB, IP, IF, IHC(P) and ELISA and will also detect its target in human adipocytes when used in the listed applications.
Answered by: Tech Support Europe
Date published: 2023-03-01

Is (sc-390169) Perilipin antibody able to be conducted in rat-sourced cells ?

Asked by: Jamie920921
Thank you for the question. This antibody is recommended for detection of Perilipin of human origin by WB, IP, IF, IHC(P) and ELISA. We have not fully evaluated this antibody with Perilipin of mouse or rat origin. However, we suspect the reactivity will not be very strong and thus we do not recommend this antibody for the detection of Perilipin of rat origin.
Answered by: Technical Support Europe
Date published: 2017-12-03

I am using Perilipin (G-2): sc-390169 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_28, h_5CST
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Rated 5 out of 5 by from Ottimo funzionamentoAnticorpo specifico su sezioni di muscolo congelate e su cellule in coltura fissate in metanolo a freddo. Ottimo risultato
Date published: 2021-11-10
Rated 5 out of 5 by from Excellent featuresI brought this a month ago and pretty happy with it
Date published: 2020-11-21
Rated 5 out of 5 by from Good Ab for IFThis antibody is good for immunofluorescent staining (1:50 in 1%BSA in PBS). Highly recommended!
Date published: 2018-11-13
Rated 5 out of 5 by from Worked GreatThis antibody worked for a Western blot the first time I tried it in rat white adipose tissue.
Date published: 2018-02-21
Rated 4 out of 5 by from IFA可以用间接免疫荧光检测可以用,WB以及其他方面还没有验证。待验证后补充评论。总体效果较好~~~~~~~~~
Date published: 2018-01-02
Rated 4 out of 5 by from Surprise2017-10-14 293T proteins:30ug per lane. Blocking buffer:5% nonfat dry milk in TBST for 2 hours. primary antibody:Perilipin Antibody (G-2) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Mouse IgG (ab6789) at 1:20000 dilution. Exposure time:9 seconds. Visual molecular weight:57KD,62KD(See Figure 1) 2017-10-16 293T proteins:30ug per lane. Blocking buffer:5% nonfat dry milk in TBST for 4 hours. primary antibody:Perilipin Antibody (G-2) at 1:800 dilution. Secondary antibody:HRP Goat Anti-Mouse IgG (ab6789) at 1:20000 dilution. Exposure time:20 seconds. Visual molecular weight:57KD(See Figure 2) 2017-10-16 rat BMSC proteins:20ug per lane. Blocking buffer:5% nonfat dry milk in TBST for 4 hours. primary antibody:Perilipin Antibody (G-2) at 1:800 dilution. Secondary antibody:HRP Goat Anti-Mouse IgG (ab6789) at 1:20000 dilution. Exposure time:1 minutes. Visual molecular weight:62KD(See Figure 3)
Date published: 2017-10-17
Rated 3 out of 5 by from not too badI got a band in my western blot but not good that much
Date published: 2017-08-09
Rated 4 out of 5 by from It is ok for IF1:100, I can detect perilipin for IF. But I didnt test it for WB
Date published: 2017-07-04
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Perilipin 抗体 (G-2) is rated 4.5 out of 5 by 10.
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