CD31/PECAM-1 Antibody (10G9): sc-13537

PECAM-1 Antibody (10G9) is a mouse monoclonal IgG1 kappa light chain antibody that detects human PECAM-1 by immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-PECAM-1 antibody (10G9) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PECAM-1, also known as CD31, is a crucial glycoprotein expressed on monocytes, neutrophils, platelets, and a subset of T cells, playing a vital role in mediating cell-cell adhesion and signaling during inflammatory responses and angiogenesis. PECAM-1 is predominantly located at intercellular junctions of endothelial cells, where PECAM-1 facilitates leukocyte migration across vascular endothelium, a process essential for immune surveillance and tissue repair. Understanding PECAM-1 localization and function is important, as PECAM-1 dysregulation can contribute to various pathological conditions, including chronic inflammation and tumor metastasis. PECAM-1 interaction with other adhesion molecules, such as integrins, further underscores PECAM-1′s significance in maintaining vascular integrity and regulating immune responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD31/PECAM-1 Antibody (10G9) References:

1. Mechanisms of PECAM-1-mediated cytoprotection and implications for cancer cell survival.  |  Bergom, C., et al. 2005. Leuk Lymphoma. 46: 1409-21. PMID: 16194886
2. PECAM-1: a multi-functional molecule in inflammation and vascular biology.  |  Woodfin, A., et al. 2007. Arterioscler Thromb Vasc Biol. 27: 2514-23. PMID: 17872453
3. PECAM-1: conflicts of interest in inflammation.  |  Privratsky, JR., et al. 2010. Life Sci. 87: 69-82. PMID: 20541560
4. PECAM-1: regulator of endothelial junctional integrity.  |  Privratsky, JR. and Newman, PJ. 2014. Cell Tissue Res. 355: 607-19. PMID: 24435645
5. Endothelial PECAM-1 and its function in vascular physiology and atherogenic pathology.  |  Chistiakov, DA., et al. 2016. Exp Mol Pathol. 100: 409-15. PMID: 27079772
6. Roles of PECAM-1 in cell function and disease progression.  |  Feng, YM., et al. 2016. Eur Rev Med Pharmacol Sci. 20: 4082-4088. PMID: 27775789
7. Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination.  |  Abraham, V., et al. 2018. Int J Oncol. 53: 488-502. PMID: 29845213
8. Generation of PECAM-1 (CD31) conditional knockout mice.  |  Zhi, H., et al. 2020. Genesis. 58: e23346. PMID: 31729819
9. PECAM-1 protects against DIC by dampening inflammatory responses via inhibiting macrophage pyroptosis and restoring vascular barrier integrity.  |  Luo, L., et al. 2020. Transl Res. 222: 1-16. PMID: 32417429
10. PECAM-1 drives β-catenin-mediated EndMT via internalization in colon cancer with diabetes mellitus.  |  Wu, Q., et al. 2023. Cell Commun Signal. 21: 203. PMID: 37580771
11. The role of PECAM-1 (CD31) in leukocyte emigration: studies in vitro and in vivo.  |  Muller, WA. 1995. J Leukoc Biol. 57: 523-8. PMID: 7722409
12. Thrombospondin-1, PECAM-1, and regulation of angiogenesis.  |  Sheibani, N. and Frazier, WA. 1999. Histol Histopathol. 14: 285-94. PMID: 9987673

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD31/PECAM-1 Antibody (10G9) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-CD31/PECAM-1 Antibody (10G9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD31/PECAM-1 Antibody (10G9) Specifications

Alternate Product Name: CD31

Product Clone Name: 10G9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: PECAM-1

Epitope Species: human

Product Reactivity/Detection: PECAM-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for CD31/PECAM-1 Antibody (10G9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD31/PECAM-1 antibody is CD31/PECAM-1 Antibody (MEC 13.3): sc-18916.