PDK2 Antibody (180CT10.2.3): sc-517351

PDK2 Antibody (180CT10.2.3) is a mouse monoclonal IgG1 antibody that detects pyruvate dehydrogenase kinase 2 (PDK2) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. PDK2 plays a crucial role in cellular metabolism by regulating the activity of the pyruvate dehydrogenase complex (PDC), which is essential for converting pyruvate into acetyl-CoA, a key substrate for the citric acid cycle. The regulation of PDC by PDK2 determines whether cells utilize glucose or fatty acids for energy production, particularly under conditions of nutrient deprivation. PDK2 is predominantly expressed in heart and skeletal muscle tissues, where energy demands are high, and PDK2′s activity is modulated by various metabolic signals, including the availability of nutrients. PDK2′s structure features five conserved regions that are critical for kinase activity, and PDK2 shares mechanistic similarities with bacterial His-kinases, highlighting evolutionary significance. PDK2′s ability to phosphorylate the E1α subunit of PDC leads to the inactivation of the complex, thereby influencing metabolic pathways and energy homeostasis in response to physiological changes.

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PDK2 Antibody (180CT10.2.3) References:

1. Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2.  |  Kobayashi, T. and Cohen, P. 1999. Biochem J. 339 (Pt 2): 319-28. PMID: 10191262
2. Differential roles of PDK1- and PDK2-phosphorylation sites in the yeast AGC kinases Ypk1, Pkc1 and Sch9.  |  Roelants, FM., et al. 2004. Microbiology (Reading). 150: 3289-304. PMID: 15470109
3. The Novel Small Molecule Inhibitor, OSU-T315, Suppresses Vestibular Schwannoma and Meningioma Growth by Inhibiting PDK2 Function in the AKT Pathway Activation.  |  Mercado-Pimentel, ME., et al. 2016. Austin J Med Oncol. 3: PMID: 27642646
4. The Mitochondrial Rhomboid Protease PARL Is Regulated by PDK2 to Integrate Mitochondrial Quality Control and Metabolism.  |  Shi, G. and McQuibban, GA. 2017. Cell Rep. 18: 1458-1472. PMID: 28178523
5. MicroRNA-214 suppresses cell proliferation and migration and cell metabolism by targeting PDK2 and PHF6 in hepatocellular carcinoma.  |  Yu, Q., et al. 2020. Cell Biol Int. 44: 117-126. PMID: 31329335
6. Long noncoding RNA DUXAP8 contributes to the progression of hepatocellular carcinoma via regulating miR-422a/PDK2 axis.  |  Wei, F., et al. 2020. Cancer Med. 9: 2480-2490. PMID: 32022476
7. PDK2 leads to cisplatin resistance through suppression of mitochondrial function in ovarian clear cell carcinoma.  |  Kitamura, S., et al. 2021. Cancer Sci. 112: 4627-4640. PMID: 34464482
8. Chemokine (C-X-C motif) ligand 1 maintains the immune surveillance function of natural killer cells via the PDK2/mTOR signaling pathway.  |  Zeng, X., et al. 2023. Cell Biol Toxicol. 39: 2227-2241. PMID: 35304656
9. Extracellular vesicles derived from Lactobacillus plantarum restore chemosensitivity through the PDK2-mediated glucose metabolic pathway in 5-FU-resistant colorectal cancer cells.  |  An, J. and Ha, EM. 2022. J Microbiol. 60: 735-745. PMID: 35781627
10. Inhibition of pyruvate dehydrogenase kinase improves carbohydrate utilization in Nile tilapia by regulating PDK2/4-PDHE1α axis and insulin sensitivity.  |  Luo, Y., et al. 2022. Anim Nutr. 11: 25-37. PMID: 36016966

The preferred PDK2 antibody is PDK2 Antibody (S-15): sc-100534.