PDE6 beta抗体 (B-8) 推荐用于 mouse, rat 和 human 来源的precursor and mature PDE6β WB, IP, IF 和 ELISA检测

PDE6β 抗体 (B-8): sc-377486

PDE6β抗体 (B-8) is rated 4.7 out of 5 by 3.
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说明书

    • 抗-PDE6 beta 抗体 B-8 是小鼠单克隆 IgG1 κ,PDE6β抗体, 在2篇文献中引用,规格为200 µg/ml
    • 特异性抗原位于human物种的PDE6β的C-terminus附近的氨基酸779-811之间
    • 抗-PDE6 beta 抗体 (B-8) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 precursor and mature PDE6β; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
    • 抗-PDE6 beta 抗体 (B-8) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取PDE6β (B-8): sc-377486免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 PDE6β Antibody (B-8) for WB 的首选应用二抗。这些试剂现在以套装形式提供PDE6β Antibody (B-8)。 (见下方订购信息).
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What application is the blocking peptide sc-377486 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from PDE6b I used this antibody on OCT embedded frozen retinal sections (mouse). Blocked for 1 hour with 10% goat serum buffer. Incubated with primary antibody overnight at the a concentration of 1:100. Incubated with secondary antibody (alexa fluor 488 anti-mouse) at a concentration of 1:1000.
Date published: 2018-03-21
Rated 4 out of 5 by from Western blot O anticorpo funciona bem para Western blot no entanto identifica algumas bandas inespecíficas nas condições experimentais testadas
Date published: 2017-11-16
Rated 5 out of 5 by from Good Western blot data of PDE6 expression in non Good Western blot data of PDE6 expression in non-transfected and human PDE6 transfected 293 whole cell lysates. -SCBT QC
Date published: 2013-12-13
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