Date published: 2025-9-7

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PCM1 抗体 (G-6): sc-398365

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说明书
  • PCM1 抗体 G-6 是小鼠单克隆 IgG1 κ,PCM1抗体, 在35篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-262位于human物种的PCM1的N-terminus
  • PCM1 抗体 (G-6) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 PCM1
  • 抗PCM1抗体(G-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PCM1 (G-6): sc-398365免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是PCM1 Antibody (G-6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PCM1 Antibody (G-6) 打包提供(请参阅下面的订购信息)。

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関連項目

PCM1抗体(G-6)是一种IgG1 κ小鼠单克隆PCM1抗体(也称为HPCM-1抗体、PCM-1抗体、PTC4抗体、中心粒周围物质1 PCM1抗体或RET/PCM-1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的PCM1蛋白。PCM1抗体(G-6)既可以是非偶联的抗PCM1抗体形式,也可以是多种偶联形式的抗PCM1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。中心粒周围物质是一种动态物质,其组成在细胞周期中可以振荡。PCM1(中心粒周围物质1)是一种中心体蛋白,表现出与中心体复合物明显的细胞周期依赖性关联。PCM1在G1、S和部分G2期间与中心体复合物紧密结合。然而,在G2晚期,当细胞准备进行有丝分裂时,PCM1从中心体解离,然后在有丝分裂期间均匀扩散在整个细胞中,之后在G1期子代细胞中重新与中心体结合。PCM1在8p22-p21.3染色体上的染色体定位是令人感兴趣的,因为这个区域在几种肿瘤中常被删除。在甲状腺肿瘤组织中,PCM1的表达急剧下降,其亚细胞定位也发生了变化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PCM1 抗体 (G-6) 参考文献:

  1. RET/PCM-1:甲状腺乳头状癌中的新型融合基因。  |  Corvi, R., et al. 2000. Oncogene. 19: 4236-42. PMID: 10980597
  2. 将 Nek2 激酶动态招募到中心体涉及微管, PCM-1 和定域蛋白酶体降解。  |  Hames, RS., et al. 2005. Mol Biol Cell. 16: 1711-24. PMID: 15659651
  3. t(8;9)(p22;p24)是慢性和急性白血病中的一种复发性异常,它将 PCM1 与 JAK2 融合在一起。  |  Reiter, A., et al. 2005. Cancer Res. 65: 2662-7. PMID: 15805263
  4. 乳腺癌细胞系中染色体臂 8p 上候选肿瘤抑制基因位点的复杂 CGH 改变。  |  Venter, DJ., et al. 2005. Cancer Genet Cytogenet. 160: 134-40. PMID: 15993269
  5. JAK 触发器  |  Mahon, FX. 2005. Oncogene. 24: 7125-6. PMID: 16007127
  6. 骨髓增生性疾病和急性红细胞白血病中的 PCM1-JAK2 融合与 t(8;9) 易位。  |  Murati, A., et al. 2005. Leukemia. 19: 1692-6. PMID: 16034466
  7. 非典型慢性髓性白血病中的 t(8;9)(p22;p24)易位产生了一个新的 PCM1-JAK2 融合基因。  |  Bousquet, M., et al. 2005. Oncogene. 24: 7248-52. PMID: 16091753
  8. PCM1-JAK2融合:骨髓增生异常-骨髓增生异常和其他血液淋巴肿瘤的潜在治疗靶点。  |  Hoeller, S., et al. 2011. Expert Opin Ther Targets. 15: 53-62. PMID: 21091042
  9. PCM1标记揭示了不同物种骨骼肌中的肌核和核动态。  |  Viggars, MR., et al. 2023. Am J Physiol Cell Physiol. 324: C85-C97. PMID: 36409178
  10. 导航中心粒卫星:PCM1在细胞和生物体过程中的作用。  |  Begar, E., et al. 2024. FEBS J.. PMID: 38825736
  11. 人类自身抗原包膜材料 1 (PCM1) 基因在染色体 8p21.3-p22 上的映射。  |  Ohata, H., et al. 1994. Genomics. 24: 404-6. PMID: 7698772
  12. PCM-1,一种 228 kD 的中心体自身抗原,具有独特的细胞周期分布。  |  Balczon, R., et al. 1994. J Cell Biol. 124: 783-93. PMID: 8120099

订购信息

产品名称产品编号规格价格数量收藏夹

PCM1 抗体 (G-6)

sc-398365
200 µg/ml
$316.00

PCM1 (G-6): m-IgG Fc BP-HRP 套装

sc-530774
200 µg Ab; 10 µg BP
$354.00

PCM1 (G-6): m-IgGκ BP-HRP 套装

sc-524311
200 µg Ab, 40 µg BP
$354.00

PCM1 (G-6): m-IgG1 BP-HRP 套装

sc-544246
200 µg Ab; 20 µg BP
$354.00

PCM1 抗体 (G-6) AC

sc-398365 AC
500 µg/ml, 25% agarose
$416.00

PCM1 抗体 (G-6) HRP

sc-398365 HRP
200 µg/ml
$316.00

PCM1 抗体 (G-6) FITC

sc-398365 FITC
200 µg/ml
$330.00

PCM1 抗体 (G-6) PE

sc-398365 PE
200 µg/ml
$343.00

PCM1 抗体 (G-6) Alexa Fluor® 488

sc-398365 AF488
200 µg/ml
$357.00

PCM1 抗体 (G-6) Alexa Fluor® 546

sc-398365 AF546
200 µg/ml
$357.00

PCM1 抗体 (G-6) Alexa Fluor® 594

sc-398365 AF594
200 µg/ml
$357.00

PCM1 抗体 (G-6) Alexa Fluor® 647

sc-398365 AF647
200 µg/ml
$357.00

PCM1 抗体 (G-6) Alexa Fluor® 680

sc-398365 AF680
200 µg/ml
$357.00

PCM1 抗体 (G-6) Alexa Fluor® 790

sc-398365 AF790
200 µg/ml
$357.00

Hello. We have two aliquotes of PCM1 antibody (sc-398365). We want to know which Lot of them is the newest. We have Lot:B0119 and Lot:I1117 and we have different signals with them. Thaks for answer. L.Cupak

Asked by: Ladislav Cupak
Thank you for your question. Lot number B0119 is the newest. Please contact our Technical Service Department if you would like help in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-03-07

I just bough your antibody. I used it for IF staining of mouse heart sections but I have very high background. What should I do?

Asked by: Cardiology
We are sad to hear that this antibody did not perform for you as expected. We want you to be successful in your research, and we are happy to help you troubleshoot the antibody. Please contact our European Technical Service Department, you can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com. We are happy to help you troubleshoot the antibody!
Answered by: Tech Service
Date published: 2017-03-08

I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-398365: PCM1 (G-6) mouse monoclonal antibody will work?

Asked by: AbPolly
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 1 out of 5 by from It does not workI bought this as a free sample and it is not suitable for western
Date published: 2022-09-02
Rated 1 out of 5 by from No bands at the expected MWDetects a band (likely unspecific) at a much lower MW compared to the expected one (∼ 230 kDa) on lysates of Jurkat T cells.
Date published: 2019-01-22
Rated 5 out of 5 by from Works very well in ImmunofluorescenceWork very well in IF, give a specific signal with almost no background.
Date published: 2018-03-27
Rated 4 out of 5 by from Works great for IFIt works for IF both in human (HeLa) and mouse (A20). PFA fixation, 1:100
Date published: 2018-03-08
Rated 5 out of 5 by from This antibody works very nice!I tried this antibody on RPE1 wield type and RPE1 PCM1 knockout cell lines by IF(1:300), as a result this antibody works super nice! I am planning to do the western blot this week, I believe this antibody also will work super nice! Good antibody! I strongly recommend this antibody to every research who is interested in PCM1!
Date published: 2017-12-12
Rated 4 out of 5 by from good for IFThis antibody worked for IF in U2OS cells fixed in methanol @ 1:10.
Date published: 2017-11-14
Rated 4 out of 5 by from Good for ICCIts works good for ICC but we need more sample to try for WB
Date published: 2017-09-19
Rated 5 out of 5 by from Very good Western blot data of PCM1 expressionVery good Western blot data of PCM1 expression in K-562 and Ramos whole cell lysates. -SCBT QC
Date published: 2013-05-28
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PCM1 抗体 (G-6) is rated 3.6 out of 5 by 8.
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