Date published: 2025-9-11

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paxillin 抗体 (D-9): sc-365174

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说明书
  • paxillin抗体(D-9)是小鼠单克隆IgG1 κ, 在17篇文献中引用,规格为200 µg/ml
  • 针对human起源的paxillin内部区域内的氨基酸155-268进行
  • 抗-paxillin 抗体 (D-9) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 α,β, γ isoforms of paxillin
  • 抗paxillin抗体(D-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取paxillin (D-9): sc-365174免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是paxillin Antibody (D-9) for WB applications. 的首选辅助检测试剂,这些试剂现与paxillin Antibody (D-9) 打包提供(请参阅下面的订购信息)。

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関連項目

paxillin抗体(D-9)是一种IgG1 κ小鼠单克隆paxillin抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的paxillin的α、β、γ异构体。paxillin抗体(D-9)可作为非偶联形式的抗paxillin抗体,也可作为多种偶联形式的抗paxillin抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Paxillin是一种定位于细胞骨架的局灶性粘附磷酸蛋白。已证明,paxillin的磷酸化是对PDGF处理、v-src转化或整合素交联的响应。已证明,FAK(局灶性粘附激酶)和PYK2可使paxillin磷酸化。FAK在体外特异性地使paxillin的Tyr 118磷酸化。然而,FAK的磷酸化似乎对于paxillin招募到细胞粘附位点并不是必需的。Paxillin可能在信号转导、细胞形态调节以及结构和信号分子招募到局灶性粘附中发挥作用。已证明,在有丝分裂细胞中,通过蛋白水解下调,paxillin的数量会减少,并且在有丝分裂期间,paxillin会在丝氨酸上而不是在酪氨酸和丝氨酸上交替发生磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

paxillin 抗体 (D-9)

sc-365174
200 µg/ml
$316.00

paxillin (D-9): m-IgG Fc BP-HRP 套装

sc-527338
200 µg Ab; 10 µg BP
$354.00

paxillin (D-9): m-IgG1 BP-HRP 套装

sc-532711
200 µg Ab; 20 µg BP
$354.00

paxillin 抗体 (D-9) AC

sc-365174 AC
500 µg/ml, 25% agarose
$416.00

paxillin 抗体 (D-9) HRP

sc-365174 HRP
200 µg/ml
$316.00

paxillin 抗体 (D-9) FITC

sc-365174 FITC
200 µg/ml
$330.00

paxillin 抗体 (D-9) PE

sc-365174 PE
200 µg/ml
$343.00

paxillin 抗体 (D-9) Alexa Fluor® 488

sc-365174 AF488
200 µg/ml
$357.00

paxillin 抗体 (D-9) Alexa Fluor® 546

sc-365174 AF546
200 µg/ml
$357.00

paxillin 抗体 (D-9) Alexa Fluor® 594

sc-365174 AF594
200 µg/ml
$357.00

paxillin 抗体 (D-9) Alexa Fluor® 647

sc-365174 AF647
200 µg/ml
$357.00

paxillin 抗体 (D-9) Alexa Fluor® 680

sc-365174 AF680
200 µg/ml
$357.00

paxillin 抗体 (D-9) Alexa Fluor® 790

sc-365174 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with paxillin (D-9): sc-365174 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great for IFWorks great in immunofluorescence assay on human fibroblasts
Date published: 2019-05-14
Rated 5 out of 5 by from Great antibody for Western blotDetect a single band from hASMC extract on Western blot.
Date published: 2017-10-30
Rated 4 out of 5 by from Publishable data in WB using mouse BMDMsPublishable data in WB using mouse BMDMs -SCBT Publication Review
Date published: 2015-03-06
Rated 4 out of 5 by from Publishable data in IF using human A375 cellsPublishable data in IF using human A375 cells. -SCBT Publication Review
Date published: 2015-02-22
Rated 5 out of 5 by from Beautiful immunofluorescence focal adhesionsBeautiful immunofluorescence focal adhesions and membrane staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-03
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paxillin 抗体 (D-9) is rated 4.6 out of 5 by 5.
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