Date published: 2025-9-9

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paxillin 抗体 (B-2): sc-365379

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说明书
  • paxillin 抗体 B-2 是小鼠单克隆 IgG2b κ,paxillin抗体, 在31篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的paxillin的N-terminus附近的氨基酸17-47之间
  • paxillin 抗体 (B-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 paxillin isoforms α, β and γ; 也和以下物种反应,包括: 和 equine and canine
  • 抗paxillin抗体(B-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取paxillin (B-2): sc-365379免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是paxillin Antibody (B-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与paxillin Antibody (B-2) 打包提供(请参阅下面的订购信息)。

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関連項目

paxillin抗体(B-2)是一种IgG2b κ小鼠单克隆paxillin抗体(也称为PXN抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的paxillin蛋白。paxillin抗体(B-2)既可作为非偶联形式的抗paxillin抗体,也可作为多种偶联形式的抗paxillin抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Paxillin是一种定位于细胞骨架的局灶性粘附磷酸蛋白。已证明,paxillin的磷酸化是对PDGF处理、v-src转化或整合素交联的响应。已证明,FAK(局灶性粘附激酶)和PYK2可使paxillin磷酸化。FAK在体外特异性地使paxillin的Tyr 118磷酸化。然而,FAK的磷酸化似乎对于paxillin招募到细胞粘附位点并不是必需的。Paxillin可能在信号转导、细胞形态调节以及结构和信号分子招募到局灶性粘附中发挥作用。已证明,在通过蛋白水解下调的有丝分裂细胞中,paxillin的数量减少,在有丝分裂期间,paxillin在丝氨酸上发生磷酸化,而不是在酪氨酸和丝氨酸上交替发生磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

paxillin 抗体 (B-2) 参考文献:

  1. 补体受体 3(CR3,Mac-1,整合素 alpha M beta 2,CD11b/CD18)是粘附和非粘附中性粒细胞中 paxillin 酪氨酸磷酸化所必需的。  |  Graham, IL., et al. 1994. J Cell Biol. 127: 1139-47. PMID: 7525604
  2. 人 paxillin(一种被 P210BCR/ABL 磷酸化的病灶粘附蛋白)的分子克隆。  |  Salgia, R., et al. 1995. J Biol Chem. 270: 5039-47. PMID: 7534286
  3. 焦点粘附激酶在体外对 paxillin 进行酪氨酸磷酸化的特征。  |  Bellis, SL., et al. 1995. J Biol Chem. 270: 17437-41. PMID: 7615549
  4. 神经元体外分化过程中 paxillin 的酪氨酸磷酸化和表达增强。  |  Leventhal, PS. and Feldman, EL. 1996. J Biol Chem. 271: 5957-60. PMID: 8626373
  5. 鉴定 LIM3 是 paxillin 局灶粘附定位的主要决定因素,并鉴定 paxillin 上指导 vinculin 和局灶粘附激酶结合的新基序。  |  Brown, MC., et al. 1996. J Cell Biol. 135: 1109-23. PMID: 8922390
  6. 在大鼠肝上皮细胞中,Paxillin 被钙依赖性酪氨酸激酶磷酸化并优先与之结合。  |  Li, X. and Earp, HS. 1997. J Biol Chem. 272: 14341-8. PMID: 9162070
  7. 有丝分裂特异性丝氨酸磷酸化和下调病灶粘附蛋白之一 paxillin。  |  Yamaguchi, R., et al. 1997. Oncogene. 15: 1753-61. PMID: 9362441

订购信息

产品名称产品编号规格价格数量收藏夹

paxillin 抗体 (B-2)

sc-365379
200 µg/ml
$316.00

paxillin (B-2): m-IgG Fc BP-HRP 套装

sc-529365
200 µg Ab; 10 µg BP
$354.00

paxillin (B-2): m-IgGκ BP-HRP 套装

sc-522229
200 µg Ab, 40 µg BP
$354.00

paxillin (B-2): m-IgG2b BP-HRP 套装

sc-550099
200 µg Ab; 10 µg BP
$354.00

paxillin 抗体 (B-2) AC

sc-365379 AC
500 µg/ml, 25% agarose
$416.00

paxillin 抗体 (B-2) HRP

sc-365379 HRP
200 µg/ml
$316.00

paxillin 抗体 (B-2) FITC

sc-365379 FITC
200 µg/ml
$330.00

paxillin 抗体 (B-2) PE

sc-365379 PE
200 µg/ml
$343.00

paxillin 抗体 (B-2) Alexa Fluor® 488

sc-365379 AF488
200 µg/ml
$357.00

paxillin 抗体 (B-2) Alexa Fluor® 546

sc-365379 AF546
200 µg/ml
$357.00

paxillin 抗体 (B-2) Alexa Fluor® 594

sc-365379 AF594
200 µg/ml
$357.00

paxillin 抗体 (B-2) Alexa Fluor® 647

sc-365379 AF647
200 µg/ml
$357.00

paxillin 抗体 (B-2) Alexa Fluor® 680

sc-365379 AF680
200 µg/ml
$357.00

paxillin 抗体 (B-2) Alexa Fluor® 790

sc-365379 AF790
200 µg/ml
$357.00

paxillin (B-2) 中和勝肽

sc-365379 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-365379 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very good for Western blot and immunofluorescenceThis product works well in our WB and IF, we can see clear bands in WB, and beautiful staining of Paxillin at focal adhesions in IF!
Date published: 2023-11-22
Rated 3 out of 5 by from Immunofluorescence staining of podocyte cell lineWeak singals were detected in cytosol of fixed mouse podocyte cell line.
Date published: 2023-01-28
Rated 5 out of 5 by from 免疫荧光标记黏着斑我一个月前购买此商品,进行了免疫荧光实验,结果非常好,黏着斑标记的很清晰,而且抗体不是很贵,很满意。
Date published: 2022-09-07
Rated 4 out of 5 by from Immunofluorescence in cultured podocytesShowing cytoplasmic staining in cultured podocytes, fixed with 4% PFA. Dilution range 1:100 - 1:250
Date published: 2022-08-22
Rated 5 out of 5 by from very clear and specific band in WBDluition 1:1000 cells: C2C12; GN11. Application: Western Blotting Very clear and specific band
Date published: 2018-10-05
Rated 4 out of 5 by from Good antibody for WBDetects 2 bands at approx. 60 and 70 kDa. Dilution 1/1000, blocked with 2%BSA
Date published: 2018-03-07
Rated 4 out of 5 by from Works well in IFThis antibody works well in IF. Cytoplasmic signal in PFA fixed human colon carcinoma cells. It is not very strong so I suggest dilution 1:50.
Date published: 2017-09-27
Rated 5 out of 5 by from Top choice paxillin antibody!We offer many different options for paxillin antibodies, but this (B-2) clone is a top choice, with consistently excellent results in our internal testing of the antibodies. Highly recommended.
Date published: 2017-01-31
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paxillin 抗体 (B-2) is rated 4.5 out of 5 by 14.
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