paxillin Antibody (D-8): sc-365059

paxillin Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that specifically targets an epitope mapping between amino acids 17-47 near the N-terminus of human paxillin. Paxillin, also known as PXN, is a focal adhesion adaptor protein localized to the cytoskeleton, playing a critical role in signal transduction pathways that regulate cell adhesion, migration, and proliferation. Paxillin functions by recruiting structural and signaling molecules to focal adhesions, contact points between cells and the extracellular matrix, thus influencing cell shape and motility. Paxillin interacts with various proteins such as focal adhesion kinase (FAK), Src family kinases, vinculin, and integrins, forming complexes essential for transmitting signals that control cytoskeletal dynamics. The phosphorylation of paxillin on specific tyrosine residues, notably Tyr118, by kinases like FAK and Src, modulates paxillin activity and interactions, impacting processes like wound healing and cancer metastasis. During mitosis, paxillin levels are reduced through proteolytic downregulation, and paxillin is alternatively phosphorylated on serine residues, highlighting paxillin′s role in cell cycle regulation. anti-paxillin antibody (D-8) reacts with the α, β, and γ isoforms of paxillin from mouse, rat, human, equine, and canine origins in applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Understanding paxillin′s function is important because alterations in paxillin expression or activity are associated with various diseases, including cancer, making paxillin monoclonal antibody (D-8) a valuable tool for researchers studying cell adhesion and signaling pathways.

The preferred paxillin antibody is paxillin Antibody (B-2): sc-365379.