Date published: 2025-9-6
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PARP1 Antibody (F-2) - Western Blotting - Image 377572
PARP-1 抗体 (F-2): sc-8007. Jurkat (A), NTERA-2 cl. D1 (B) 和 SJRH30 (C) 全细胞裂解液中 PARP-1 表达的 Western 印迹分析.
PARP-1 抗体 (F-2): sc-8007. Jurkat (A), NTERA-2 cl. D1 (B) 和 SJRH30 (C) 全细胞裂解液中 PARP-1 表达的 Western 印迹分析.

PARP1 抗体 (F-2): sc-8007

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说明书
  • PARP1抗体(F-2)是小鼠单克隆IgG2a κ, 在893篇文献中引用，规格为200 µg/ml
  • 免疫human来源PARP位于C-terminus 的764-1014氨基酸
  • PARP1 抗体 (F-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 full-length PARP-1 and the C-terminal cleavage product of PARP-1
  • 抗PARP1抗体（F-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取PARP1 (F-2): sc-8007的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 PARP1 抗体 (F-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PARP1 抗体 (F-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外PARP抗体，包括pADPr, PARP-1, PARP-10, PARP-14, PARP-2, PARP-3和PARP-4

PARP-1抗体（F-2）是一种小鼠单克隆IgG2a kappa轻链抗体，通过识别764-1014氨基酸来靶向人类PARP-1的C端区域。PARP-1 单克隆抗体（F-2）可有效检测全长 PARP-1 蛋白及其 C 端裂解产物，因此 PARP-1 (F-2) 抗体是多种应用的宝贵工具，包括蛋白质印迹、免疫沉淀、免疫荧光、石蜡包埋组织免疫组织化学和酶联免疫吸附测定。PARP-1（聚（ADP-核糖）聚合酶1）也称为ARTD1、Adprt和Parp1，是一种重要的核酶，在通过碱基切除修复途径检测和修复DNA单链断裂中起着核心作用。在感知到DNA损伤后，PARP-1会与受损DNA结合，并催化将ADP-核糖单元从NAD⁺转移到自身和其他目标蛋白上，这一过程被称为PAR化。这种修饰有助于染色质重塑和更多DNA修复机制的招募，从而保持基因组的完整性，防止可能导致癌症等疾病的突变。PARP-1的结构包括一个识别DNA损伤的DNA结合结构域、一个通过PAR化作用进行自我调节的自动修饰结构域和一个负责酶促活性的催化结构域，确保对DNA损伤做出迅速有效的反应。此外，PARP-1还会经历翻译后修饰，包括凋亡过程中由半胱天冬酶进行的自修饰和切割。PARP-1与参与DNA修复和染色质结构的多个关键蛋白相互作用，例如XRCC1、DNA连接酶III、DNA聚合酶β和组蛋白，这表明其在协调DNA损伤反应和维护基因组稳定性方面发挥着重要作用。研究人员利用PARP-1小鼠单克隆抗体（F-2）可以研究DNA修复、细胞凋亡和基因组完整性维持的复杂机制，这使得抗PARP-1抗体（F-2）在分子生物学和医学研究方面具有重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PARP1 抗体 (F-2) 参考文献:

  1. 多聚（ADP-核糖）聚合酶在控制端粒长度和染色体稳定性方面的功能  |  d'Adda di Fagagna, F., et al. 1999. Nat Genet. 23: 76-80. PMID: 10471503
  2. 针对 DNA 损伤，通过聚（ADP-核糖基）合成调节转录的细胞防御途径。  |  Vispe, S., et al. 2000. Proc Natl Acad Sci U S A. 97: 9886-91. PMID: 10944198
  3. DNA 切割修复和 DNA 损伤诱导的细胞凋亡与聚（ADP-核糖基）生成有关，但对 p53 的要求不同。  |  Beneke, R., et al. 2000. Mol Cell Biol. 20: 6695-703. PMID: 10958667
  4. PARP 的世界。  |  Smith, S. 2001. Trends Biochem Sci. 26: 174-9. PMID: 11246023
  5. 多聚（ADP-核糖）聚合酶（PARP）在 DNA 修复, 基因组完整性和细胞死亡中的功能。  |  Herceg, Z. and Wang, ZQ. 2001. Mutat Res. 477: 97-110. PMID: 11376691
  6. 聚（ADP-核糖）糖水解酶在控制聚（ADP-核糖）代谢中的重要性。  |  Davidovic, L., et al. 2001. Exp Cell Res. 268: 7-13. PMID: 11461113
  7. PARP1的聚（ADP-核糖基化）：反应机制和调节蛋白。  |  Alemasova, EE. and Lavrik, OI. 2019. Nucleic Acids Res. 47: 3811-3827. PMID: 30799503
  8. 细胞毒性 T 细胞衍生颗粒酶 B 激活凋亡蛋白酶 CPP32  |  Darmon, AJ., et al. 1995. Nature. 377: 446-8. PMID: 7566124
  9. 具有类似 ICE 特性的蛋白酶对聚（ADP-核糖）聚合酶的裂解。  |  Lazebnik, YA., et al. 1994. Nature. 371: 346-7. PMID: 8090205
  10. 聚（ADP-核糖）聚合酶的特异性蛋白水解：化疗诱导细胞凋亡的早期标志。  |  Kaufmann, SH., et al. 1993. Cancer Res. 53: 3976-85. PMID: 8358726
  11. PARP 对基因组的稳定性很重要，但在细胞凋亡中却不可或缺。  |  Wang, ZQ., et al. 1997. Genes Dev. 11: 2347-58. PMID: 9308963
  12. DNA 修复：PARP--另一个守护天使？  |  Jeggo, PA. 1998. Curr Biol. 8: R49-51. PMID: 9427640

订购信息

产品名称产品编号规格价格数量收藏夹

PARP1 抗体 (F-2)

sc-8007
200 µg/ml
$316.00

PARP1 (F-2): m-IgG Fc BP-HRP 套装

sc-525446
200 µg Ab; 10 µg BP
$354.00

PARP1 (F-2): m-IgG2a BP-HRP 套装

sc-546260
200 µg Ab; 10 µg BP
$354.00

PARP1 抗体 (F-2) AC

sc-8007 AC
500 µg/ml, 25% agarose
$416.00

PARP1 抗体 (F-2) HRP

sc-8007 HRP
200 µg/ml
$316.00

PARP1 抗体 (F-2) FITC

sc-8007 FITC
200 µg/ml
$330.00

PARP1 抗体 (F-2) PE

sc-8007 PE
200 µg/ml
$343.00

PARP1 抗体 (F-2) Alexa Fluor® 488

sc-8007 AF488
200 µg/ml
$357.00

PARP1 抗体 (F-2) Alexa Fluor® 546

sc-8007 AF546
200 µg/ml
$357.00

PARP1 抗体 (F-2) Alexa Fluor® 594

sc-8007 AF594
200 µg/ml
$357.00

PARP1 抗体 (F-2) Alexa Fluor® 647

sc-8007 AF647
200 µg/ml
$357.00

PARP1 抗体 (F-2) Alexa Fluor® 680

sc-8007 AF680
200 µg/ml
$357.00

PARP1 抗体 (F-2) Alexa Fluor® 790

sc-8007 AF790
200 µg/ml
$357.00

PARP1 抗体 (F-2) TRITC

sc-8007 TRITC
200 µg/ml
$320.00

PARP1 抗体 (F-2) Alexa Fluor® 405

sc-8007 AF405
200 µg/ml
$357.00

I have to run western blot to detect PARP 1 and cleaved PARP in response to PARP 1 inhibition. Product PARP-1 (F-2), Catalog No: sc-8007 will serve the purpose or not

Asked by: Shazoo
Thank you for your question. For assistance please contact technical service. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-07-23

Can I use this antibody to detect mouse / rat PARP proteins?

Asked by: Ganapathi
Thank you for your question. western blot testing shows positive results in human and negative results in mouse and rat.
Answered by: BlakeJ
Date published: 2023-12-29

What is the cleaved parp-1 product that this antibody can detect such as 55kDa cleaved parp-1 or 89 kDa.

Asked by: Tirawit
Thank you for your question. PARP-1 Antibody (F-2) is recommended for detection of full-length PARP-1 and the C-terminal cleavage product of PARP-1. The C-terminal cleavage product has been described as 89 kDa.
Answered by: Technical Support
Date published: 2020-04-09

sc-8007 is good for FCM? any reference and/or protocol for this?

Asked by: Arielru
Thank you for your question. PARP-1 Antibody (F-2): sc-8007 is recommended for detection of full-length PARP-1 and the C-terminal cleavage product of PARP-1 of human origin by WB, IP, IF, IHC(P) and ELISA. It has not been tested conclusively for flow cytometry. We currently have no published data indicating how well this antibody is suited for flow cytometry. If you want to try this antibody for yourself please contact our technical service to arrange a free sample of the antibody.
Answered by: Tech Support Europe
Date published: 2019-07-01

Is the specificity of the antibody high enough to confirm a knockout of PARP1?

Asked by: Dr Maja
Thank you for your question. The sensitivity of a western blot is dependent on a lot of factors and may not be able to distinguish between a very efficient knockdown and a complete knockout of the protein of interested. You may want to perform western blotting alongside qRT PCR and Sanger sequencing for the most reliable results.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Will this detect the 24 kDa N-terminal cleavage product of PARP-1?

Asked by: Dr Ninau Qelp
PARP-1 Antibody (F-2): sc-8007 is specific to full-length PARP-1 and the C-terminal cleavage product of PARP-1. This antibody should NOT detect the 24 kDa N-terminal cleavage product of PARP-1.
Answered by: Tech Service 2
Date published: 2016-12-15

Can PARP-1 Antibody (F-2): sc-8007 be used for Flow Cytometry (FCM)?

Asked by: Dr Ninau Qelp
We have not tested this antibody for Flow Cytometry (FCM)?
Answered by: Tech Service 2
Date published: 2016-12-15

Hi, is this antibody can be detected by 'conventional secondary antibody', which is goat anti-mouse IgG-HRP? Because it seems some primary antibodies from your company which says just IgG (such as sc-11415, sc-5787) is detected but not this one.

Asked by: baizzang1
Thank you for your question. Our PARP-1 mouse monoclonal sc-8007 can be detected by conventional secondary antibodies such as goat anti-mouse IgG-HRP. We recommend using our mouse IgG binding protein, sc-516102. If you are having any difficulties getting good detection with this antibody, please contact your local Technical Service department.
Answered by: Technical Service
Date published: 2016-11-10
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Rated 5 out of 5 by from western blot PARP1We used this antibody to evaluate PARP1 expression by western blot in human cell lines. This antibody works very well with no background and extra bands
Date published: 2023-12-21
Rated 3 out of 5 by from Buon funzionamentoAbbiamo usato questo anticorpo su estratti totali di cellule SiHa. Si evidenzia una banda dell'altezza superiore di 116 kD nei campioni non trattati e dopo trattamenti specifici anche una banda di altezza minore (89 kD).
Date published: 2023-11-10
Rated 4 out of 5 by from very good for WBI could easily detect whole (130 kDa) and cleaved PARP (100 kDA) in HCT116 cells lysates
Date published: 2022-11-18
Rated 4 out of 5 by from 在PC12细胞上效果可以参考抗体的说没说进行对应的稀释，在目的分子量显示有明显的PARP1的条带，但是剪切的比较弱，没有背景！
Date published: 2022-07-26
Rated 5 out of 5 by from Works well.Specific blots in western blotting in cancer cell lines.
Date published: 2021-04-28
Rated 5 out of 5 by from goodThis antibody (PARP-1) worked well for western blot.
Date published: 2021-03-24
Rated 5 out of 5 by from Great antibody for wbI bought this antibody two weeks ago. This antibody is much better for wb compared with the previously discontinued one sc-7150. PS: This antibody is able to detect both full length and cleaved form of PARP1.
Date published: 2020-09-09
Rated 4 out of 5 by from PARP-1good antibody for Western blot, very clean for human cell extracts.
Date published: 2020-01-22
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PARP1 抗体 (F-2) is rated 4.5 out of 5 by 93.
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