Date published: 2025-9-7

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PARP1 抗体 (E-8): sc-74469

4.5(8)
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说明书
  • PARP1抗体(E-8)是小鼠单克隆IgG1 κ, 在24篇文献中引用,规格为200 µg/ml
  • 针对氨基酸1-300,在PARP-1的human起源的N-terminus处进行映射
  • 推荐用于 mouse, rat 和 human 来源的PARP-1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-74469 X, 200 µg/0.1 ml)
  • 看 PARP1 (F-2): sc-8007,可查看有标记的 PARP1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 PARP1 抗体 (E-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PARP1 抗体 (E-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

PARP-1抗体(E-8)是一种小鼠单克隆IgG1 kappa轻链抗体,针对人类PARP-1 N端1-300个氨基酸,可与小鼠、大鼠和人类样本反应,因此抗 -PARP-1抗体(E-8)适用于多种应用,如蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)。PARP-1,也称为多聚(ADP-核糖)聚合酶-1、ARTD1、Adprt和Parp1,是一种核DNA结合蛋白,在DNA修复、染色质重塑和细胞凋亡中起着关键作用。PARP-1主要位于细胞核中,对于识别DNA损伤(尤其是链断裂)以及通过催化ADP-核糖单元从NAD+转移到目标蛋白(包括组蛋白和其他与染色质相关的蛋白)来发出修复信号至关重要。PARP-1定位于细胞核,处于基因组维护的核心位置,能够快速响应DNA损伤,帮助防止可能导致染色体不稳定的突变。除了修复功能,PARP-1还影响转录和细胞死亡通路,因为在细胞凋亡过程中,PARP-1的裂解会产生一个89-kDa的片段,该片段会转移到细胞质中,从而促进细胞凋亡。PARP-1能够同时调节存活和死亡通路,这凸显了PARP-1在维持细胞稳态和基因组完整性方面的重要性。总之,PARP-1单克隆抗体(E-8)能够全面研究PARP-1在多种物种和实验平台中DNA修复、染色质重塑和细胞凋亡中的重要作用。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

PARP1 抗体 (E-8)

sc-74469
200 µg/ml
$316.00

PARP1 (E-8): m-IgG Fc BP-HRP 套装

sc-539524
200 µg Ab; 10 µg BP
$354.00

PARP1 (E-8): m-IgG1 BP-HRP 套装

sc-541544
200 µg Ab; 20 µg BP
$354.00

PARP1 抗体 (E-8) X

sc-74469 X
200 µg/0.1 ml
$316.00

When should the TransCruz reagent sc-74469 X be used instead of the standard form sc-74469?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good for WBWork fine in western blot analysis in A498 ccRCC cells
Date published: 2024-11-26
Rated 4 out of 5 by from Good for WBWork well for western blot analysis in rat renal tubular cells
Date published: 2023-08-18
Rated 5 out of 5 by from Publication in WBPublication in WB, EMSA and IF with paraformaldehyde fixed HepG2 samples.
Date published: 2015-04-17
Rated 3 out of 5 by from Okay results in WBOkay results in WB
Date published: 2015-03-31
Rated 5 out of 5 by from Great IF images obtained using formalinGreat IF images obtained using formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-25
Rated 5 out of 5 by from Great antibody for EMSAGreat antibody for EMSA, tested with rat UMR-106 cells. -SCBT Publication Review
Date published: 2015-02-09
Rated 5 out of 5 by from Produced superb immunofluorescence nuclearProduced superb immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-08
Rated 5 out of 5 by from Good Western blot data gathered in JurkatGood Western blot data gathered in Jurkat, HeLa and K-562 nuclear extracts. -SCBT QC
Date published: 2013-03-05
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PARP1抗体 (E-8) is rated 4.5 out of 5 by 8.
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