Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
p38 alpha/beta MAPK Antibody (A-12) - Western Blotting - Image 396284
p38α/β MAPK (A-12) Alexa Fluor® 680: sc-7972 AF680. HL-60 (A), K-562 (B), Jurkat (C), NIH/3T3 (D), RAW 264. 7 (E) 和 MCF7 (F) 全细胞裂解液中 p38α/β MAPK 表达的直接近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.
p38α/β MAPK (A-12) Alexa Fluor® 680: sc-7972 AF680. HL-60 (A), K-562 (B), Jurkat (C), NIH/3T3 (D), RAW 264. 7 (E) 和 MCF7 (F) 全细胞裂解液中 p38α/β MAPK 表达的直接近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.

p38 alpha/beta MAPK 抗体 (A-12): sc-7972

4.7(54)
寫評論提問

说明书
  • p38 alpha/beta MAPK 抗体 A-12 是小鼠单克隆 IgG1 κ，p38 alpha/beta MAPK抗体, 在484篇文献中引用，规格为200 µg/ml
  • 免疫human来源p38 alpha MAPK14位于C-terminus 的213-360氨基酸
  • p38 alpha/beta MAPK 抗体 (A-12) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 p38 alpha MAPK14 and p38β; 也和以下物种反应，包括： 和 equine and porcine
  • 抗p38 alpha/beta MAPK抗体（A-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取p38 alpha/beta MAPK (A-12): sc-7972的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 p38 alpha/beta MAPK 抗体 (A-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p38 alpha/beta MAPK 抗体 (A-12) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 p38 MAPK 抗体 包括 p38 alpha MAPK14, p38 beta MAPK11和p38 gamma MAPK12

p38α/β MAPK抗体（A-12）是一种小鼠单克隆IgG1κ轻链抗体，经过工程改造，可检测多种物种（包括小鼠、大鼠、人、马和猪）的p38α（MAPK14）和p38β（MAPK11）蛋白。p38α/β MAPK小鼠单克隆抗体（A-12）可识别 人类p38αMAPK14 C末端213-360氨基酸内的表位。p38 MAPKs，也称为SAPK2A/SAPK2B或p38a/p38b，是MAP激酶家族的重要成员，负责调节细胞应激反应、炎症、细胞因子产生和细胞凋亡等基本细胞过程。这些蛋白是信号转导通路中不可或缺的组成部分，可控制细胞分化、增殖和存活，从而维持细胞内环境稳定并响应环境刺激。p38α/β MAPK单克隆抗体（A-12）适用于包括蛋白质印迹（WB）、 免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA），使其成为研究和诊断的有用工具。通过靶向p38 MAPKs，p38 alpha/beta MAPK (A-12)抗体能够研究关键细胞机制以及癌症、自身免疫性疾病和炎症等疾病的潜在机制。p38 MAPKs依赖镁作为辅助因子，促进靶蛋白的ATP依赖性磷酸化，其活性通过 苏氨酸和酪氨酸残基，这对它们的功能至关重要。p38α/β MAPK单克隆抗体（A-12）有非结合型和多种结合型，包括琼脂糖、HRP、PE、FITC和几种Alexa Fluor®结合物，为研究人员提供了灵活的选择，以满足不同的实验需求。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p38 alpha/beta MAPK 抗体 (A-12) 参考文献:

  1. 红细胞生成素的表达需要 p38α：应激激酶在红细胞生成中的作用。  |  Tamura, K., et al. 2000. Cell. 102: 221-31. PMID: 10943842
  2. Exip 是 p38 alpha 的一种新的替代剪接变体，可诱导 HeLa 细胞更早地发生凋亡。  |  Sudo, T., et al. 2002. Biochem Biophys Res Commun. 291: 838-43. PMID: 11866441
  3. p38丝裂原活化蛋白激酶成员应激活化蛋白激酶-3（SAPK3）的心脏表达和亚细胞定域。  |  Court, NW., et al. 2002. J Mol Cell Cardiol. 34: 413-26. PMID: 11991731
  4. 人类 p38alpha 丝裂原活化蛋白激酶的活性突变体。  |  Diskin, R., et al. 2004. J Biol Chem. 279: 47040-9. PMID: 15284239
  5. p38和一种p38相互作用蛋白对小鼠胃形成过程中E-cadherin的下调至关重要。  |  Zohn, IE., et al. 2006. Cell. 125: 957-69. PMID: 16751104
  6. 人 p38 MAP 激酶的分子克隆。  |  Han, J., et al. 1995. Biochim Biophys Acta. 1265: 224-7. PMID: 7696354
  7. 一种参与调节炎症细胞因子生物合成的蛋白激酶。  |  Lee, JC., et al. Nature. 372: 739-46. PMID: 7997261
  8. 一种新的丝裂原活化蛋白激酶（p38beta）的结构和功能特征。  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
  9. p38 gamma 的主要结构：p38 MAP 激酶组的新成员。  |  Li, Z., et al. 1996. Biochem Biophys Res Commun. 228: 334-40. PMID: 8920915

订购信息

产品名称产品编号规格价格数量收藏夹

p38 alpha/beta MAPK 抗体 (A-12)

sc-7972
200 µg/ml
$316.00

p38 alpha/beta MAPK (A-12): m-IgG Fc BP-HRP 套装

sc-528193
200 µg Ab; 10 µg BP
$354.00

p38 alpha/beta MAPK (A-12): m-IgGκ BP-HRP 套装

sc-520515
200 µg Ab, 40 µg BP
$354.00

p38 alpha/beta MAPK (A-12): m-IgG1 BP-HRP 套装

sc-542808
200 µg Ab; 20 µg BP
$354.00

p38 alpha/beta MAPK 抗体 (A-12) AC

sc-7972 AC
500 µg/ml, 25% agarose
$416.00

p38 alpha/beta MAPK 抗体 (A-12) HRP

sc-7972 HRP
200 µg/ml
$316.00

p38 alpha/beta MAPK 抗体 (A-12) FITC

sc-7972 FITC
200 µg/ml
$330.00

p38 alpha/beta MAPK 抗体 (A-12) PE

sc-7972 PE
200 µg/ml
$343.00

p38 alpha/beta MAPK 抗体 (A-12) Alexa Fluor® 488

sc-7972 AF488
200 µg/ml
$357.00

p38 alpha/beta MAPK 抗体 (A-12) Alexa Fluor® 546

sc-7972 AF546
200 µg/ml
$357.00

p38 alpha/beta MAPK 抗体 (A-12) Alexa Fluor® 594

sc-7972 AF594
200 µg/ml
$357.00

p38 alpha/beta MAPK 抗体 (A-12) Alexa Fluor® 647

sc-7972 AF647
200 µg/ml
$357.00

p38 alpha/beta MAPK 抗体 (A-12) Alexa Fluor® 680

sc-7972 AF680
200 µg/ml
$357.00

p38 alpha/beta MAPK 抗体 (A-12) Alexa Fluor® 790

sc-7972 AF790
200 µg/ml
$357.00

p38 alpha/beta MAPK 抗体 (A-12) TRITC

sc-7972 TRITC
200 µg/ml
$320.00

p38 alpha/beta MAPK 抗体 (A-12) Alexa Fluor® 405

sc-7972 AF405
200 µg/ml
$357.00

hi, may i know if this antibody works in rat liver tissue?

Asked by: LlBb
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-05-10

Hi, I would like to know if this antibody has been tested (and if it is good) for IP? Thank you!

Asked by: stephanieT
Thank you for your question. p38α/β Antibody (A-12): sc-7972 has been tested and is recommended for detection of p38α and p38β of mouse, rat and human origin by IP, WB, IF, IHC(P), FCM and ELISA.
Answered by: Tech Support Europe
Date published: 2020-06-29

Hi, I am wondering if this antibody will be reactive with Oncorhynchus mykiss. Thank you!

Asked by: Yding
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support 10
Date published: 2017-11-28

Hi, I am curious to know if this antibody will work for salmonid p38 proteins (specifically Oncorhynchus mykiss). Would you be able to tell me what the epitope is and what % similarity the trout sequence must be to be cross-reactive with this antibody?

Asked by: Yding
Thank you for your question. We are not aware of any publications using this antibody, p38α/β (A-12): sc-7972, in trout and we have not tested this in house. The antibody is raised against amino acids 213-360 of p38α/β of human origin, however the exact epitope has not been epitope mapped so it would be fairly impossible to find out if it would work in trout samples. However, we do offer samples of this antibody, so if you would like to try it out on your samples, please contact our Technical Service Department by calling us toll-free at (800) 457-3801 ext. 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Tech Service
Date published: 2017-10-20

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-7972 PE), fluorescein (sc-7972 FITC), Alexa Fluor® 488 (sc-7972 AF488) or Alexa Fluor® 647 (sc-7972 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2017-01-04
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_zh_CN, sid_7972, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 112ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great for WBIt is a good antibody in WB. It works in dilution 1:1000.
Date published: 2024-03-19
Rated 5 out of 5 by from The antibody works wellFew months ago, we used this antibody to determine the level of p38 in mouse colon tissues by Western blot. The band was clear and precise.
Date published: 2024-01-02
Rated 5 out of 5 by from Good AntibodyMultiple bands were detected but still expected size band was detected.
Date published: 2021-12-03
Rated 5 out of 5 by from ExcellentI bought this antibody a few years ago and the result is good and clear.
Date published: 2021-11-01
Rated 5 out of 5 by from Excellent for western blotAntibody worked very well in rat thyroid FRTL5 cells for western blot. I used 1:1000 in TBS-BSA 5% 1h RT and got great signal.
Date published: 2021-02-23
Rated 5 out of 5 by from Good antibody for western blottingp38 Antibody (A-12): sc-7972 is good quality for western blotting (dilute factor: 1:1000)
Date published: 2020-05-25
Rated 5 out of 5 by from Excellent antibodyWestern blot: working dilution 1:2000 in blotto A, 1h of incubation, 25 ug of proteins loaded, clear band without background.
Date published: 2019-10-03
Rated 5 out of 5 by from ExcellentThis antibody works great for western blotting.Excellent
Date published: 2019-07-30
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_53
  • loc_zh_CN, sid_7972, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
p38 alpha/beta MAPK 抗体 (A-12) is rated 4.7 out of 5 by 54.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_53
  • loc_zh_CN, sid_7972, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 108ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。