Date published: 2025-9-6
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CDKN1B/Kip1 p27 Antibody (DCS-72) - Western Blotting - Image 17345
p27 Kip1 抗体 (DCS-72): sc-56338. Jurkat (A), HeLa (B), MCF7 (C), MM-142 (D) 和 NIH/3T3 (E) 全细胞裂解液中 p27 Kip1 表达的 Western 印迹分析.
p27 Kip1 抗体 (DCS-72): sc-56338. Jurkat (A), HeLa (B), MCF7 (C), MM-142 (D) 和 NIH/3T3 (E) 全细胞裂解液中 p27 Kip1 表达的 Western 印迹分析.

CDKN1B/Kip1 p27 抗体 (DCS-72): sc-56338

4.7(9)
寫評論提問

说明书
  • CDKN1B/Kip1 p27抗体(DCS-72)是小鼠单克隆IgG1 κ, 在31篇文献中引用，规格为200 µg/ml
  • 针对mouse来源的全长p27 Kip1
  • 抗-CDKN1B/Kip1 p27 抗体 (DCS-72) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 p27 Kip1; 也和以下物种反应，包括： canine; 不会与 other mitotic inhibitors产生交联反应
  • 抗CDKN1B/Kip1 p27抗体（DCS-72）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CDKN1B/Kip1 p27 (DCS-72): sc-56338的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CDKN1B/Kip1 p27 抗体 (DCS-72) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CDKN1B/Kip1 p27 抗体 (DCS-72) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p27 Kip1 抗体（DCS-72）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人样本中的 p27 Kip1 蛋白。p27 Kip1 抗体 (DCS-72) 有非共轭型和多种共轭型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭合物。p27 Kip1 主要在细胞核中发挥作用，与 D 型细胞周期蛋白和 Cdk4 相互作用，调节它们的活性。各种信号通路都会影响 p27 Kip1 的水平，包括由转化生长因子-β（TGFβ）激活的可引发 G1 停滞的通路，这证明了 p27 Kip1 在细胞应激和损伤反应中的作用。使用抗 p27 Kip1 抗体 (DCS-72) 可以帮助研究人员探索细胞周期调控机制及其在癌症生物学和治疗开发中的意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CDKN1B/Kip1 p27 抗体 (DCS-72)

sc-56338
200 µg/ml
$316.00

CDKN1B/Kip1 p27 (DCS-72): m-IgG Fc BP-HRP 套装

sc-526882
200 µg Ab; 10 µg BP
$354.00

CDKN1B/Kip1 p27 (DCS-72): m-IgG1 BP-HRP 套装

sc-532255
200 µg Ab; 20 µg BP
$354.00

CDKN1B/Kip1 p27 抗体 (DCS-72) AC

sc-56338 AC
500 µg/ml, 25% agarose
$416.00

CDKN1B/Kip1 p27 抗体 (DCS-72) HRP

sc-56338 HRP
200 µg/ml
$316.00

CDKN1B/Kip1 p27 抗体 (DCS-72) FITC

sc-56338 FITC
200 µg/ml
$330.00

CDKN1B/Kip1 p27 抗体 (DCS-72) PE

sc-56338 PE
200 µg/ml
$343.00

CDKN1B/Kip1 p27 抗体 (DCS-72) Alexa Fluor® 488

sc-56338 AF488
200 µg/ml
$357.00

CDKN1B/Kip1 p27 抗体 (DCS-72) Alexa Fluor® 546

sc-56338 AF546
200 µg/ml
$357.00

CDKN1B/Kip1 p27 抗体 (DCS-72) Alexa Fluor® 594

sc-56338 AF594
200 µg/ml
$357.00

CDKN1B/Kip1 p27 抗体 (DCS-72) Alexa Fluor® 647

sc-56338 AF647
200 µg/ml
$357.00

CDKN1B/Kip1 p27 抗体 (DCS-72) Alexa Fluor® 680

sc-56338 AF680
200 µg/ml
$357.00

CDKN1B/Kip1 p27 抗体 (DCS-72) Alexa Fluor® 790

sc-56338 AF790
200 µg/ml
$357.00

I am using p27 (DCS-72): sc-56338 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibody!1:200 diluted for wb of rat heart protein,the band is strong .
Date published: 2018-03-19
Rated 3 out of 5 by from p27 on zebrafishWe tried p27 DCS72 on zebrafish protein and we see a slight sign on WB, there are some lines if you stain it overnight at 4°C, at a concetration of 1:500, with a secunder ab concentration of 1:000. We would be happy to see more but it is better than if we stain it on RT, and at least it is principially not recommended for zebrafish. There are aspecific lines too.
Date published: 2017-06-14
Rated 5 out of 5 by from Very nice band for p27 detectionWe used this antibody for p27 expression detection with western blot. We got a very nice band at the correct molecular weight for HeLa cells
Date published: 2017-01-31
Rated 5 out of 5 by from p27 antibodyBought recently and works well to detect p27 at the correct kDa
Date published: 2016-12-22
Rated 4 out of 5 by from Good results using this antibody on humanGood results using this antibody on human RPMI 8226, NCI H929 and bone marrow stromal cell lines HS-5. -SCBT Publication Review
Date published: 2015-05-03
Rated 5 out of 5 by from Used to confirm presence and absence of p27Used to confirm presence and absence of p27 after using our p27 CRISPR/CAS9 KO Plasmid (m): sc-419608 with NIH/3T3 cells. -SCBT QC
Date published: 2015-01-25
Rated 5 out of 5 by from Published data obtained using DCSPublished data obtained using DCS-72 with CaLu-6 - anaplastic lung cancer cells. -SCBT Publication Review
Date published: 2015-01-13
Rated 5 out of 5 by from Good antibody for WB: cleanGood antibody for WB: clean, singular bands observed in Jurkat, HeLa, MCF7, MM-142 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-01-11
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CDKN1B/Kip1 p27 抗体 (DCS-72) is rated 4.7 out of 5 by 9.
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