Date published: 2025-9-6
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CDKN1B/Kip1 p27 Antibody (F-8) - Western Blotting - Image 372750
p27 Kip1 抗体 (F-8): sc-1641. MM-142 (A), RAW 264. 7 (B), NAMALWA (C), BJAB (D), Raji (E) 和 C6 (F) 全细胞裂解液中 p27 Kip1 表达的 Western 印迹分析.
p27 Kip1 抗体 (F-8): sc-1641. MM-142 (A), RAW 264. 7 (B), NAMALWA (C), BJAB (D), Raji (E) 和 C6 (F) 全细胞裂解液中 p27 Kip1 表达的 Western 印迹分析.

CDKN1B/Kip1 p27 抗体 (F-8): sc-1641

4.8(49)
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说明书
  • CDKN1B/Kip1 p27 抗体 F-8 是小鼠单克隆 IgG1 κ，CDKN1B/Kip1 p27抗体, 在673篇文献中引用，规格为200 µg/ml
  • 免疫1-197氨基酸，对应于mouse来源p27 Kip1全长
  • CDKN1B/Kip1 p27 抗体 (F-8) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 p27 Kip1
  • 抗CDKN1B/Kip1 p27抗体（F-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取CDKN1B/Kip1 p27 (F-8): sc-1641的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CDKN1B/Kip1 p27 抗体 (F-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CDKN1B/Kip1 p27 抗体 (F-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

p27 Kip1抗体（F-8）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类的p27 Kip1，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）。p27 Kip1（F- 8）抗体有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合型。p27 Kip1作为G1期转换的负调控因子，在调节细胞周期进展中发挥着关键作用，而G1期转换对于维持细胞正常功能和防止细胞无序增殖至关重要。在DNA损伤时，特别是以p53依赖的方式，p27 Kip1的水平会升高，这凸显了其在细胞应激反应中的重要性。p27 Kip1与D型细胞周期蛋白和细胞周期蛋白依赖性激酶4相互作用， 以及较小程度上与细胞周期蛋白E和细胞周期蛋白依赖性激酶2相互作用，从而影响细胞周期中这些关键调节因子的活性。p27 Kip1对细胞周期动态的调节凸显了其在癌症生物学和针对细胞增殖的治疗策略中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CDKN1B/Kip1 p27 抗体 (F-8) 参考文献:

  1. p27(Kip1)信号：转录和翻译后调节。  |  Hnit, SS., et al. 2015. Int J Biochem Cell Biol. 68: 9-14. PMID: 26279144
  2. p27kip1作为子宫内膜异位症的关键调节因子。  |  Gonçalves, GA. 2018. Eur J Obstet Gynecol Reprod Biol. 221: 1-4. PMID: 29216564
  3. p27Kip1 调节α-突触核蛋白的表达。  |  Gallastegui, E., et al. 2018. Oncotarget. 9: 16368-16379. PMID: 29662651
  4. 克隆细胞周期蛋白依赖性激酶抑制因子 p27Kip1，它是细胞外抗抑制信号的潜在媒介。  |  Polyak, K., et al. 1994. Cell. 78: 59-66. PMID: 8033212
  5. p27 是一种新型的 G1 细胞周期蛋白-Cdk 蛋白激酶活性抑制剂，与 p21 有关。  |  Toyoshima, H. and Hunter, T. 1994. Cell. 78: 67-74. PMID: 8033213
  6. p15INK4B 是 TGF-beta 诱导的细胞周期停滞的潜在效应因子。  |  Hannon, GJ. and Beach, D. 1994. Nature. 371: 257-61. PMID: 8078588
  7. 一种受细胞周期调控的细胞周期蛋白依赖性激酶抑制剂。  |  Hengst, L., et al. 1994. Proc Natl Acad Sci U S A. 91: 5291-5. PMID: 8202483
  8. WAF1，p53 抑制肿瘤的潜在媒介。  |  el-Deiry, WS., et al. 1993. Cell. 75: 817-25. PMID: 8242752
  9. p21 是一种通用的细胞周期蛋白激酶抑制剂。  |  Xiong, Y., et al. 1993. Nature. 366: 701-4. PMID: 8259214
  10. 细胞周期控制中的一种新的调节基因，可对细胞周期蛋白 D/CDK4 产生特异性抑制作用。  |  Serrano, M., et al. 1993. Nature. 366: 704-7. PMID: 8259215
  11. p27Kip1是一种细胞周期蛋白-Cdk抑制剂，它将转化生长因子-β和接触抑制与细胞周期停滞联系在一起。  |  Polyak, K., et al. 1994. Genes Dev. 8: 9-22. PMID: 8288131
  12. 哺乳动物 G1 细胞周期蛋白  |  Sherr, CJ. 1993. Cell. 73: 1059-65. PMID: 8513492

订购信息

产品名称产品编号规格价格数量收藏夹

CDKN1B/Kip1 p27 抗体 (F-8)

sc-1641
200 µg/ml
$316.00

CDKN1B/Kip1 p27 (F-8): m-IgG Fc BP-HRP 套装

sc-526469
200 µg Ab; 10 µg BP
$354.00

CDKN1B/Kip1 p27 (F-8): m-IgG1 BP-HRP 套装

sc-531842
200 µg Ab; 20 µg BP
$354.00

CDKN1B/Kip1 p27 抗体 (F-8) AC

sc-1641 AC
500 µg/ml, 25% agarose
$416.00

CDKN1B/Kip1 p27 抗体 (F-8) HRP

sc-1641 HRP
200 µg/ml
$316.00

CDKN1B/Kip1 p27 抗体 (F-8) FITC

sc-1641 FITC
200 µg/ml
$330.00

CDKN1B/Kip1 p27 抗体 (F-8) PE

sc-1641 PE
200 µg/ml
$343.00

CDKN1B/Kip1 p27 抗体 (F-8) Alexa Fluor® 488

sc-1641 AF488
200 µg/ml
$357.00

CDKN1B/Kip1 p27 抗体 (F-8) Alexa Fluor® 546

sc-1641 AF546
200 µg/ml
$357.00

CDKN1B/Kip1 p27 抗体 (F-8) Alexa Fluor® 594

sc-1641 AF594
200 µg/ml
$357.00

CDKN1B/Kip1 p27 抗体 (F-8) Alexa Fluor® 647

sc-1641 AF647
200 µg/ml
$357.00

CDKN1B/Kip1 p27 抗体 (F-8) Alexa Fluor® 680

sc-1641 AF680
200 µg/ml
$357.00

CDKN1B/Kip1 p27 抗体 (F-8) Alexa Fluor® 790

sc-1641 AF790
200 µg/ml
$357.00

CDKN1B/Kip1 p27 抗体 (F-8) TRITC

sc-1641 TRITC
200 µg/ml
$320.00

CDKN1B/Kip1 p27 抗体 (F-8) Alexa Fluor® 405

sc-1641 AF405
200 µg/ml
$357.00

Do you get this product and dilute it or do you use it as is, and if you dilute it, what is the dilution ratio?

Asked by: wjdghks4079
Thank you for your question. https://datasheets.scbt.com/sc-1641.pdf APPLICATIONS p27 Kip1 (F-8) is recommended for detection of p27 Kip1 of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100 1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50 1:500), immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500), flow cytometry (1 µg per 1 x 106 cells) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
Answered by: Ling Chen
Date published: 2025-08-11

Good morning Excuse me, I'm interested in buying the p27 antibody, but I would like to know if there is a way to obtain a sample to determine the affinity for the technique and then buy it.

Asked by: Alan Hipólito Juárez Sola
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Service
Date published: 2023-03-01

Does this antibody work for frozen tissue sections? If not, could I get a recommendation?

Asked by: Noa R
Thank you for your question. p27 Antibody (F-8): sc-1641 is recommended for detection of p27 in both frozen and paraffin-embedded tissue sections.
Answered by: Tech Support Europe
Date published: 2020-08-28

What is the expected localization of the p27 antigen for detection in tissue?

Asked by: TinTin
Thank you for your question. The p27 protein has cytoplasmic and nuclear expression in most tissues. This is consistent with the IHC(P) data on our website and datasheet for this antibody. And also supported by the Human Protein Atlas (see reference below for details). http://www.proteinatlas.org/ENSG00000111276-CDKN1B/tissue
Answered by: Technical Support
Date published: 2016-12-29
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Rated 4 out of 5 by from Good for WBWork fine in western blot analysis in A498 ccRCC cells
Date published: 2024-11-26
Rated 5 out of 5 by from sc-1641We use it to detect p27 protein expression in western blot, and it works well.
Date published: 2023-07-07
Rated 5 out of 5 by from Great results on canine melanocytic tumors (IHC)Tested 1:100 with HIER (pH 9.0; Tris-EDTA). Nice and specific staining.
Date published: 2022-12-06
Rated 5 out of 5 by from Great antibody for WBThis primary antibody worked very well in Western blot.
Date published: 2021-03-31
Rated 5 out of 5 by from This Antibody works in WBThis Antibody (E-5) clearly detect p27kip1 through western bloting method.
Date published: 2020-12-01
Rated 4 out of 5 by from はっきりしたバンドウエスタンブロットで使用しました。薬剤による発現誘導がしっかり確認できました。ノンスペもなくはっきりした画像が得られました。
Date published: 2020-11-13
Rated 5 out of 5 by from WBで使用可能RPE1細胞のWBで使用し、いい結果が得られました。1:500希釈で27kDa付近にはっきりとしたバンドが検出されました。
Date published: 2020-08-06
Rated 5 out of 5 by from Verified ABThis AB was verified via immuno fluorescence in pCMV5-human P27 transfected HEK293T cells. Negative control was non transfected HRK293T which has p27 staining equal to that of autofluorescence background. Serial dilutions of AB 1:50-1:500 showed only marginal decrease in staining efficiency. This is an excellent AB, that stains bright and is ideally colocalized with the DAPI nuclear stain.
Date published: 2019-03-01
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CDKN1B/Kip1 p27 抗体 (F-8) is rated 4.8 out of 5 by 49.
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