p-tuberin Antibody (325.Ser 1798): sc-293149

p-tuberin Antibody (325.Ser 1798) is a mouse monoclonal IgG1 kappa light chain antibody that detects phosphorylated tuberin at Ser 1798 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). p-tuberin (325.Ser 1798) antibody is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. Tuberin, also known as TSC2, plays a crucial role in cell growth and proliferation regulation by forming a functional complex with hamartin (TSC1), which inhibits protein synthesis and acts as a tumor suppressor. Tuberin′s function is vital in the tuberous sclerosis complex (TSC), a genetic disorder caused by mutations in TSC1 and TSC2 genes, leading to benign tumor development in multiple organs. Phosphorylation of tuberin at specific sites, such as serine-939 and threonine-1462, by the phosphoinositide 3-kinase (PI3K)/Akt pathway, inhibits the tumor suppressor function of the TSC complex, promoting cell growth. Phosphorylation of tuberin by the p38-MK2 kinase cascade at serine-1210 creates a binding site for 14-3-3 proteins, which regulates the hamartin-tuberin complex activity during the G2/M phase of the cell cycle. This regulation highlights p-tuberin′s significance in maintaining cellular homeostasis and potential implications in cancer biology and therapeutic interventions.

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p-tuberin Antibody (325.Ser 1798) References:

1. The p38 and MK2 kinase cascade phosphorylates tuberin, the tuberous sclerosis 2 gene product, and enhances its interaction with 14-3-3.  |  Li, Y., et al. 2003. J Biol Chem. 278: 13663-71. PMID: 12582162
2. Inactivation of the tuberous sclerosis complex-1 and -2 gene products occurs by phosphoinositide 3-kinase/Akt-dependent and -independent phosphorylation of tuberin.  |  Tee, AR., et al. 2003. J Biol Chem. 278: 37288-96. PMID: 12867426
3. Tuberous sclerosis complex gene products, Tuberin and Hamartin, control mTOR signaling by acting as a GTPase-activating protein complex toward Rheb.  |  Tee, AR., et al. 2003. Curr Biol. 13: 1259-68. PMID: 12906785
4. Cell cycle-regulated phosphorylation of hamartin, the product of the tuberous sclerosis complex 1 gene, by cyclin-dependent kinase 1/cyclin B.  |  Astrinidis, A., et al. 2003. J Biol Chem. 278: 51372-9. PMID: 14551205
5. Estrogen-induced smooth muscle cell growth is regulated by tuberin and associated with altered activation of platelet-derived growth factor receptor-beta and ERK-1/2.  |  Finlay, GA., et al. 2004. J Biol Chem. 279: 23114-22. PMID: 15039427
6. Regulation of B-Raf kinase activity by tuberin and Rheb is mammalian target of rapamycin (mTOR)-independent.  |  Karbowniczek, M., et al. 2004. J Biol Chem. 279: 29930-7. PMID: 15150271
7. Human papillomavirus 16 E6 oncoprotein interferences with insulin signaling pathway by binding to tuberin.  |  Lu, Z., et al. 2004. J Biol Chem. 279: 35664-70. PMID: 15175323
8. Tuberin binds p27 and negatively regulates its interaction with the SCF component Skp2.  |  Rosner, M. and Hengstschläger, M. 2004. J Biol Chem. 279: 48707-15. PMID: 15355997
9. Transgenic expression of dominant negative tuberin through a strong constitutive promoter results in a tissue-specific tuberous sclerosis phenotype in the skin and brain.  |  Govindarajan, B., et al. 2005. J Biol Chem. 280: 5870-4. PMID: 15576369
10. Tuberin nuclear localization can be regulated by phosphorylation of its carboxyl terminus.  |  York, B., et al. 2006. Mol Cancer Res. 4: 885-97. PMID: 17114346
11. Tuberin: a stimulus-regulated tumor suppressor protein controlled by a diverse array of receptor tyrosine kinases and G protein-coupled receptors.  |  Wu, EH., et al. Neurosignals. 15: 217-27. PMID: 17389815
12. The tuberous sclerosis gene products hamartin and tuberin are multifunctional proteins with a wide spectrum of interacting partners.  |  Rosner, M., et al. 2008. Mutat Res. 658: 234-46. PMID: 18291711

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-tuberin Antibody (325.Ser 1798) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-p-tuberin Antibody (325.Ser 1798) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-tuberin Antibody (325.Ser 1798) Specifications

Alternate Product Name: TSC2

Product Clone Name: 325.Ser 1798

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 1798

Epitope Target: tuberin

Epitope Species: human

Product Reactivity/Detection: Ser 1798 phosphorylated tuberin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for p-tuberin Antibody (325.Ser 1798): AC: Agarose, HRP: Horseradish Peroxidase