p-Rsk抗体 (C-5) 推荐用于 mouse, rat 和 human 来源的Ser 380 phosphorylated Rsk WB, IP, IF 和 ELISA检测

p-Rsk 抗体 (C-5): sc-377526

p-Rsk抗体 (C-5) is rated 4.5 out of 5 by 4.
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说明书

    • p-Rsk抗体(C-5)是小鼠单克隆IgG2a κ, 在2篇文献中引用,规格为200 µg/ml
    • specific for an epitope mapping to a short amino acid sequence containing phosphorylated Ser 380 of Rsk -1 of human origin
    • 抗-p-Rsk 抗体 (C-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Ser 380 phosphorylated Rsk
    • 抗-p-Rsk 抗体 (C-5) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取p-Rsk (C-5): sc-377526免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP 是 p-Rsk Antibody (C-5) for WB首选应用二抗。这些试剂现在以套装形式提供p-Rsk Antibody (C-5) (见下方订购信息).
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What application is the blocking peptide sc-377526 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Muy buen resultado para WB Muy buen resultado para WB. La banda es específica en extractos celulares totales de células AGS.
Date published: 2018-01-11
Rated 4 out of 5 by from Works well for WB works well for western - samples were human cells in culture
Date published: 2017-11-21
Rated 4 out of 5 by from Good antibody for Western blotting This monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and was determined to be suitable for Western blotting. It provided medium detection of the Rsk target in immunoblots of lysates from HeLa cells that were exposed to anisomycin for 2 hours, but no other cross-reactivities were observed. There was no detection of the Rsk target in overnight serum-starved human A431 cells treated for 5 minutes with epidermal growth factor on Western blots, but again this antibody was very clean and no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2016-12-08
Rated 5 out of 5 by from Nice Western blot data of Rsk phosphorylation Nice Western blot data of Rsk phosphorylation in untreated, PMA treated and PMA and lambda protein phosphatase treated HeLa whole cell lysates. -SCBT QC
Date published: 2013-11-03
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