p-Raf-1 Antibody (H-8): sc-271928
- p-Raf-1 Antibody (H-8) is a mouse monoclonal IgG2b κ, cited in 5 publications, provided at 200 µg/ml
- raised against a short amino acid sequence containing phosphorylated Ser 621 of Raf-1 of human origin
- recommended for detection of Ser 621 phosphorylated Raf-1 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine and avian
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-Raf-1 Antibody (H-8) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Raf-1 Antibody (H-8) (see ordering information below).
p-Raf-1 Antibody (H-8) is a mouse monoclonal IgG2b antibody that detects p-Raf-1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-Raf-1, also known as phosphorylated Raf-1, plays a crucial role in cell signaling pathways that regulate cell growth, differentiation, and survival. p-Raf-1 is primarily located in the cytoplasm, where p-Raf-1 acts as a key mediator in the Ras-Raf-MEK-ERK signaling cascade, which is vital for transmitting signals from growth factor receptors to the nucleus. This pathway is essential for normal cellular functions, and dysregulation can lead to oncogenic transformations and various cancers. The activation of p-Raf-1 is tightly controlled through phosphorylation at specific serine and tyrosine residues, which modulates kinase activity and interaction with downstream effectors. Understanding the precise localization and function of p-Raf-1 is important for elucidating p-Raf-1′s role in both normal physiology and disease states, making anti-p-Raf-1 antibody (H-8) an invaluable tool for researchers studying these critical processes.
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p-Raf-1 Antibody (H-8) References:
- Regulation of the Raf-1 kinase domain by phosphorylation and 14-3-3 association. | Yip-Schneider, MT., et al. 2000. Biochem J. 351: 151-9. PMID: 10998357
- S338 phosphorylation of Raf-1 is independent of phosphatidylinositol 3-kinase and Pak3. | Chiloeches, A., et al. 2001. Mol Cell Biol. 21: 2423-34. PMID: 11259591
- Erythropoietin-stimulated Raf-1 tyrosine phosphorylation is associated with the tyrosine kinase Lyn in J2E erythroleukemic cells. | Tilbrook, PA., et al. 2001. Arch Biochem Biophys. 396: 128-32. PMID: 11716471
- Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259. | Dhillon, AS., et al. 2002. Mol Cell Biol. 22: 3237-46. PMID: 11971957
- p21-activated Kinase 1 (Pak1)-dependent phosphorylation of Raf-1 regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association. | Jin, S., et al. 2005. J Biol Chem. 280: 24698-705. PMID: 15849194
- Identification of novel in vivo Raf-1 phosphorylation sites mediating positive feedback Raf-1 regulation by extracellular signal-regulated kinase. | Balan, V., et al. 2006. Mol Biol Cell. 17: 1141-53. PMID: 16407412
- Characterization of Ser338 phosphorylation for Raf-1 activation. | Zang, M., et al. 2008. J Biol Chem. 283: 31429-37. PMID: 18775988
- Mammalian Ste20-like kinase (Mst2) indirectly supports Raf-1/ERK pathway activity via maintenance of protein phosphatase-2A catalytic subunit levels and consequent suppression of inhibitory Raf-1 phosphorylation. | Kilili, GK. and Kyriakis, JM. 2010. J Biol Chem. 285: 15076-15087. PMID: 20212043
- Raf-1 forms a stable complex with Mek1 and activates Mek1 by serine phosphorylation. | Huang, W., et al. 1993. Proc Natl Acad Sci U S A. 90: 10947-51. PMID: 8248196
- Protein kinase C alpha activates RAF-1 by direct phosphorylation. | Kolch, W., et al. 1993. Nature. 364: 249-52. PMID: 8321321
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Raf-1 Antibody (H-8) | sc-271928 | 200 µg/ml | $322.00 | |||
p-Raf-1 Antibody (H-8): m-IgGκ BP-HRP Bundle | sc-535065 | 200 µg Ab; 40 µg BP | $361.00 | |||
p-Raf-1 Antibody (H-8): m-IgG2b BP-HRP Bundle | sc-549338 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-Raf-1 (H-8) Neutralizing Peptide | sc-271928 P | 100 µg/0.5 ml | $69.00 |