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Raf-1 Antibody (E-10) - Western Blotting - Image 388002
Raf-1 抗体 (E-10) HRP: sc-7267 HRP. F9 (A), 3611-RF (B), KNRK (C) 和 NIH/3T3 (D) 全细胞裂解液中 Raf-1 表达的直接 Western 印迹分析.
Raf-1 抗体 (E-10) HRP: sc-7267 HRP. F9 (A), 3611-RF (B), KNRK (C) 和 NIH/3T3 (D) 全细胞裂解液中 Raf-1 表达的直接 Western 印迹分析.

Raf-1 抗体 (E-10): sc-7267

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说明书
  • Raf-1 抗体 E-10 是小鼠单克隆 IgG1 κ，Raf-1抗体, 在118篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Raf-1的C-terminus的氨基酸637-648.之间
  • Raf-1 抗体 (E-10) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 Raf-1; 也和以下物种反应，包括： 和 equine, canine and bovine
  • 抗Raf-1抗体（E-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Raf-1 (E-10): sc-7267的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Raf-1 抗体 (E-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Raf-1 抗体 (E-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Raf 抗体 包括 A-Raf, Raf-1和Raf-B

Raf-1抗体（E-10）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过WB、IP、IF、IHCP、FCM和ELISA检测小鼠、大鼠和人源的Raf-1蛋白。Raf-1抗体（E-10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。Raf-1，也称为c-Raf或RAF1，是一种重要的丝氨酸/苏氨酸激酶，在细胞分裂、分化及存活所必需的细胞分裂原活化蛋白激酶（MAPK）信号传导通路中发挥着重要作用。Raf-1主要位于细胞质中，作为关键介质将信号从活化的受体酪氨酸激酶传递给下游效应分子，如MEK和ERK。这种定位使Raf-1能够快速响应细胞外信号，确保细胞反应得到适当协调。Raf-1活性的失调与多种癌症有关，因此Raf-1成为治疗干预的关键靶点。Raf-1的失控会导致细胞无序增殖，这凸显了了解信号级联中的调节机制和相互作用的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Raf-1 抗体 (E-10) 参考文献:

  1. 激活 Raf-1 激酶需要 p21ras 和 pp60v-src，但两者都不足以单独激活 Raf-1 激酶。  |  Williams, NG., et al. 1992. Proc Natl Acad Sci U S A. 89: 2922-6. PMID: 1372995
  2. 白细胞介素 2 能诱导 T 细胞系中 p72-74 Raf-1 激酶的酪氨酸磷酸化和活化。  |  Turner, B., et al. 1991. Proc Natl Acad Sci U S A. 88: 1227-31. PMID: 1996324
  3. 减数分裂和有丝分裂细胞周期控制中的蛋白激酶级联。  |  Pelech, SL., et al. 1990. Biochem Cell Biol. 68: 1297-330. PMID: 2085430
  4. c-raf-1 的突变激活和最小转化序列的定义。  |  Heidecker, G., et al. 1990. Mol Cell Biol. 10: 2503-12. PMID: 2188091
  5. 从膜到细胞质的信号转导：生长因子和膜结合的癌基因产物会增加 Raf-1 磷酸化和相关蛋白激酶的活性。  |  Morrison, DK., et al. 1988. Proc Natl Acad Sci U S A. 85: 8855-9. PMID: 3057494
  6. 胰岛素刺激的微管相关蛋白激酶在体内被酪氨酸和苏氨酸磷酸化。  |  Ray, LB. and Sturgill, TW. 1988. Proc Natl Acad Sci U S A. 85: 3753-7. PMID: 3287375
  7. 小鼠 A-raf 的特征，这是一种与 v-raf 癌基因相关的新的癌基因。  |  Huleihel, M., et al. 1986. Mol Cell Biol. 6: 2655-62. PMID: 3491291
  8. TRIM22通过激活MAPK信号传导并加速Raf-1降解来促进胶质母细胞瘤细胞的增殖。  |  Fei, X., et al. 2023. Exp Mol Med. 55: 1203-1217. PMID: 37258577
  9. 阿尔茨海默病中快速加速纤维肉瘤-1（RAF-1）的交叉对话途径  |  Hong, H., et al. 2024. Mol Neurobiol. 61: 2798-2807. PMID: 37940778
  10. PHI-1是蛋白磷酸酶-1的内源性抑制蛋白，也是泛癌症标志物，它能调节Raf-1的蛋白稳态。  |  Kirkbride, JA., et al. 2023. Biomolecules. 13: PMID: 38136612
  11. 由逆转录病毒转导的独特癌基因 v-raf 的结构和生物活性。  |  Rapp, UR., et al. 1983. Proc Natl Acad Sci U S A. 80: 4218-22. PMID: 6308607
  12. Raf-1 调节的复杂性。  |  Morrison, DK. and Cutler, RE. 1997. Curr Opin Cell Biol. 9: 174-9. PMID: 9069260

订购信息

产品名称产品编号规格价格数量收藏夹

Raf-1 抗体 (E-10)

sc-7267
200 µg/ml
$316.00

Raf-1 (E-10): m-IgG Fc BP-HRP 套装

sc-526490
200 µg Ab; 10 µg BP
$354.00

Raf-1 (E-10): m-IgG1 BP-HRP 套装

sc-531863
200 µg Ab; 20 µg BP
$354.00

Raf-1 抗体 (E-10) AC

sc-7267 AC
500 µg/ml, 25% agarose
$416.00

Raf-1 抗体 (E-10) HRP

sc-7267 HRP
200 µg/ml
$316.00

Raf-1 抗体 (E-10) FITC

sc-7267 FITC
200 µg/ml
$330.00

Raf-1 抗体 (E-10) PE

sc-7267 PE
200 µg/ml
$343.00

Raf-1 抗体 (E-10) Alexa Fluor® 488

sc-7267 AF488
200 µg/ml
$357.00

Raf-1 抗体 (E-10) Alexa Fluor® 546

sc-7267 AF546
200 µg/ml
$357.00

Raf-1 抗体 (E-10) Alexa Fluor® 594

sc-7267 AF594
200 µg/ml
$357.00

Raf-1 抗体 (E-10) Alexa Fluor® 647

sc-7267 AF647
200 µg/ml
$357.00

Raf-1 抗体 (E-10) Alexa Fluor® 680

sc-7267 AF680
200 µg/ml
$357.00

Raf-1 抗体 (E-10) Alexa Fluor® 790

sc-7267 AF790
200 µg/ml
$357.00

Raf-1 (E-10) 中和勝肽

sc-7267 P
100 µg/0.5 ml
$68.00

Can Raf-1 Antibody (E-10): sc-7267 be used for detection of Raf-1 of hamster origin?

Asked by: jenni
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support 10
Date published: 2017-01-09

What is the difference between sc-7267 and sc-7267 P?

Asked by: AbPolly
Thank you for your inquiry. sc-7267 is our standard unconjugated Raf-1 (E-10) monoclonal antibody. sc-7267 P is the blocking peptide matching this antibody that can be used as a negative control in combination with the antibody. For the protocol on how to use the blocking peptide, please see here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Service
Date published: 2016-12-23
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Rated 3 out of 5 by from Not very clear blotI detected more bands and a not very clear blot from A549 cells
Date published: 2021-03-17
Rated 5 out of 5 by from Works in WBWe use it in WB and we detected a clear band of the expected molecular weight
Date published: 2020-07-22
Rated 5 out of 5 by from CRAFAplin lab used Santa cruz CRAF, SC-7267 anitbody for western blotting.
Date published: 2019-02-06
Rated 4 out of 5 by from wellWe used this antibody together with p-Raf-1. I didn't detect the band I wanted.
Date published: 2018-02-14
Rated 1 out of 5 by from Didnot work for WBdid not work for western - no signal using human cells
Date published: 2017-11-21
Rated 2 out of 5 by from No detection in mouse thymic epithelial cellsI tested sample and got no band Conditions of WB ; 4-20% SDS-PAGE, Wet transfer (400mA/2hr), 1:400 primary antibody in 5% skim milk.
Date published: 2017-08-31
Rated 4 out of 5 by from GreatAntibody provides clear and specific results,the product is good.
Date published: 2017-07-17
Rated 5 out of 5 by from high effective antibodyVery good results, the antibody works well for western blot.We use 1/500 dilution in 5% milk TBST.
Date published: 2017-02-02
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Raf-1 抗体 (E-10) is rated 4.3 out of 5 by 16.
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