p-nestin Antibody (A-4): sc-377538

p-nestin Antibody (A-4) is a mouse monoclonal IgM kappa antibody that detects Thr 316 phosphorylated Nestin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-p-nestin antibody (A-4) is available as the non-conjugated form. Nestin serves as a crucial intermediate filament protein primarily found in embryonic central nervous system progenitor cells, playing a significant role in structural integrity and function. Nestin′s dynamic presence in the intermediate filament network is particularly important during muscle development, where Nestin interacts with other proteins such as desmin and vimentin to form a robust cytoskeletal framework. Nestin′s ability to co-assemble with vimentin or α-internexin into heterodimer coiled-coil structures is essential for the formation of 10 nm intermediate filaments, which are vital for maintaining cellular shape and resilience. Notably, phosphorylation of Nestin at Thr 316, a site specific to cdc2, is significantly increased during mitosis, marking Nestin as a key indicator of cell cycle progression and a target for cdk5-specific phosphorylation. This post-translational modification not only influences Nestin′s localization within neuroepithelial stem cells and glioma cells but also suggests involvement in critical processes such as axon elongation and growth cone guidance during neuronal differentiation. Nestin expression in the rat adrenal gland, particularly in the zona fasciculata and zona reticularis, highlights diverse roles across different tissues, making p-nestin (A-4) monoclonal antibody an invaluable tool for researchers studying cellular development and pathology.

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p-nestin Antibody (A-4) References:

1. A high molecular weight intermediate filament-associated protein in BHK-21 cells is nestin, a type VI intermediate filament protein. Limited co-assembly in vitro to form heteropolymers with type III vimentin and type IV alpha-internexin.  |  Steinert, PM., et al. 1999. J Biol Chem. 274: 9881-90. PMID: 10092680
2. Mitotic reorganization of the intermediate filament protein nestin involves phosphorylation by cdc2 kinase.  |  Sahlgren, CM., et al. 2001. J Biol Chem. 276: 16456-63. PMID: 11278541
3. Mouse nestin protein localizes in growth cones of P19 neurons and cerebellar granule cells.  |  Yan, Y., et al. 2001. Neurosci Lett. 302: 89-92. PMID: 11290394
4. Nestin as a marker for proliferative endothelium in gliomas.  |  Sugawara, K., et al. 2002. Lab Invest. 82: 345-51. PMID: 11896213
5. Nestin expression in rat adrenal gland.  |  Bertelli, E., et al. 2002. Histochem Cell Biol. 117: 371-7. PMID: 11976910
6. CNS stem cells express a new class of intermediate filament protein.  |  Lendahl, U., et al. 1990. Cell. 60: 585-95. PMID: 1689217
7. Nestin Mediates Hedgehog Pathway Tumorigenesis.  |  Li, P., et al. 2016. Cancer Res. 76: 5573-83. PMID: 27496710
8. Myogenesis and the intermediate filament protein, nestin.  |  Kachinsky, AM., et al. 1994. Dev Biol. 165: 216-28. PMID: 8088440
9. Transient expression of the intermediate filament nestin during skeletal muscle development.  |  Sejersen, T. and Lendahl, U. 1993. J Cell Sci. 106 (Pt 4): 1291-300. PMID: 8126108
10. A rod end deletion in the intermediate filament protein nestin alters its subcellular localization in neuroepithelial cells of transgenic mice.  |  Marvin, MJ., et al. 1998. J Cell Sci. 111 (Pt 14): 1951-61. PMID: 9645943

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-nestin Antibody (A-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-p-nestin Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-nestin Antibody (A-4) Specifications

Alternate Product Name: nestin

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Thr 316

Epitope Target: nestin

Epitope Species: rat

Product Reactivity/Detection: Thr 316 phosphorylated Nestin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for p-nestin Antibody (A-4): P: Blocking Peptide

The preferred nestin antibody is nestin Antibody (10c2): sc-23927.