nestin Antibody (Rat-401): sc-33677
- nestin Antibody (Rat-401) is a mouse monoclonal IgG1 κ, cited in 92 publications, provided at 200 µg/ml
- raised against spinal cord extracts of embryonic rat
- Anti-nestin Antibody (Rat-401) is recommended for detection of nestin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
- Anti-nestin Antibody (Rat-401) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for nestin Antibody (Rat-401) for WB and IHC(P) applications. These reagents are now offered in bundles with nestin Antibody (Rat-401) (see ordering information below).
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SEE ALSO...
Nestin Antibody (Rat-401) is a mouse monoclonal IgG1 kappa light chain antibody that detects nestin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-nestin antibody (Rat-401) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nestin is a crucial intermediate filament protein predominantly expressed in embryonic central nervous system progenitor cells, playing a vital role in structural integrity and function. Nestin expression is essential during muscle development, where nestin interacts with other intermediate filament proteins such as desmin and vimentin, forming a dynamic network that supports cellular architecture. Nestin′s ability to co-assemble with vimentin or α-internexin into heterodimer coiled-coil structures is significant for the formation of 10 nm intermediate filaments, which are critical for maintaining cellular shape and resilience. Alterations in the intermediate filament consensus rod domain of nestin can disrupt localization in central nervous system precursor cells and radial glial cells, highlighting nestin′s importance in cellular organization and signaling. Nestin serves as a marker for neuroepithelial stem cells, glioma cells, and tumor endothelial cells during periods of rapid growth, indicating nestin′s potential role in cancer biology. During neuron differentiation, nestin localizes to growth cones, suggesting influence on axon elongation and guidance, which is crucial for proper neural network formation. In rat adrenal gland, nestin expression is observed in the zona fasciculata and zona reticularis, as well as in dermatomal cells and myoblasts during early stages of myogenesis, underscoring nestin′s diverse roles in various tissues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
nestin Antibody (Rat-401) | sc-33677 | 200 µg/ml | $322.00 | |||
nestin Antibody (Rat-401): m-IgG Fc BP-HRP Bundle | sc-526703 | 200 µg Ab; 10 µg BP | $361.00 | |||
nestin Antibody (Rat-401): m-IgG1 BP-HRP Bundle | sc-532076 | 200 µg Ab; 20 µg BP | $361.00 | |||
nestin Antibody (Rat-401) AC | sc-33677 AC | 500 µg/ml, 25% agarose | $424.00 | |||
nestin Antibody (Rat-401) HRP | sc-33677 HRP | 200 µg/ml | $322.00 | |||
nestin Antibody (Rat-401) FITC | sc-33677 FITC | 200 µg/ml | $336.00 | |||
nestin Antibody (Rat-401) PE | sc-33677 PE | 200 µg/ml | $349.00 | |||
nestin Antibody (Rat-401) Alexa Fluor® 488 | sc-33677 AF488 | 200 µg/ml | $364.00 | |||
nestin Antibody (Rat-401) Alexa Fluor® 546 | sc-33677 AF546 | 200 µg/ml | $364.00 | |||
nestin Antibody (Rat-401) Alexa Fluor® 594 | sc-33677 AF594 | 200 µg/ml | $364.00 | |||
nestin Antibody (Rat-401) Alexa Fluor® 647 | sc-33677 AF647 | 200 µg/ml | $364.00 | |||
nestin Antibody (Rat-401) Alexa Fluor® 680 | sc-33677 AF680 | 200 µg/ml | $364.00 | |||
nestin Antibody (Rat-401) Alexa Fluor® 790 | sc-33677 AF790 | 200 µg/ml | $364.00 |