p-HSP 27抗体 (B-3) 推荐用于 mouse, rat 和 human 来源的Ser 82 phosphorylated HSP 27 WB, IP, IF, IHC(P) 和 ELISA检测

p-HSP 27 抗体 (B-3): sc-166693

p-HSP 27抗体 (B-3) is rated 4.4 out of 5 by 10.
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说明书

    • p-HSP 27抗体(B-3)是小鼠单克隆IgG2b κ, 在7篇文献中引用,规格为200 µg/ml
    • 抗-p-HSP 27 抗体 (B-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Ser 82 phosphorylated HSP 27
    • 抗-p-HSP 27 抗体 (B-3) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取p-HSP 27 (B-3): sc-166693免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

What application is the blocking peptide sc-166693 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from excellent antibody for western blotting Very clear and specific band in western blotting of C2C12, 16-HBE, CFBE cells.
Date published: 2018-08-29
Rated 5 out of 5 by from Muy buen anticuerpo para WB Funciona muy bien en extractos celulares de células AGS
Date published: 2018-01-11
Rated 5 out of 5 by from great antibody for western blot The batch of antibody I have worked well on HeLa and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 1'000 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2018-01-04
Rated 4 out of 5 by from Good antibody There is a very perfect band in my sample, while in some samples, there is another very closed band.
Date published: 2017-12-04
Rated 3 out of 5 by from Pity awful bind for my cells. may be it is a lower expression in my cells. thanks for the antibody.
Date published: 2017-10-05
Rated 5 out of 5 by from quite clear band for wb of rat protein 1:1000 diluted for wb of rat protein ,the band is so clear!
Date published: 2017-07-13
Rated 5 out of 5 by from Very potent and highly specific antibody This monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is highly recommended for Western blotting. There was very strong detection of target ~30 kDa HSP27 in human HeLa cells treated for 2 hours with anisomycin on Western blots, and no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2016-12-04
Rated 4 out of 5 by from Antibody worked well in IF at 1:50 dilution Antibody worked well in IF at 1:50 dilution.
Date published: 2015-07-24
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