Date published: 2026-10-17
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p-HSP 27 Antibody (B-3) - Western Blotting - Image 377112
p-HSP 27 抗体 (B-3): sc-166693. 在 HeLa (A), F9 (B), A-10 (C), MCF7 (D), HUV-EC-C (E) 和 ECV304 (F) 全细胞裂解液中 p-HSP 27 表达的 Western 印迹分析.
p-HSP 27 抗体 (B-3): sc-166693. 在 HeLa (A), F9 (B), A-10 (C), MCF7 (D), HUV-EC-C (E) 和 ECV304 (F) 全细胞裂解液中 p-HSP 27 表达的 Western 印迹分析.

p-HSP 27 抗体 (B-3): sc-166693

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说明书
  • p-HSP 27抗体(B-3)是小鼠单克隆IgG2b κ, 在15篇文献中引用，规格为200 µg/ml
  • 抗-p-HSP 27 抗体 (B-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Ser 82 phosphorylated HSP 27
  • 抗p-HSP 27抗体（B-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 2b BP-HRP">m-IgG2b BP-HRP是p-HSP 27 Antibody (B-3) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与p-HSP 27 Antibody (B-3) 打包提供（请参阅下面的订购信息）。
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関連項目

附加HSP抗体，包括HSC 70, HSP 10, HSP 105, HSP 20, HSP 27, HSP 40, HSP 47, HSP 56, HSP 60, HSP 65, HSP 70, HSP 75, HSP 90, HSP 90α, HSP 90β和HSP 40-4

p-HSP 27抗体（B-3）是一种IgG2bκ小鼠单克隆p-HSP 27抗体（也称为HSPB1抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的Ser 82磷酸化HSP 27。p-HSP 27抗体（B-3）可作为非偶联抗p-HSP 27抗体形式以及多种偶联形式的抗p-HSP 27抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。HSP 27是一种组成型表达的细胞质蛋白，在应激诱导的损伤下共定位于细胞核。热休克、细胞因子和激素是刺激HSP 27合成的因素之一。哺乳动物热休克蛋白HSP 27的细胞内浓度在热休克和其他代谢应激后增加数倍，并且与获得热耐受性密切相关。MAP激酶激活的蛋白激酶-2在丝氨酸残基Ser 15、Ser 78和Ser 82上磷酸化HSP 27，这些残基在体内响应生长因子和热休克而被磷酸化。Ser 15、Ser 78和Ser 82出现在序列基序RXXS中，该基序被核糖体蛋白S6激酶II识别。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-HSP 27 抗体 (B-3) 参考文献:

  1. 确定 MAPKAP 激酶 2 是哺乳动物小型热休克蛋白磷酸化的主要酶。  |  Stokoe, D., et al. 1992. FEBS Lett. 313: 307-13. PMID: 1332886
  2. 人类 HSP27 在丝氨酸 78 和 82 处被热休克激酶和丝裂原活化激酶磷酸化，这些激酶能识别与 S6 激酶 II 相同的氨基酸基团。  |  Landry, J., et al. 1992. J Biol Chem. 267: 794-803. PMID: 1730670
  3. 在多柔比星治疗的乳腺癌细胞中，Hsp-27诱导需要POU4F2/Brn-3b TF，而磷酸化会改变药物治疗后的细胞定位。  |  Fujita, R., et al. 2011. Cell Stress Chaperones. 16: 427-39. PMID: 21279488
  4. 大脑中的热休克蛋白：Hsp70、Hsp27和HO-1（Hsp32）的作用及其治疗潜力。  |  Sharp, FR., et al. 2013. Transl Stroke Res. 4: 685-92. PMID: 24323422
  5. 石房蛤毒素通过p38蛋白激酶途径诱导HSP 27低聚体的解离。  |  Berni, C., et al. 2015. Chem Res Toxicol. 28: 752-64. PMID: 25710824
  6. Hsp-27 和 NF-κB 通路与前列腺癌细胞中 AR/AR-V7 的表达有关。  |  Kiliccioglu, I., et al. 2019. Gene. 697: 138-143. PMID: 30807779
  7. 细胞因子和生长因子诱导人类星形胶质细胞中的 HSP27 磷酸化。  |  Satoh, J. and Kim, SU. 1995. J Neuropathol Exp Neurol. 54: 504-12. PMID: 7541446
  8. 肿瘤坏死因子-α诱导人类 hsp27 的磷酸化, 细胞定位和寡聚化发生变化，而 hsp27 是一种应激蛋白，可使细胞对这种细胞因子产生抵抗力。  |  Mehlen, P., et al. 1995. J Cell Biochem. 58: 248-59. PMID: 7673331
  9. 白细胞介素-1激活了一种新型蛋白激酶级联，导致Hsp27磷酸化。  |  Freshney, NW., et al. 1994. Cell. 78: 1039-49. PMID: 7923354
  10. 热休克蛋白 27,000 (Hsp27) 的生物学和临床意义：综述。  |  Ciocca, DR., et al. 1993. J Natl Cancer Inst. 85: 1558-70. PMID: 8411230

订购信息

产品名称产品编号规格价格数量收藏夹

p-HSP 27 抗体 (B-3)

sc-166693
200 µg/ml
$322.00

p-HSP 27 (B-3): m-IgG2b BP-HRP 套装

sc-548679
200 µg Ab; 10 µg BP
$361.00

p-HSP 27 抗体 (B-3) AC

sc-166693 AC
500 µg/ml, 25% agarose
$424.00

p-HSP 27 抗体 (B-3) HRP

sc-166693 HRP
200 µg/ml
$322.00

p-HSP 27 抗体 (B-3) FITC

sc-166693 FITC
200 µg/ml
$336.00

p-HSP 27 抗体 (B-3) PE

sc-166693 PE
200 µg/ml
$349.00

p-HSP 27 抗体 (B-3) Alexa Fluor® 488

sc-166693 AF488
200 µg/ml
$364.00

p-HSP 27 抗体 (B-3) Alexa Fluor® 546

sc-166693 AF546
200 µg/ml
$364.00

p-HSP 27 抗体 (B-3) Alexa Fluor® 594

sc-166693 AF594
200 µg/ml
$364.00

p-HSP 27 抗体 (B-3) Alexa Fluor® 647

sc-166693 AF647
200 µg/ml
$364.00

p-HSP 27 抗体 (B-3) Alexa Fluor® 680

sc-166693 AF680
200 µg/ml
$364.00

p-HSP 27 抗体 (B-3) Alexa Fluor® 790

sc-166693 AF790
200 µg/ml
$364.00

p-HSP 27 (B-3) 中和勝肽

sc-166693 P
100 µg/0.5 ml
$69.00

What application is the blocking peptide sc-166693 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by Sherry from very good antibodymouse gut tissues, 1:1000 dilution, very strong bands were explored. It can be coupled with sc-13132 (HSP27).
Date published: 2024-01-10
Rated 5 out of 5 by MAO123 from excellent antibody for western blottingVery clear and specific band in western blotting of C2C12, 16-HBE, CFBE cells.
Date published: 2018-08-29
Rated 5 out of 5 by Ink17 from Muy buen anticuerpo para WBFunciona muy bien en extractos celulares de células AGS
Date published: 2018-01-11
Rated 5 out of 5 by HappyFlower from great antibody for western blotThe batch of antibody I have worked well on HeLa and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 1'000 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2018-01-04
Rated 4 out of 5 by light from Good antibodyThere is a very perfect band in my sample, while in some samples, there is another very closed band.
Date published: 2017-12-04
Rated 3 out of 5 by Dunpeng from Pityawful bind for my cells. may be it is a lower expression in my cells. thanks for the antibody.
Date published: 2017-10-05
Rated 5 out of 5 by 小新贝贝 from quite clear band for wb of rat protein1:1000 diluted for wb of rat protein ,the band is so clear!
Date published: 2017-07-13
Rated 5 out of 5 by Steven Pelech at Kinexus from Very potent and highly specific antibodyThis monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is highly recommended for Western blotting. There was very strong detection of target ~30 kDa HSP27 in human HeLa cells treated for 2 hours with anisomycin on Western blots, and no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2016-12-04
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p-HSP 27 抗体 (B-3) is rated 4.5 out of 5 by 11.
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