p-CD3-zeta抗体 (C415.9A) 推荐用于 human 来源的phosphorylated CD3-ζ WB, IP, FCM 和 ELISA检测

p-CD3-ζ 抗体 (C415.9A): sc-9975

p-CD3-ζ抗体 (C415.9A) is rated 4.6 out of 5 by 8.
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说明书

    • 抗-p-CD3-zeta 抗体 C415.9A 是小鼠单克隆 IgG1 κ,p-CD3-ζ抗体, 在14篇文献中引用,规格为200 µg/ml
    • raised against amino acids 52-163 mapping within the cytoplasmic domain of CD3-ζ precursor of human origin
    • p-CD3-zeta 抗体 (C415.9A) 推荐用于WB, IP, FCM 和 ELISA,检测human 来源的 phosphorylated CD3-ζ
    • 抗-p-CD3-zeta 抗体 (C415.9A) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
    • 关于如何获取p-CD3-ζ (C415.9A): sc-9975免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for p-CD3-ζ Antibody (C415.9A) for WB applications. These reagents are now offered in bundles with p-CD3-ζ Antibody (C415.9A) (see ordering information below).

Can p-CD3-ζ (C415.9A): sc-9975 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, p-CD3-ζ (C415.9A): sc-9975 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Reliable Antibody for WB Great antibody for detecting phospho-CD3 via WB. Works well in human samples.
Date published: 2017-01-26
Rated 5 out of 5 by from worked worked
Date published: 2015-05-28
Rated 5 out of 5 by from Published data for WB in human Jurkat E Published data for WB in human Jurkat E-6.1 cells. -SCBT Publication Review
Date published: 2015-03-16
Rated 4 out of 5 by from Strong Strong, positive band in WB in human CD3- transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Published data for IP in human Jurkat Published data for IP in human Jurkat-CEACAM1 cells. -SCBT Publication Review
Date published: 2015-02-04
Rated 5 out of 5 by from Published data for IP and WB in Jurkat cells Published data for IP and WB in Jurkat cells and human peripheral T lymphocytes. -SCBT Publication Review
Date published: 2015-02-01
Rated 5 out of 5 by from Published data for WB in human peripheral Published data for WB in human peripheral blood mononuclear cells (PBMC). -SCBT Publication Review
Date published: 2015-01-13
Rated 3 out of 5 by from Nice intracellular FCM data of fixed and Nice intracellular FCM data of fixed and permeabilized human peripheral blood leukocytes. -SCBT QC
Date published: 2013-11-03
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