m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for p-CD3-ζ Antibody (C415.9A) for WB applications. These reagents are now offered in bundles with p-CD3-ζ Antibody (C415.9A) (see ordering information below).
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Labware ships FedEx Ground free of charge to the contiguous US
p-CD3-ζ Antibody (C415.9A) is a high quality monoclonal p-CD3-zeta antibody (also designated CD247 antibody, or CD3 zeta antibody) suitable for the detection of the p-CD3-zeta protein of human origin. p-CD3-ζ Antibody (C415.9A) is available as both the non-conjugated anti-p-CD3-zeta antibody form, as well as multiple conjugated forms of anti-p-CD3-zeta antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events intointracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (Cluster of Differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (ge), a heterodimer of delta and epsilon chains (δε) and a homodimer of two zeta chains (ζζ) or a heterodimer of zeta and eta chains (ζh) (2-4). The zeta and eta chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The gamma, epsilon and delta chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the zeta chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of ZAP-70′s SH2 domains bound to the zeta chain ITAMs has been solved.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can p-CD3-ζ (C415.9A): sc-9975 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?
Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, p-CD3-ζ (C415.9A): sc-9975 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.