Date published: 2025-9-9

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p-Src 抗体 (H-3): sc-166860

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说明书
  • p-Src抗体(H-3)是小鼠单克隆IgG2b κ, 在26篇文献中引用,规格为200 µg/ml
  • p-Src 抗体 (H-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测 Tyr 530 phosphorylated c-Src of human and rat origin, and Tyr 535 phosphorylated c-Src of mouse origin
  • 抗p-Src抗体(H-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-Src (H-3): sc-166860免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 p-Src 抗体 (H-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-Src 抗体 (H-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

p-c-Src/Src抗体(H-3)是一种IgG2b κ小鼠单克隆p-c-Src抗体,可通过WB、IP、IF、IHC(P)和ELISA检测人和大鼠来源的Tyr 530磷酸化c-Src以及小鼠来源的Tyr 535磷酸化c-Src。p-c-Src/Src抗体(H-3)可作为非偶联抗p-c-Src抗体形式提供,也可作为多种偶联形式的抗p-c-Src抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Src基因家族的主要翻译产物是缺乏跨膜和外部氨基酸序列的膜相关酪氨酸蛋白激酶。凭借其共同的结构基序,Src家族由脊椎动物中的九个成员组成,包括c-Src、c-Yes、Fgr、Yrk、Fyn、Lyn、Hck、Lck和Blk。Src家族激酶包含一个氨基末端细胞膜锚,随后是SH3和SH2结构域,它们传导参与控制各种细胞过程的信号,包括增殖、分化、运动和粘附。Src家族成员通常保持在不活跃状态,并且可以在诸如有丝分裂等细胞事件期间被短暂激活。Src家族激酶的不同亚细胞定位可能对于特定细胞过程的调节是重要的,如有丝分裂、细胞骨架组织和膜转运。c-Src(也称为pp60Src、Src p60和原癌基因酪氨酸蛋白激酶Src)在广泛的组织和细胞类型中表达,尽管在神经组织和血小板中检测到最高水平的c-Src。c-Src可能在与神经元分化和维持成熟神经元细胞功能相关的事件中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Src 抗体 (H-3) 参考文献:

  1. 疱疹病毒 saimiri 蛋白 StpB 与细胞 Src 相关联  |  Hör, S., et al. 2001. J Gen Virol. 82: 339-344. PMID: 11161272
  2. 调节细胞src蛋白的致癌活性需要激酶结构域和C端磷酸化酪氨酸(Tyr-527)之间的正确间距。  |  Cobb, BS., et al. 1991. Mol Cell Biol. 11: 5832-8. PMID: 1719372
  3. 核内磷酸化c-Src与ER阳性乳腺癌患者预后改善有关。  |  Campbell, EJ., et al. 2008. Br J Cancer. 99: 1769-74. PMID: 19018258
  4. 磷酸化c-Src是宫颈鳞状细胞癌患者复发的新型预测因子  |  Hou, T., et al. 2013. Int J Clin Exp Pathol. 6: 1121-7. PMID: 23696930
  5. 细胞src基因产物调节细胞间通信。  |  Azarnia, R., et al. 1988. Science. 239: 398-401. PMID: 2447651
  6. 鞘氨醇激酶 2 可阻止 1-磷酸鞘氨醇受体-2 和酪氨酸 416 磷酸化 c-Src 的核转位,并促进雌激素受体阴性的 MDA-MB-231 乳腺癌细胞生长:1-磷酸鞘氨醇受体-4的作用。  |  Ohotski, J., et al. 2014. Cell Signal. 26: 1040-7. PMID: 24486401
  7. 细胞外囊泡通过 PI3K/AKT/mTOR 途径驱动细胞外囊泡相关 c-Src 和细胞 SRC-1 激活潜伏 HIV-1。  |  Barclay, RA., et al. 2020. Viruses. 12: PMID: 32575590
  8. 磷脂酶C-γ1与病毒和细胞src激酶相关联。  |  Nakanishi, O., et al. 1993. J Biol Chem. 268: 10754-9. PMID: 8388373
  9. 酪氨酸磷酸化c-Src与肥厚心肌细胞骨架的关系。  |  Kuppuswamy, D., et al. 1997. J Biol Chem. 272: 4500-8. PMID: 9020175
  10. 疱疹病毒saimiri A亚群转化蛋白的基因变异及其与细胞src的关联  |  Lee, H., et al. 1997. J Virol. 71: 3817-25. PMID: 9094657

订购信息

产品名称产品编号规格价格数量收藏夹

p-Src 抗体 (H-3)

sc-166860
200 µg/ml
$316.00

p-Src (H-3): m-IgGκ BP-HRP 套装

sc-521777
200 µg Ab, 40 µg BP
$354.00

p-Src (H-3): m-IgG2b BP-HRP 套装

sc-548965
200 µg Ab; 10 µg BP
$354.00

p-Src 抗体 (H-3) AC

sc-166860 AC
500 µg/ml, 25% agarose
$416.00

p-Src 抗体 (H-3) HRP

sc-166860 HRP
200 µg/ml
$316.00

p-Src 抗体 (H-3) FITC

sc-166860 FITC
200 µg/ml
$330.00

p-Src 抗体 (H-3) PE

sc-166860 PE
200 µg/ml
$343.00

p-Src 抗体 (H-3) Alexa Fluor® 488

sc-166860 AF488
200 µg/ml
$357.00

p-Src 抗体 (H-3) Alexa Fluor® 546

sc-166860 AF546
200 µg/ml
$357.00

p-Src 抗体 (H-3) Alexa Fluor® 594

sc-166860 AF594
200 µg/ml
$357.00

p-Src 抗体 (H-3) Alexa Fluor® 647

sc-166860 AF647
200 µg/ml
$357.00

p-Src 抗体 (H-3) Alexa Fluor® 680

sc-166860 AF680
200 µg/ml
$357.00

p-Src 抗体 (H-3) Alexa Fluor® 790

sc-166860 AF790
200 µg/ml
$357.00

p-Src (H-3) 中和勝肽

sc-166860 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-166860 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Worked well in WBThis antibody worked well in WB using human macrophages
Date published: 2025-06-06
Rated 5 out of 5 by from Worked for human tissue samples WBWorked well for human colon cancer cell lysates using WB at 1:200. Had to expose for longer period of time though.
Date published: 2018-03-22
Rated 4 out of 5 by from p-c-Src Antibody(H-3)H-3 is the ideal ab to detect p-c-Src of my sample, but it needs high concentration to start with.
Date published: 2018-03-07
Rated 5 out of 5 by from best phospho srcuse in a experiment of WB. it works very well for me
Date published: 2018-02-21
Rated 3 out of 5 by from seems not good for liver sample testI try to test healthy liver samples, but seems only a very specific band with about 38Kd, but no the target band.
Date published: 2017-12-04
Rated 5 out of 5 by from Good antibodyGives me strong and specific bands. I would recommend it.
Date published: 2017-09-23
Rated 5 out of 5 by from works great on western blot and IHCWe have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date published: 2017-09-20
Rated 5 out of 5 by from sharp bandsThis antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with no background. A non-specific band around 40 kDa was observed.
Date published: 2017-09-14
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p-Src 抗体 (H-3) is rated 4.7 out of 5 by 19.
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