Date published: 2025-9-6

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Src 抗体 (B-12): sc-8056

4.5(25)
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说明书
  • Src 抗体 B-12 是小鼠单克隆 IgG2a κ,Src抗体, 在273篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的c-Src的C-terminus的氨基酸513-537之间
  • Src 抗体 (B-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 c-Src, Yes p62, Fyn p59, c-Fgr p55 and c-Src-2
  • 抗Src抗体(B-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Src (B-12): sc-8056免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 Src 抗体 (B-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Src 抗体 (B-12) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

c-Src抗体(B-12)是一种IgG2a κ小鼠单克隆c-Src抗体(也称为Rous肉瘤(SRC)病毒原癌基因、非受体酪氨酸激酶抗体或SRC抗体),可通过WB、IP、IF、IHC(P)和ELISA检测来自小鼠、大鼠和人类的c-Src蛋白。c-Src抗体(B-12)既可以是非偶联的抗c-Src抗体形式,也可以是多种偶联形式的抗c-Src抗体,包括琼脂、HRP、PE、FITC和多种Alexa Fluor®偶联物。Src基因家族的主要翻译产物是膜相关的酪氨酸蛋白激酶,缺乏跨膜和外部氨基酸序列。凭借其共同的结构基序,Src家族由脊椎动物中的九个成员组成,包括c-Src、c-Yes、Fgr、Yrk、Fyn、Lyn、Hck、Lck和Blk。Src家族激酶包含一个氨基末端细胞膜锚定物,其后跟着SH3和SH2结构域,可转导控制各种细胞过程的信号,包括增殖、分化、运动和粘附。Src家族成员通常保持处于非活性状态,并可在细胞事件(如有丝分裂)中短暂激活。Src家族激酶的不同亚细胞位置可能对具体细胞过程(如有丝分裂、细胞骨架组织和膜转运)的调节至关重要。c-Src(也称为pp60Src、Src p60和原癌基因酪氨酸蛋白激酶Src)在各种组织和细胞类型中表达,尽管在神经组织和血小板中检测到最高水平的c-Src。c-Src可能参与与神经分化和维持成熟神经细胞功能有关的事件。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Src 抗体 (B-12) 参考文献:

  1. SRC家族激酶介导内皮素对上皮Na+通道的抑制作用  |  Gilmore, ES., et al. 2001. J Biol Chem. 276: 42610-7. PMID: 11560932
  2. src 相关酪氨酸蛋白激酶是造血细胞中的信号元件。  |  Eiseman, E. and Bolen, JB. 1990. Cancer Cells. 2: 303-10. PMID: 1704247
  3. 酪氨酸蛋白激酶 Src 家族在 T 淋巴细胞中的表达和相互作用。  |  Bolen, JB., et al. 1991. Adv Cancer Res. 57: 103-49. PMID: 1950702
  4. 神经元表达高水平的结构修饰, 活化形式的 pp60c-src。  |  Brugge, JS., et al. Nature. 316: 554-7. PMID: 2412121
  5. 血小板表达高水平的 pp60c-src 特异性酪氨酸激酶活性。  |  Golden, A., et al. 1986. Proc Natl Acad Sci U S A. 83: 852-6. PMID: 2419901
  6. 大鼠胚胎纹状体神经元分化过程中 pp60c-src 的变化  |  Cartwright, CA., et al. 1987. Mol Cell Biol. 7: 1830-40. PMID: 2439897
  7. 小鼠大脑中两种形式的 pp60c-src 的发育表达。  |  Wiestler, OD. and Walter, G. 1988. Mol Cell Biol. 8: 502-4. PMID: 2447488
  8. PDGF 受体会使 c-Src SH3 结构域中的 Tyr 138 发生磷酸化,从而减少肽配体的结合。  |  Broome, MA. and Hunter, T. 1997. Oncogene. 14: 17-34. PMID: 9010229

订购信息

产品名称产品编号规格价格数量收藏夹

Src 抗体 (B-12)

sc-8056
200 µg/ml
$316.00

Src (B-12): m-IgG Fc BP-HRP 套装

sc-528218
200 µg Ab; 10 µg BP
$354.00

Src (B-12): m-IgGκ BP-HRP 套装

sc-520542
200 µg Ab, 40 µg BP
$354.00

Src (B-12): m-IgG2a BP-HRP 套装

sc-547050
200 µg Ab; 10 µg BP
$354.00

Src 抗体 (B-12) AC

sc-8056 AC
500 µg/ml, 25% agarose
$416.00

Src 抗体 (B-12) HRP

sc-8056 HRP
200 µg/ml
$316.00

Src 抗体 (B-12) FITC

sc-8056 FITC
200 µg/ml
$330.00

Src 抗体 (B-12) PE

sc-8056 PE
200 µg/ml
$343.00

Src 抗体 (B-12) Alexa Fluor® 488

sc-8056 AF488
200 µg/ml
$357.00

Src 抗体 (B-12) Alexa Fluor® 546

sc-8056 AF546
200 µg/ml
$357.00

Src 抗体 (B-12) Alexa Fluor® 594

sc-8056 AF594
200 µg/ml
$357.00

Src 抗体 (B-12) Alexa Fluor® 647

sc-8056 AF647
200 µg/ml
$357.00

Src 抗体 (B-12) Alexa Fluor® 680

sc-8056 AF680
200 µg/ml
$357.00

Src 抗体 (B-12) Alexa Fluor® 790

sc-8056 AF790
200 µg/ml
$357.00

Src (B-12) 中和勝肽

sc-8056 P
100 µg/0.5 ml
$68.00

How does this monoclonal antibody compare with the discontinued rabbit polyclonal, sc-18: c-Src (SRC 2)?

Asked by: TinTin
Thank you for your question. sc-8056: c-Src (B-12) is raised against the same peptide used to develop the rabbit polyclonal antibody (sc-18). This antibody is well characterized with published data in a variety of applications, and is featured in nearly 100 publications.
Answered by: Technical Support
Date published: 2016-12-30
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Rated 3 out of 5 by from 二本のバンドの区別が難しい2000~4000倍希釈で使用しました(1000倍の場合汚くなりました)。 毎回しっかり確認できますが、上下二本のバンド太さが場合によって異なるのが気になります。 それぞれのc-Srcの状態の違いを区別することが困難でした。
Date published: 2023-12-26
Rated 4 out of 5 by from GoodThe antibody was used in different cell lines at the concentration of 1:500 or 1:1000 diluted in TBS 0,1% Tween-20 and used overnight. The signal is good and specific.
Date published: 2021-10-04
Rated 4 out of 5 by from Good antibody for WBWe used this antibody for WB on NSCLC , and it show the strong and clear band.
Date published: 2019-09-23
Rated 5 out of 5 by from Works great for WBWorked great for WB at 1:200 dilution. Will probably work at higher dilutions as well. Used for mouse tissue lysates.
Date published: 2018-03-22
Rated 5 out of 5 by from Great for Western blot in HMvECsWorked well in human microvascular endothelial cells for western blotting
Date published: 2018-02-19
Rated 5 out of 5 by from sharp bandsThis antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with no background. A non-specific band was detected around 40 kDa
Date published: 2017-09-14
Rated 5 out of 5 by from good producti bought this antibody a month a ago and did experiment in hela cell line did a good data
Date published: 2017-09-12
Rated 5 out of 5 by from good antibodyThis is a strong and clean antibody for western blot , nice clean staining!
Date published: 2017-08-25
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Src 抗体 (B-12) is rated 4.5 out of 5 by 25.
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