Date published: 2025-9-9

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Nup133 抗体 (E-6): sc-376763

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说明书
  • Nup133 抗体 E-6 是小鼠单克隆 IgG1 κ,Nup133抗体, 在10篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列813-1156位于human物种的Nup133的C-terminus
  • Nup133 抗体 (E-6) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Nup133
  • 抗Nup133抗体(E-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Nup133 (E-6): sc-376763免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Nup133 Antibody (E-6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Nup133 Antibody (E-6) 打包提供(请参阅下面的订购信息)。

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関連項目

Nup133抗体(E-6)是一种IgG1 κ小鼠单克隆Nup133抗体(也称为Nup133抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Nup133蛋白。Nup133抗体(E-6)既可作为非共轭形式的抗Nup133抗体,也可作为多种共轭形式的抗Nup133抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。核孔复合物(NPCs)是核与细胞质之间大分子双向运动的通道,包含100多个不同的亚基。其中许多属于称为核孔蛋白的家族,其特点是存在O-连接的N-乙酰葡糖胺部分和独特的五肽重复(XFXFG)。核孔复合物蛋白Nup133(核孔蛋白Nup133)位于核孔的细胞质和核两侧,在有丝分裂期间定位于着丝粒。它与Nup160、Nup96和Nup107一起构成Nup160核孔亚复合物的一部分。这种复合物对RNA的输出很重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nup133 抗体 (E-6) 参考文献:

  1. 新型脊椎动物核多聚蛋白 Nup133 和 Nup160 在 mRNA 导出中发挥作用。  |  Vasu, S., et al. 2001. J Cell Biol. 155: 339-54. PMID: 11684705
  2. 单个核孔蛋白Nup107的缺失会阻止部分核孔蛋白组装成核孔复合体。  |  Boehmer, T., et al. 2003. Proc Natl Acad Sci U S A. 100: 981-5. PMID: 12552102
  3. 去除单个核孔亚复合物会导致脊椎动物的细胞核没有核孔。  |  Harel, A., et al. 2003. Mol Cell. 11: 853-64. PMID: 12718872
  4. 对 Nup133 结构域的结构和功能分析揭示了核孔复合体的模块化构件。  |  Berke, IC., et al. 2004. J Cell Biol. 167: 591-7. PMID: 15557116
  5. 依赖于Nup133的NPC锚定网络将原相中的中心体系在核包膜上。  |  Bolhy, S., et al. 2011. J Cell Biol. 192: 855-71. PMID: 21383080
  6. Cdk1激活神经干细胞的细胞分裂前期核膜动力蛋白的招募和顶端核迁移。  |  Baffet, AD., et al. 2015. Dev Cell. 33: 703-16. PMID: 26051540
  7. 周围蛋白质骨架调节了由两亲性螺旋进行的膜曲率感应。  |  Doucet, CM., et al. 2015. PLoS One. 10: e0137965. PMID: 26366573
  8. DNA甲基化、自杀行为和衰老之间的相关性研究。  |  Jeremian, R., et al. 2017. Bipolar Disord. 19: 32-40. PMID: 28276657
  9. 胎儿区类固醇和雌激素对少突胶质细胞前体细胞氧化损伤的性别特异性影响。  |  Sunny, DE., et al. 2021. Int J Mol Sci. 22: PMID: 34205405
  10. 通过整合生物信息学分析鉴定食管癌中新型核孔复合体相关蛋白。  |  Mohamadynejad, P., et al. 2024. J Biomol Struct Dyn. 42: 7221-7232. PMID: 37504972

订购信息

产品名称产品编号规格价格数量收藏夹

Nup133 抗体 (E-6)

sc-376763
200 µg/ml
$316.00

Nup133 (E-6): m-IgG Fc BP-HRP 套装

sc-529931
200 µg Ab; 10 µg BP
$354.00

Nup133 (E-6): m-IgGκ BP-HRP 套装

sc-523086
200 µg Ab, 40 µg BP
$354.00

Nup133 (E-6): m-IgG1 BP-HRP 套装

sc-543771
200 µg Ab; 20 µg BP
$354.00

Nup133 抗体 (E-6) AC

sc-376763 AC
500 µg/ml, 25% agarose
$416.00

Nup133 抗体 (E-6) HRP

sc-376763 HRP
200 µg/ml
$316.00

Nup133 抗体 (E-6) FITC

sc-376763 FITC
200 µg/ml
$330.00

Nup133 抗体 (E-6) PE

sc-376763 PE
200 µg/ml
$343.00

Nup133 抗体 (E-6) Alexa Fluor® 488

sc-376763 AF488
200 µg/ml
$357.00

Nup133 抗体 (E-6) Alexa Fluor® 546

sc-376763 AF546
200 µg/ml
$357.00

Nup133 抗体 (E-6) Alexa Fluor® 594

sc-376763 AF594
200 µg/ml
$357.00

Nup133 抗体 (E-6) Alexa Fluor® 647

sc-376763 AF647
200 µg/ml
$357.00

Nup133 抗体 (E-6) Alexa Fluor® 680

sc-376763 AF680
200 µg/ml
$357.00

Nup133 抗体 (E-6) Alexa Fluor® 790

sc-376763 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with Nup133 (E-6): sc-376763 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from good antibodyI tried it for IF. The antibody gave good and specific signals.
Date published: 2018-06-18
Rated 5 out of 5 by from Works great for IFUsed at 1:100 for IF in MCF10AT cell line. 20min 4%PFA IN 1XPBS 15min 0.5% TritonX100 35 min 5%BSA in PBS+Tween20 0.01% 4C o/n anti Nup205 1:100 in 1%BSA+PBSTw0.01% 1h RT Alexafluor 568 secondary antibodies in 1%BSA+PBSTw0.01% + Hoescht 1:500
Date published: 2018-02-19
Rated 5 out of 5 by from Excellent for WBit works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
Rated 5 out of 5 by from great features!Works well for IF in human HT1080 cancer cells.
Date published: 2017-07-10
Rated 5 out of 5 by from Excellent antibodyThe antibody works beautifully and is very specific.
Date published: 2017-05-11
Rated 5 out of 5 by from Great monoclonal antibodyNup133 Antibody (E-6) is great for Western blot and IF. It produced nice strong and specific band with little to no background and robust immunostain
Date published: 2017-02-10
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclearProduced beautiful immunofluorescence nuclear envelope staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-16
Rated 5 out of 5 by from Good Western Blot data of Nup133 expressionGood Western Blot data of Nup133 expression in HeLa and Hep G2 nuclear extracts. -SCBT QC
Date published: 2013-01-30
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Nup133 抗体 (E-6) is rated 5.0 out of 5 by 8.
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