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Nup133 抗体 (E-4): sc-376699

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说明书
  • Nup133抗体(E-4)是小鼠单克隆IgG1 κ, 在4篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列813-1156位于human物种的Nup133的C-terminus
  • 抗-Nup133 抗体 (E-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Nup133
  • 抗Nup133抗体(E-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Nup133 (E-4): sc-376699免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Nup133 Antibody (E-4) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与Nup133 Antibody (E-4) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

Nup133抗体(E-4)是一种IgG1 κ小鼠单克隆Nup133抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Nup133蛋白。Nup133抗体(E-4)既可作为非共轭形式的抗Nup133抗体,也可作为多种共轭形式的抗Nup133抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。核孔复合物(NPCs)是细胞核与细胞质之间大分子双向运动的通道,由100多个不同的亚基组成。其中许多亚基属于核孔蛋白家族,其特点是含有O-连接的N-乙酰葡糖胺部分和独特的五肽重复序列(XFXFG)。核孔复合物蛋白Nup133(也称为核孔蛋白Nup133)位于核孔的细胞质侧和细胞核侧,在有丝分裂期间定位于着丝粒。它与Nup160、Nup96和Nup107一起构成Nup160核孔亚复合物的一部分。这种复合物在RNA的输出中发挥着重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nup133 抗体 (E-4) 参考文献:

  1. 有丝分裂末期,NPC 蛋白依次被招募到核外围。  |  Bodoor, K., et al. 1999. J Cell Sci. 112 (Pt 13): 2253-64. PMID: 10362555
  2. 进化保守的 NPC 亚复合物,在哺乳动物细胞中部分重新分布到着丝点。  |  Belgareh, N., et al. 2001. J Cell Biol. 154: 1147-60. PMID: 11564755
  3. 新型脊椎动物核多聚蛋白 Nup133 和 Nup160 在 mRNA 导出中发挥作用。  |  Vasu, S., et al. 2001. J Cell Biol. 155: 339-54. PMID: 11684705
  4. 保守的Nup107-160复合物对于核孔复合体的组装至关重要。  |  Walther, TC., et al. 2003. Cell. 113: 195-206. PMID: 12705868
  5. 对 Nup133 结构域的结构和功能分析揭示了核孔复合体的模块化构件。  |  Berke, IC., et al. 2004. J Cell Biol. 167: 591-7. PMID: 15557116
  6. 结构核孔蛋白Nup157/170和Nup133在结构上相关,并且源自第二个祖先元素。  |  Whittle, JRR. and Schwartz, TU. 2009. J Biol Chem. 284: 28442-28452. PMID: 19674973
  7. 多细胞动物核孔复合体组装的细胞周期依赖性差异。  |  Doucet, CM., et al. 2010. Cell. 141: 1030-41. PMID: 20550937
  8. 通过将核孔蛋白异位靶向染色质来分析核孔组装的起始。  |  Schwartz, M., et al. 2015. Nucleus. 6: 40-54. PMID: 25602437
  9. 人类核孔复合物成分NUP43的晶体结构。  |  Xu, C., et al. 2015. FEBS Lett. 589: 3247-53. PMID: 26391640
  10. 核孔复合体亚基 NUP107 双重突变导致早幼年发病的类固醇抵抗性肾病综合征  |  Miyake, N., et al. 2015. Am J Hum Genet. 97: 555-66. PMID: 26411495
  11. FSGS疾病基因产物和核孔蛋白NUP205在调节转录调节因子YAP和TAZ的核定位和活性中的作用。  |  Ester, L., et al. 2023. Hum Mol Genet. 32: 3153-3165. PMID: 37565816
  12. 编码人类核孔复合体蛋白 hnup153 的 cDNA 序列分析。  |  McMorrow, I., et al. 1994. Biochim Biophys Acta. 1217: 219-23. PMID: 8110839

订购信息

产品名称产品编号规格价格数量收藏夹

Nup133 抗体 (E-4)

sc-376699
200 µg/ml
$316.00

Nup133 (E-4): m-IgGκ BP-HRP 套装

sc-523051
200 µg Ab, 40 µg BP
$354.00

Nup133 抗体 (E-4) AC

sc-376699 AC
500 µg/ml, 25% agarose
$416.00

Nup133 抗体 (E-4) HRP

sc-376699 HRP
200 µg/ml
$316.00

Nup133 抗体 (E-4) FITC

sc-376699 FITC
200 µg/ml
$330.00

Nup133 抗体 (E-4) PE

sc-376699 PE
200 µg/ml
$343.00

Nup133 抗体 (E-4) Alexa Fluor® 488

sc-376699 AF488
200 µg/ml
$357.00

Nup133 抗体 (E-4) Alexa Fluor® 546

sc-376699 AF546
200 µg/ml
$357.00

Nup133 抗体 (E-4) Alexa Fluor® 594

sc-376699 AF594
200 µg/ml
$357.00

Nup133 抗体 (E-4) Alexa Fluor® 647

sc-376699 AF647
200 µg/ml
$357.00

Nup133 抗体 (E-4) Alexa Fluor® 680

sc-376699 AF680
200 µg/ml
$357.00

Nup133 抗体 (E-4) Alexa Fluor® 790

sc-376699 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with Nup133 (E-4): sc-376699 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from It works very wellThis antibody works very well on paraffin-embedded human tissue (IHC).
Date published: 2023-03-14
Rated 5 out of 5 by from clear, clean robust signals on 3 different samplesI tried this antibody on 3 different samples: human primary epidermal keratinocyte lysates, HaCat lysates, and mouse epidermal tissue lysates, across 3 protein concentrations and 2 Ab dilutions; the results for all 3 were clear - the antibody detected Nup133 in all conditions, including small ug concentrations and was best at a 1:100 dilution of the antibody.
Date published: 2019-04-23
Rated 5 out of 5 by from Good Antibody for WB detecting Nup133This antibody at 1:200 dilution works great to detect Nup133 in HEK293T cells by western blot.
Date published: 2017-11-21
Rated 5 out of 5 by from Excellent Signal StrengthThis Nup133 antibody shows good band strength in Western Blot and great staining of the nuclear envelope in IF.
Date published: 2017-02-02
Rated 5 out of 5 by from Beautiful immunofluorescence nuclear envelopeBeautiful immunofluorescence nuclear envelope staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-26
Rated 5 out of 5 by from Good Western Blot data of Nup133 expressionGood Western Blot data of Nup133 expression in HeLa and Hep G2 nuclear extracts. -SCBT QC
Date published: 2014-06-24
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Nup133 抗体 (E-4) is rated 5.0 out of 5 by 6.
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