Date published: 2025-9-11
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Nitrotyrosine Antibody (39B6) - Immunohistochemistry - Image 9243
硝基酪氨酸抗体 (39B6): sc-32757. 经福尔马林固定, 石蜡包埋的李斯特菌感染大鼠脑切片的免疫过氧化物酶染色, 显示巨噬细胞呈阳性染色 由伯尔尼大学的 Stephan Christen 博士友情提供.
Nitrotyrosine Antibody (39B6) - Immunohistochemistry - Image 9243
硝基酪氨酸抗体 (39B6): sc-32757. 经福尔马林固定, 石蜡包埋的李斯特菌感染大鼠脑切片的免疫过氧化物酶染色, 显示巨噬细胞呈阳性染色 由伯尔尼大学的 Stephan Christen 博士友情提供.
Nitrotyrosine Antibody (39B6) - Western Blotting - Image 9928
硝基酪氨酸抗体 (39B6): sc-32757. 对照 BSA (A) 和硝化 BSA (B) 的 Western 印迹分析.
Nitrotyrosine Antibody (39B6) - Western Blotting - Image 9244
硝基酪氨酸抗体 (39B6): sc-32757. SIN-1 硝化 BSA (A) 和 HOCl 氧化 BSA (B) 的 Western 印迹分析. 伯尔尼大学的 Stephan Christen 博士友情提供.
硝基酪氨酸抗体 (39B6): sc-32757. 经福尔马林固定, 石蜡包埋的李斯特菌感染大鼠脑切片的免疫过氧化物酶染色, 显示巨噬细胞呈阳性染色 由伯尔尼大学的 Stephan Christen 博士友情提供.

Nitrotyrosine 抗体 (39B6): sc-32757

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说明书
  • Nitrotyrosine 抗体 39B6 是小鼠单克隆 IgG2a κ，Nitrotyrosine抗体, 在156篇文献中引用，规格为200 µg/ml
  • 针对3-Nitrotyrosine
  • Nitrotyrosine 抗体 (39B6) 推荐用于 WB, IP, IF 和 IHC(P)，检测 nitrosylated tyrosine containing proteins
  • 抗Nitrotyrosine抗体（39B6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Nitrotyrosine (39B6): sc-32757的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Nitrotyrosine 抗体 (39B6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Nitrotyrosine 抗体 (39B6) 组合成套装提供。 (请参阅下面的订购信息)。

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研究信息

関連項目

硝基酪氨酸抗体（39B6）是一种小鼠单克隆IgG2a kappa轻链抗体，用于检测非物种反应性应用中的硝基酪氨酸，如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）。抗硝基酪氨酸抗体（39B6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。硝基酪氨酸是炎症和一氧化氮（NO）生成的重要生物标志物，当酪氨酸残基在活性氮的存在下被亚硝化时形成。硝基酪氨酸的存在表明存在氧化应激，并且可以是多种病理过程的稳定产物，包括过氧化亚硝酸盐的形成。硝基酪氨酸水平升高与多种炎症性疾病有关，如败血性休克、类风湿性关节炎、乳糜泻、动脉粥样硬化斑块和慢性肾衰竭，因此硝基酪氨酸成为这些疾病研究的重要目标。了解硝基酪氨酸在这些情况下的作用有助于深入了解NO依赖性损伤机制和整体炎症反应，凸显了硝基酪氨酸在临床和研究中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nitrotyrosine 抗体 (39B6) 参考文献:

  1. 脊髓损伤后 iNOS 和硝基酪氨酸的表达。  |  Xu, J., et al. 2001. J Neurotrauma. 18: 523-32. PMID: 11393255
  2. 用一种新的单克隆抗体免疫检测紫霉素处理过的大鼠肝脏中的 3-硝基酪氨酸：与 HPLC 分析的比较。  |  Girault, I., et al. 2001. Free Radic Biol Med. 31: 1375-87. PMID: 11728809
  3. 一氧化氮、硝基酪氨酸和一氧化氮调节剂在哮喘和慢性阻塞性肺疾病中的作用。  |  Kharitonov, SA. and Barnes, PJ. 2003. Curr Allergy Asthma Rep. 3: 121-9. PMID: 12562551
  4. 嗜酸性粒细胞或含嗜酸性粒细胞组织中的亚硝基酪氨酸免疫组化伪影。  |  Ogino, K., et al. 2002. Free Radic Res. 36: 1163-70. PMID: 12592668
  5. 通过调控过氧化亚硝酸盐生成的中药方剂温肾汤预防过氧化亚硝酸盐诱发的肾损伤。  |  Rhyu, DY., et al. 2002. Free Radic Res. 36: 1261-9. PMID: 12607816
  6. 患有严重支气管肺发育不良的新生儿在吸入一氧化氮后的血浆 3-硝基酪氨酸和预后。  |  Lorch, SA., et al. 2003. Free Radic Biol Med. 34: 1146-52. PMID: 12706495
  7. 循环游离硝基酪氨酸与认知能力下降。  |  Coppola, L., et al. 2010. Acta Neurol Scand. 122: 175-81. PMID: 20003087
  8. 通过免疫印迹结合质谱分析含硝基酪氨酸蛋白的方法。  |  Kohutiar, M. and Eckhardt, A. 2021. Methods Mol Biol. 2276: 383-396. PMID: 34060056
  9. 在整个肌萎缩性脊髓侧索硬化症（ALS）类似疾病中，游离亚硝基酪氨酸水平升高，而不是蛋白质结合的亚硝基酪氨酸或羟自由基水平升高，这表明酪氨酸硝化是一种与 ALS 相关的家族性超氧化物歧化酶 1 突变体的异常体内特性。  |  Bruijn, LI., et al. 1997. Proc Natl Acad Sci U S A. 94: 7606-11. PMID: 9207139
  10. 乳糜泻患者小肠中存在诱导型一氧化氮合酶, 硝基酪氨酸, CD68 和 CD14。  |  ter Steege, J., et al. 1997. Lab Invest. 77: 29-36. PMID: 9251676
  11. 通过新型夹心酶联免疫吸附试验检测乳糜泻患者血浆中的硝基酪氨酸。  |  ter Steege, JC., et al. 1998. Free Radic Biol Med. 25: 953-63. PMID: 9840741
  12. 检测硝基酪氨酸的免疫组化方法。  |  Viera, L., et al. 1999. Methods Enzymol. 301: 373-81. PMID: 9919586

订购信息

产品名称产品编号规格价格数量收藏夹

Nitrotyrosine 抗体 (39B6)

sc-32757
200 µg/ml
$316.00

Nitrotyrosine (39B6): m-IgG Fc BP-HRP 套装

sc-525537
200 µg Ab; 10 µg BP
$354.00

Nitrotyrosine (39B6): m-IgG2a BP-HRP 套装

sc-546308
200 µg Ab; 10 µg BP
$354.00

Nitrotyrosine 抗体 (39B6) AC

sc-32757 AC
500 µg/ml, 25% agarose
$416.00

Nitrotyrosine 抗体 (39B6) HRP

sc-32757 HRP
200 µg/ml
$316.00

Nitrotyrosine 抗体 (39B6) FITC

sc-32757 FITC
200 µg/ml
$330.00

Nitrotyrosine 抗体 (39B6) PE

sc-32757 PE
200 µg/ml
$343.00

Nitrotyrosine 抗体 (39B6) Alexa Fluor® 488

sc-32757 AF488
200 µg/ml
$357.00

Nitrotyrosine 抗体 (39B6) Alexa Fluor® 546

sc-32757 AF546
200 µg/ml
$357.00

Nitrotyrosine 抗体 (39B6) Alexa Fluor® 594

sc-32757 AF594
200 µg/ml
$357.00

Nitrotyrosine 抗体 (39B6) Alexa Fluor® 647

sc-32757 AF647
200 µg/ml
$357.00

Nitrotyrosine 抗体 (39B6) Alexa Fluor® 680

sc-32757 AF680
200 µg/ml
$357.00

Nitrotyrosine 抗体 (39B6) Alexa Fluor® 790

sc-32757 AF790
200 µg/ml
$357.00

hi. sc-32757 product can use for hamsters'?

Asked by: Minji
Thank you for your question. This antibody against Nitrotyrosine is not species dependent. It will detect nitrosylated tyrosine containing proteins of any species.
Answered by: Tech Service
Date published: 2019-09-23

Is it necessary to use a mouse on mouse kit when staining mouse tissues? I've tried this antibody on 5um mouse brain tissue sections multiple times and have only ever gotten background (IF). Can any suggestions be made?

Asked by: JLap23
Thank you for your question. It may not always be necessary to use a mouse on mouse kit for staining mouse tissues. It depends on your secondary antibody, and whether or not you are staining mouse tissues that express high levels of endogneous IgG. This Nitrotyrosine antibody has extensive publications for IHC/IF in mouse tissues that can be referenced. We also offer this antibody directly conjugated to different fluorochromes. Using a directly conjugated primary antibody will eliminate the need for using any anti-mouse IgG secondary antibody, and possible background or non-specific staining from the secondary antibody alone.
Answered by: Technical Support
Date published: 2019-08-17

Es posible usar este Anticuerpo en riñones de ratas?? Cual seria el kit revelador apropiado?

Asked by: Liliana
Hola, Este anticuerpo no es específico para una especie así que trabajará para la rata. Desafortunadamente, ya no ofrecemos los kits de tinción ABC. Pero como un reemplazo podemos ofrecer lo siguiente: m-IgGκ BP-HRP sc-516102 UltraCruz® Blocking Reagent sc-516214 o ImmunoCruz® ABC Kit sc-516216 mas m-IgGκ BP-B sc-516142 This antibody is not species-specific so it will work for rat. Unfortunately, we do not offer the ABC staining kits anymore. But as a replacement we can offer the following: m-IgGκ BP-HRP sc-516102 UltraCruz® Blocking Reagent sc-516214 Or ImmunoCruz® ABC Kit sc-516216 plus m-IgGκ BP-B sc-516142
Answered by: Tech Service 3
Date published: 2017-01-12

I need to use this Nitrotyrosine Antibody (39B6): sc-32757 for detection in samples of rat origin, will it work?

Asked by: cjMara
Thank you for your question. Yes, it will work for rat. This antibody is recommended for detection of nitrosylated tyrosine containing proteins, regardless of species.
Answered by: Technical Support
Date published: 2017-05-19
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Rated 5 out of 5 by from 质量不错抗体质量不错 WB做的很好 组化结果也很好 性价比高 值得推荐 值得推荐值得推荐值得推荐值得推荐值得推荐
Date published: 2021-07-03
Rated 5 out of 5 by from 功能齐全，应用广泛可以用于WB和IF。非常方便和实用。而且效果很好，值得推荐给朋友。嗯嗯嗯嗯能嗯嗯嗯嗯嗯enenenenenenn
Date published: 2021-05-17
Rated 5 out of 5 by from WB和免疫组化均不错小鼠肺组织，wb和免疫组织化学DAB染色都很不错 ffff
Date published: 2019-12-24
Rated 1 out of 5 by from My lot was poorMy lot was poor. I saw no signal even with a commercially-acquired positive control protein preparation. Long exposure times could not fix the issue. The same lysate showed a strong signal using an anti-nitrotyrosine antibody from another vendor.
Date published: 2019-05-29
Rated 1 out of 5 by from Non ha funzionatoHo recentemente acquistato questo prodtto ma utilizzato su lisati cellulari di linee cellule trattate, non sono riuscita ad ottenere fino a ore nessun segnale in western blot, pur avendo provato un controllo positivo di stress ossidativo e diverse diluizioni dell'anticorpo.
Date published: 2019-04-24
Rated 5 out of 5 by from Works well in IPI used it in IP, and I have excellent results. I recommend it.
Date published: 2018-05-29
Rated 5 out of 5 by from Works well for WBThe Ab works well for WB of animal tissue protein extract (by T-PER)
Date published: 2017-08-10
Rated 3 out of 5 by from Good antibodyI bought this antibody, in over all is good, but have a background for IHC stainig.
Date published: 2017-04-28
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Nitrotyrosine 抗体 (39B6) is rated 4.4 out of 5 by 18.
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