Date published: 2025-9-6

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nestin 抗体 (2Q178): sc-58813

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说明书
  • nestin抗体(2Q178)是小鼠单克隆IgG1 κ, 在21篇文献中引用,规格为200 µg/ml
  • 全长天然nestin蛋白的rat来源
  • 推荐用于 mouse, rat 和 human 来源的nestin WB, IP, IF, IHC(P) 和 FCM检测
  • 看 nestin (Rat-401): sc-33677,可查看有标记的 nestin 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP 是 nestin 抗体 (2Q178) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与nestin 抗体 (2Q178) 组合成套装提供。 (请参阅下面的订购信息)。

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Nestin抗体(2Q178)是一种小鼠单克隆IgG1抗体,可通过多种应用检测小鼠、大鼠和人类样本中的nestin,包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)。Nestin是一种重要的中间丝蛋白,主要在胚胎中枢神经系统的祖细胞中表达,在发育过程中,Nestin在维持这些细胞的完整结构和功能方面发挥着至关重要的作用。Nestin在中间丝网络中的动态存在对于肌肉发育至关重要,因为Nestin会与其他蛋白质(如Desmin和Vimentin)聚合,形成异二聚体螺旋线圈结构,有助于形成10 nm的中间丝。Nestin的定位尤为重要;例如,中间丝共识棒状结构域中的nestin缺失会破坏中枢神经系统前体细胞和放射状胶质细胞的定位,这可能对神经发育过程产生重大影响。此外,在快速生长期间,nestin可作为神经上皮干细胞、胶质瘤细胞和肿瘤内皮细胞的标记物,这凸显了nestin在癌症生物学中的重要性。在神经元分化过程中,nestin定位于生长锥,表明其在引导轴突伸长方面具有潜在作用。此外,在大鼠肾上腺束状带和网状带以及成肌早期阶段的皮层细胞和肌原细胞中观察到nestin表达,这表明nestin在不同组织中具有多种功能。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

nestin 抗体 (2Q178)

sc-58813
200 µg/ml
$316.00

nestin (2Q178): m-IgG Fc BP-HRP 套装

sc-539100
200 µg Ab; 10 µg BP
$354.00

I want an antibody to detect nestin by IHC(P) in human sections. The datasheet is contradictory: "recommended for detection of nestin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; non cross-reactive with human nestin". Any explanations?

Asked by: Susan2706
Thank you for your question. This antibody, nestin (2Q178): sc-58813, is reactive with human and is recommended for IHC(P). We apologize for the conflicting information on our product data sheet. We will update the information on the website and data sheet right away.
Answered by: Technical Support
Date published: 2017-06-27

I am using nestin (2Q178): sc-58813 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Worked well with mESCs by IFTo detect the expression of Nestin in mESCs in the treatment with RA by IF, I bought this antibody. The cells were incubated with sc-58813 at a 1/300 dilution overnight at +4°C, and found this antibody worked well.
Date published: 2016-12-06
Rated 5 out of 5 by from Works great in Western BlotWorks great in Western Blot, will buy it again
Date published: 2015-03-24
Rated 5 out of 5 by from Produced exceptional immunofluorescence cytoplasmicProduced exceptional immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-02-24
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nestin 抗体 (2Q178) is rated 4.7 out of 5 by 3.
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