NELF-A Antibody (A-10): sc-271960

NELF-A Antibody (A-10) is a mouse monoclonal IgG1 antibody that detects NELF-A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NELF-A, also known as Negative elongation factor A or WHSC2 (Wolf-Hirschhorn syndrome candidate 2), plays a crucial role in transcription elongation regulation by forming part of the multisubunit NELF complex, which works with DSIF to inhibit RNA polymerase II elongation. This regulation is vital for maintaining proper gene expression, ensuring precisely controlled transcription and preventing gene overexpression or underexpression that could lead to developmental disorders. The human NELF-A gene is located on chromosome 4p16.3 and encodes a 528 amino acid protein predominantly expressed in endothelial cells. Mutations or deletions in this gene are associated with Wolf-Hirschhorn syndrome, a condition characterized by various developmental and mental health challenges, highlighting NELF-A′s importance in normal cellular function and development. Anti-NELF-A antibody (A-10) serves as an essential tool for researchers studying transcription regulation and its implications in health and disease.

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NELF-A Antibody (A-10) References:

1. NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation.  |  Yamaguchi, Y., et al. 1999. Cell. 97: 41-51. PMID: 10199401
2. Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex.  |  Narita, T., et al. 2003. Mol Cell Biol. 23: 1863-73. PMID: 12612062
3. Negative elongation factor NELF represses human immunodeficiency virus transcription by pausing the RNA polymerase II complex.  |  Zhang, Z., et al. 2007. J Biol Chem. 282: 16981-8. PMID: 17442680
4. Nasal embryonic LHRH factor (NELF) mutations in patients with normosmic hypogonadotropic hypogonadism and Kallmann syndrome.  |  Xu, N., et al. 2011. Fertil Steril. 95: 1613-20.e1-7. PMID: 21300340
5. A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.  |  Luo, M., et al. 2013. J Biol Chem. 288: 34055-34072. PMID: 24097989
6. NELF knockout is associated with impaired pubertal development and subfertility.  |  Quaynor, SD., et al. 2015. Mol Cell Endocrinol. 407: 26-36. PMID: 25731822
7. NELF-E is recruited to DNA double-strand break sites to promote transcriptional repression and repair.  |  Awwad, SW., et al. 2017. EMBO Rep. 18: 745-764. PMID: 28336775
8. NELF complex fosters BRCA1 and RAD51 recruitment to DNA damage sites and modulates sensitivity to PARP inhibition.  |  Bishara, LA., et al. 2021. DNA Repair (Amst). 97: 103025. PMID: 33248388
9. Negative elongation factor: a key factor in the maintenance of intestinal epithelial barrier integrity.  |  Gelmez, E., et al. 2022. Cell Mol Immunol. 19: 453-455. PMID: 34992279
10. Regulation of aging by NELF-A and RNA polymerase II elongation.  |  Ong, CT. and Ngian, ZK. 2024. Neural Regen Res. 19: 1415-1416. PMID: 38051874

The preferred NELF-A antibody is NELF-A Antibody (G-11): sc-365004.